Detoxification method of lily bulbs

Abstract

The invention discloses a detoxification method of lily bulbs, wherein the method comprises the following steps: (1) placing the lily bulbs for 40-50 days at the temperature of 3-8 DEG C; (2) selecting the lily bulbs having the bud length of 4-8 cm, stripping outer-layer flakes, leaving bulbus discs with 3-5 inner-layer flakes, placing the bulbus discs into an incubator, and preprocessing, wherein the preprocessing conditions comprise that the illumination intensity is 1500-2000 Lux, the temperature is 35-40 DEG C and the processing time is 2-5 days; (3) selecting a normally growing bulbus disc, stripping a 0.4-0.7 cm stem tip, transferring into a differentiation culture medium, firstly, carrying out dark culture for 1-2 days at the temperature of 23-25 DEG C, then transferring into an illumination incubator with the illumination intensity of 1500-2000 Lux and the illumination time of 12-16 h/d, heating up at a speed of 1-5 DEG C/d, rising the temperature to 35-45 DEG C, and then keeping the temperature and culturing for 35-45 days. Lily bulb viruses are removed by combining heat treatment and chemical treatment, the induction culture period is shortened, and the method also has the advantages of high detoxification rate, high value-added coefficient and the like, and provides a technical basis for culture of lily virus-free plants and promotion of virus-free culture research.

Description

technical field [0001] The invention relates to the field of plant tissue culture rapid propagation technology and biotechnology, in particular to a detoxification method for lily bulbs. Background technique [0002] Lily is the general name of the genus Lilium (Lilium) of the monocot class Liliaceae (Liliaceae), which is a perennial herbaceous flower. There are more than 90 species of Lilium in the world, and about 47 species originate in my country, accounting for 51% of the world total. There are many varieties of lilies, with huge flowers, rich colors, and beautiful flowers. They can be used as cut flowers, potted flowers, and in gardens and green spaces. Most lilies are edible, and they are the best nourishing products. Lily has the meaning of "a hundred years of harmony" and is deeply loved by people. Lilies are mainly distributed in temperate regions such as China, Japan, North America and Europe. In recent years, the production of cut lilies in my country has devel...

AI Technical Summary

Problems solved by technology

At present, there is no report on the patent for the rapid propagation of Lilium japonicus tissue culture, and the literature research is mainly focused on obtaining tissue culture in the form of seedlings. There are few studies on tissue culture products in the form of bulbs. The bulbs of Lilium argentina have many bacteria, and it is very difficult to sterilize the explants. The problem of high pollution rate has not been well resolved.

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