30results about How to "High value-added coefficient" patented technology

The invention provides a method for subculturing camphorwood tissue culture seedlings. The formulation of a subculture medium for the culture method comprises 1/2 MS of culture medium, 2.5-4 mg/L of 6-BA (6-Benzyl Aminopurine), 0.1-0.5 mg/L of NAA (Naphthyl Acetic Acid), 6.5 g/L agar and 30 g/L of sucrose. Multiple subcultures are carried out by using the same subculture formulation, the multiplication factor can be increased to achieve the purpose of propagation, industrial production can be carried out, and a great number of nursery stocks are provided for the market. The method for subculturing the camphorwood tissue culture seedlings, provided by the invention, is beneficial to not only simplification of the operating process, but also reduction of the production cost, thereby achieving higher economic benefits.

The invention discloses a tissue culture rapid propagation method of Guangdong anoectochilus roxburghii, which selects an axillary bud or a terminal bud of anoectochilus roxburghii in the Guangdong area in China as an explant and obtains a mass of vegetative propagated progeny plants by inducing the adventitious buds, carrying out proliferation and carrying out rooting cultivation. By adopting the method of the invention, one axillary bud can be induced to generate about 2 to 4 sprouts, after two months, the proliferation coefficient is between 3 and 4, and the rooting rate can reach over 90 percent. Therefore, the method greatly improves the seedling production capacity of Guangdong anoectochilus roxburghii, shortens the seedling culture time, saves the planting cost and provides the technical guarantee for the storage of the genetic resources of Guangdong anoectochilus roxburghii.

The invention discloses a tissue culture rapid propagation method of the traditional Chinese medicine Artemisia annua. The tissue culture rapid propagation method of the traditional Chinese medicine Artemisia annua is to cultivate the terminal buds or axillary buds of the traditional Chinese medicine Artemisia annua in the primary culture medium to obtain sterile seedlings; the primary culture medium is to add 6-BA and GA to the MS medium In the obtained culture medium, the final concentration of the 6-BA is 0.1-2.0 mg/liter, and the final concentration of the GA is 0.2-2.0 mg/liter. The method of the present invention has the following advantages: easy to obtain materials, very easy to obtain aseptic materials, low variation rate, high value-added coefficient, 4-week multiplication multiple can reach 5 to 6 times, the rooting rate of tissue culture seedlings reaches more than 93%, and the transplanting survival rate Up to 95%, with a strong factory production capacity. By using the method of the invention to breed high-yielding Chinese herb Artemisia annua strains, the high artemisinin content of offspring can be stabilized, thereby increasing the artemisinin output.

The invention relates to a detoxification and rapid propagation method of snow white strawberries. The detoxification and rapid propagation method comprises the following steps: S1, explant selectionand surface cleaning, namely selecting 2-4cm stem tips of snow white strawberry grape stems as explants, and carrying out surface cleaning; S2, sterilization, namely placing the surface-cleaned explants on a workbench for sterilization; S3, induced cultivation, namely picking 0.5mm stem tips of the explants by using a dissecting needle under an anatomical lens, and inoculating the 0.5mm stem tipson an induced culture medium for induced culture for 50-60 days to obtain young seedlings of the snow white strawberries; S4, virus detection and seedling selection, namely carrying out virus detection on the young seedlings of the snow white strawberries by using a virus kit, and selecting the young seedlings, reaching the standard, of the snow white strawberries; S5, subculture multiplication culture, namely transplanting the young seedlings, reaching the standard, of the snow white strawberries into a multiplication culture medium, and carrying out subculture once every 30 days for 5-6 times; and S6, rooting culture, namely transplanting young seedlings with the plant heights being 3-5cm of the snow white strawberries into a rooting culture medium for culture for 20-30 days to obtain snow white strawberry plants.

The invention provides a method for breeding wenshan paphiopedilum seedlings by using somatic embryo. After mature wenshan paphiopedilum fruits are sterilized, the fruits are cut apart to obtain seeds, the seeds are inoculated in an induced germination medium to obtain young embryo, and the young embryo is inoculated in a liquid nutrient medium to obtain the somatic embryo; differentiation seedlings are obtained through differentiation cultivation; the differentiation seedlings are inoculated in a root medium to perform root cultivation, and finally the seedlings are exercised and transplanted, namely the wenshan paphiopedilum seedlings are obtained. The method for breeding wenshan paphiopedilum seedlings by using the somatic embryo obtains the young embryo after sterile germination of the wenshan paphiopedilum mature fruits, breaks dormancy of shoots, promotes germination of the seeds and growth of callus, performs rapid cultivation on the plant somatic embryo, enables wenshan paphiopedilum tissue cultivation enhancement factor to be improved to above 5 times from original 1 time, and greatly improves reproduction efficiency of the wenshan paphiopedilum. Survival rate of the wenshan paphiopedilum seedlings after transplanting reaches above 90%.

The invention discloses a detoxification method of lily bulbs, wherein the method comprises the following steps: (1) placing the lily bulbs for 40-50 days at the temperature of 3-8 DEG C; (2) selecting the lily bulbs having the bud length of 4-8 cm, stripping outer-layer flakes, leaving bulbus discs with 3-5 inner-layer flakes, placing the bulbus discs into an incubator, and preprocessing, wherein the preprocessing conditions comprise that the illumination intensity is 1500-2000 Lux, the temperature is 35-40 DEG C and the processing time is 2-5 days; (3) selecting a normally growing bulbus disc, stripping a 0.4-0.7 cm stem tip, transferring into a differentiation culture medium, firstly, carrying out dark culture for 1-2 days at the temperature of 23-25 DEG C, then transferring into an illumination incubator with the illumination intensity of 1500-2000 Lux and the illumination time of 12-16 h/d, heating up at a speed of 1-5 DEG C/d, rising the temperature to 35-45 DEG C, and then keeping the temperature and culturing for 35-45 days. Lily bulb viruses are removed by combining heat treatment and chemical treatment, the induction culture period is shortened, and the method also has the advantages of high detoxification rate, high value-added coefficient and the like, and provides a technical basis for culture of lily virus-free plants and promotion of virus-free culture research.

The invention discloses a stable and efficient anti-browning and tissue culture proliferation method for dendrobenthamia tonkinensis. The anti-browning and tissue culture proliferation method comprises the following steps: 1) taking materials: taking current-year nutritional branches which have no diseases and grow healthy in sunny days from April to May in spring; 2) sterilizing, wherein a sterilization method comprises the step of sterilizing with alcohol for 30s and sterilizing with 0.1 percent mercuric chloride for 7min; 3) establishing a sterile anti-browning system: flatly laying disinfected stem sections on disinfected filtering paper and airing; inoculating the stem sections with a culture medium containing a 200mg.L<-1> PVP (Polyvinyl Pyrrolidone) anti-browning agent; after sprouts grow to 3cm to 4cm, carrying out proliferation culture; (4) carrying out the proliferation culture: inoculating the induced sprouts with a proliferation culture medium (including WPM (Woody Plant Medium), 1.0mg.L-16-BA (Benzylaminopurine) and 0.05mg.L-1NAA (1-Naphthylacetic Acetic Acid)) and carrying out subculture once every other 20 to 25 days. According to the anti-browning and tissue culture proliferation method for the dendrobenthamia tonkinensis, various phases of tissue culture proliferation of the dendrobenthamia tonkinensis are optimized, the browning rate is effectively reduced to 17.87 percent and the proliferation coefficient is improved to reach 3.09.

The invention provides a rapid propagation method for apple tissue culture seedlings. The method comprises the following steps of culturing the apple tissue culture seedlings for 30-40 days by using asubculture multiplication culture medium; then transferring the apple tissue culture seedlings to a rooting culture medium and culturing the apple tissue culture seedlings for 25-35 days; and finallytransplanting the rooted tissue culture seedlings to a transplanting substrate by using a nutrition pot. By utilizing the multiplication culture medium provided by the method, the multiplication coefficient reaches 6.1 on average and is increased by 8.9 % compared with that obtained through the prior art; by utilizing the rooting culture medium provided by the method, the rooting effect is achieved, and the rooting rate of tissue culture seedlings is remarkably improved, which reaches 85 % on average, and is increased by 6.25 % compared with that obtained through the prior art; and by utilizing the transplanting substrate provided by the method, the survival rate of tissue culture seedlings is increased and reaches 75 % or above, the highest survival rate can reach 85 %, and the survivalrate is increased by 6.25 %-7.14 % compared with that obtained through the prior art.