Method of crossbreeding and quickly propagating the lilium formolongi seed by its parents

Abstract

The invention discloses a method for tissue culture rapid propagation and hybrid seed production of a new seed production parent of Lilium chinensis, and belongs to the technical field of plant tissue culture rapid propagation and hybrid seed production. The method comprises: preparation of culture medium, taking immature lily embryos as explants, after induction, multiplication, bulb expansion culture, cold storage, transplanting, and virus detection, and then using non-toxic test tube bulb seedlings for field planting and flowering , Hybrid seed production, to obtain a large number of non-toxic seeds of new iron gun lily. The invention has the advantages of easy inoculation operation, low pollution rate, high survival rate, low seed variation rate, high purity of hybrid seed production, good seed quality, shortened induction culture period, reduced cost, strong practicability, and convenient Promote apps.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture rapid propagation and hybrid seed production, in particular to a method for the parent test tube bulb tissue culture and hybrid seed production method of a lily hybrid combination. Background technique [0002] Lilium formolongi (Lilium formolongi) is an interspecific hybrid between Lilium formolongi and Lilium formosanum (Lilium longflorum×L.formosanum). So far, based on it, a number of fine lines have been selected and bred, mainly for cut flower cultivation. . The biggest difference between this hybrid and ordinary lilies is that it can be propagated by seeds. Its morphological characteristics are similar to iron lily. The flowers have large petals, thick aroma, and white color. Unlike iron lily, the flowers open sideways, and most of them are in full bloom. It also has the characteristics of short growth period and high temperature resistance of Taiwan lily. The flowering period i...

AI Technical Summary

Problems solved by technology

[0004] However, the problems in these two detoxification tissue culture methods are: the former needs to cut very small shoot apex meristems as explants, but the smaller the shoot apex tissue, the lower the survival rate, and the cut shoot apex tissue is too large , and the virus cannot be completely removed, and it is easy to cause pollution. In addition, shoot tip culture generally needs to form callus first, and then differentiate adventitious buds (bulb buds) from the callus. Due to the callus culture stage, it is easy to The occurrence of genetic variation is not conducive to the maintenance of the variety of fine varieties, and the second prolongs the cultivation cycle of tissue culture seedlings; the latter chooses the bulbs that grow robustly and have been tested to be free of viruses, and directly conduct scale induction culture, which is easy to cause endophytic bacteria and fungal contamination, and detoxification is often not complete; therefore, there is still a need for a more effective and simple detoxification method

Therefore, so far, we have not yet seen the use of the immature embryos of the male parent and the female parent of the new iron gun lily hybrid combination to grow into the parent test tube bulb through tissue culture and rapid propagation, and then planted to flowering, pollination, and hybridization to make seeds respectively. Therefore, the development of this technology is of great value in improving the yield and quality of Lily chinensis seed production