Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Potato stem tip detoxification method

A stem tip detoxification and potato technology, applied in the biological field, can solve the problems of incomplete genetic material, small potato stem tip cells, and virus-free seedling variation, achieving high detoxification rate, stable genetic genes, and improved detoxification efficiency Effect

Inactive Publication Date: 2013-07-10
CROP INST SICHUAN PROVINCE ACAD OF AGRI SCI +1
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] 3. Virus-free seedlings may mutate. Some hormones will be added to the medium for callus culture after the shoot tip is peeled off. The meristem may be affected by these hormones. Genetic variation may occur, and the potato shoot tip cells are small, and there may be genetic material incomplete

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Potato stem tip detoxification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] The present invention will be described in detail below in conjunction with specific embodiments.

[0020] The potato stem tip detoxification method of the present invention includes the following steps:

[0021] 1. Plant the original seeds that need to be detoxified in the field.

[0022] 2. Routine management during plant growth.

[0023] 3. Select healthy plants from the potato field during the budding or flowering period, mark them, and harvest half a month earlier than other plants. Each plant is selected to be larger, and the standard and healthy seed potatoes are harvested and stored separately.

[0024] 4. After the seed potatoes germinate, cut the young shoots for virus detection.

[0025] 5. After detecting healthy seed potatoes, re-germination, cut out the big buds, sterilize them with alcohol and mercury, and place them on conventional MS solid medium to make stem seedlings.

[0026] 6. Cut the upper one-third of the tall stem seedlings to preserve the seed, and the rem...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a potato stem tip detoxification method which includes the following steps: A1 sowing pre-basic seed to be detoxified in a field; A2 conducting normal management in the plant growing period; A3 selecting and marking healthy plants from the potato field in the budding period or the flowering period, harvesting the marked plants half a month earlier than other plants and selecting a large-size standard-type healthy potato seed of each plant to be singly harvested and stored; A4 shearing buds to conduct virus detection after the potato seed sprouts; A5 conducting sprouting on the detected healthy potato seeds, shearing large buds which are subjected to alcohol and corrosive sublimate sterilization processing and placing the buds on a common MS solid medium to produce stem seedlings; A6 shearing 1 / 3 upper parts of the growing stem seedlings to conduct breed conservation and conducting virus detection on the surplus 2 / 3 parts; and A7 conducting rapid propagation according to a common method after virus detection is qualified. By means of the potato stem tip detoxification method, potato detoxification test tube seedlings can be obtained in a short period, and the obtained detoxification test tube seedlings are healthy and stable in heredity gene.

Description

Technical field [0001] The invention provides a method for solving the problem of gene mutation and long detoxification cycle caused by stem apex callus in the process of potato stem apex detoxification and detoxification. This technology belongs to the field of biotechnology. Background technique [0002] Potatoes infected with viruses during the planting process directly affected the quality and yield of potatoes, and seriously hindered the production and utilization of potatoes. The application of potato virus-free technology is the main technical measure to solve the degradation of potato seed. Therefore, virus-free seed potatoes are becoming more and more popular among potato growers. my country has also made a lot of research results in potato shoot tip tissue culture, and established virus-free seed potato breeding systems in various places. Potato stem tip tissue culture virus-free seedlings is the foundation of potato virus-free seed tuber industry. High-quality and r...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01G1/00A01C1/00A01H4/00
Inventor 谢开云何卫王克秀胡建军王余明卢学兰唐铭霞贾巍巍马松明刘可心刘一盛
Owner CROP INST SICHUAN PROVINCE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com