30results about How to "High value-added coefficient" patented technology

The invention provides a method for subculturing camphorwood tissue culture seedlings. The formulation of a subculture medium for the culture method comprises 1 / 2 MS of culture medium, 2.5-4 mg / L of 6-BA (6-Benzyl Aminopurine), 0.1-0.5 mg / L of NAA (Naphthyl Acetic Acid), 6.5 g / L agar and 30 g / L of sucrose. Multiple subcultures are carried out by using the same subculture formulation, the multiplication factor can be increased to achieve the purpose of propagation, industrial production can be carried out, and a great number of nursery stocks are provided for the market. The method for subculturing the camphorwood tissue culture seedlings, provided by the invention, is beneficial to not only simplification of the operating process, but also reduction of the production cost, thereby achieving higher economic benefits.

The invention discloses a tissue culture rapid propagation method of Guangdong anoectochilus roxburghii, which selects an axillary bud or a terminal bud of anoectochilus roxburghii in the Guangdong area in China as an explant and obtains a mass of vegetative propagated progeny plants by inducing the adventitious buds, carrying out proliferation and carrying out rooting cultivation. By adopting the method of the invention, one axillary bud can be induced to generate about 2 to 4 sprouts, after two months, the proliferation coefficient is between 3 and 4, and the rooting rate can reach over 90 percent. Therefore, the method greatly improves the seedling production capacity of Guangdong anoectochilus roxburghii, shortens the seedling culture time, saves the planting cost and provides the technical guarantee for the storage of the genetic resources of Guangdong anoectochilus roxburghii.

The invention discloses a tissue culture rapid propagation method of the traditional Chinese medicine Artemisia annua. The tissue culture rapid propagation method of the traditional Chinese medicine Artemisia annua is to cultivate the terminal buds or axillary buds of the traditional Chinese medicine Artemisia annua in the primary culture medium to obtain sterile seedlings; the primary culture medium is to add 6-BA and GA to the MS medium In the obtained culture medium, the final concentration of the 6-BA is 0.1-2.0 mg / liter, and the final concentration of the GA is 0.2-2.0 mg / liter. The method of the present invention has the following advantages: easy to obtain materials, very easy to obtain aseptic materials, low variation rate, high value-added coefficient, 4-week multiplication multiple can reach 5 to 6 times, the rooting rate of tissue culture seedlings reaches more than 93%, and the transplanting survival rate Up to 95%, with a strong factory production capacity. By using the method of the invention to breed high-yielding Chinese herb Artemisia annua strains, the high artemisinin content of offspring can be stabilized, thereby increasing the artemisinin output.

The invention discloses a tissue culture and rapid propagation culture medium for gerbera jamesonii and a culture method thereof. The tissue culture medium comprises an induction medium, a proliferation medium and a rooting medium. Tender receptacles of gerbera jamesonii are regenerated in vitro by using the culture medium. The technology comprises the processes of induction, proliferation, rooting, acclimatizating and transplanting and the like of an adventitious bud. By adopting the method and formula, not only is the proliferation coefficient of gerbera jamesonii improved and high quality strong seedlings cultured, but also the culture period can be shortened and the survival rate can be improved. Moreover, the gerbera jamesonii further blossoms in advance in about 15 days, the differentiation time of plantlets in plastic tunnel culture can be shortened in advanced and the flower-picking yield and quality can be improved.

The invention discloses a method for performing tissue culture of dendrobium candidum seedlings. The method comprises the following steps: sterilizing: sterilizing an inoculation chamber and inoculators; germinating: selecting dendrobium candidum capsules, disinfecting the capsules, longitudinally cutting up the capsules, taking out seeds by a pair of tweezers, uniformly spreading the seeds in a culture bottle containing germination nutrient solution to perform seed germination culture for 35-40 days, and forming green protocorms; transplanting: moving the protocorms in a culture bottle containing tissue culture nutrient solution to perform protocorm proliferation and differentiation culture for 20-25 days, and obtaining young seedlings; moving the young seedlings to a culture bottle containing the tissue culture nutrient solution to perform seedling proliferation and differentiation culture for 30-35 days, and obtaining plantlets; moving the plantlets to a culture bottle containing the tissue culture nutrient solution to perform strong seedling culture for 60-65 days, and obtaining middle-age seedlings; moving the middle-age seedlings to a culture bottle containing the tissue culture nutrient solution to perform strong seedling culture for 65-70 days, and obtaining the dendrobium candidum seedlings.

The invention relates to the technical field of tissue culture, in particular to a medium and a method for tissue culture of euonymus maackii. The tissue culture medium provided by the invention is applicable to a variety, namely 'Jinzhi Yuye' (euonymus macckii rupr.), of the euonymus maackii; on the basis of a 1 / 2MS medium, KT, NAA, TDZ and gibberellin are added for promoting callus induction; on the basis of an MS medium, KT and TDZ are added for promoting axillary bud induction; and a WPM medium is taken as a seedling hardening medium. Induced by the medium provided by the invention, a growth coefficient can be increased to 9.72, and a survival rate of seedlings can reach 93% or above.

The invention relates to a detoxification and rapid propagation method of snow white strawberries. The detoxification and rapid propagation method comprises the following steps: S1, explant selectionand surface cleaning, namely selecting 2-4cm stem tips of snow white strawberry grape stems as explants, and carrying out surface cleaning; S2, sterilization, namely placing the surface-cleaned explants on a workbench for sterilization; S3, induced cultivation, namely picking 0.5mm stem tips of the explants by using a dissecting needle under an anatomical lens, and inoculating the 0.5mm stem tipson an induced culture medium for induced culture for 50-60 days to obtain young seedlings of the snow white strawberries; S4, virus detection and seedling selection, namely carrying out virus detection on the young seedlings of the snow white strawberries by using a virus kit, and selecting the young seedlings, reaching the standard, of the snow white strawberries; S5, subculture multiplication culture, namely transplanting the young seedlings, reaching the standard, of the snow white strawberries into a multiplication culture medium, and carrying out subculture once every 30 days for 5-6 times; and S6, rooting culture, namely transplanting young seedlings with the plant heights being 3-5cm of the snow white strawberries into a rooting culture medium for culture for 20-30 days to obtain snow white strawberry plants.

The invention provides a tissue culture method of ilex verticillata. The tissue culture method comprises the following steps: (1) selecting an explant from a female plant; (2) processing the explant, namely sterilizing the explant; (3) performing inducing culturing on buds; (4) culturing the buds in enrichment; and (5) performing inducing culturing on roots. The tissue culture method disclosed by the invention is high in survival rate and high in reproduction speed, the growth coefficient achieves 6.1, the rooting percentage achieves 95.2%, and the ilex verticillata female young plant with pure quality is obtained. The method is free from season limitations, and can be used for rapidly propagating the ilex verticillata throughout the year, so that the large production of the ilex verticillata is realized, and an available method is provided for the rapid extension of the ilex verticillata.

The invention provides a method for breeding wenshan paphiopedilum seedlings by using somatic embryo. After mature wenshan paphiopedilum fruits are sterilized, the fruits are cut apart to obtain seeds, the seeds are inoculated in an induced germination medium to obtain young embryo, and the young embryo is inoculated in a liquid nutrient medium to obtain the somatic embryo; differentiation seedlings are obtained through differentiation cultivation; the differentiation seedlings are inoculated in a root medium to perform root cultivation, and finally the seedlings are exercised and transplanted, namely the wenshan paphiopedilum seedlings are obtained. The method for breeding wenshan paphiopedilum seedlings by using the somatic embryo obtains the young embryo after sterile germination of the wenshan paphiopedilum mature fruits, breaks dormancy of shoots, promotes germination of the seeds and growth of callus, performs rapid cultivation on the plant somatic embryo, enables wenshan paphiopedilum tissue cultivation enhancement factor to be improved to above 5 times from original 1 time, and greatly improves reproduction efficiency of the wenshan paphiopedilum. Survival rate of the wenshan paphiopedilum seedlings after transplanting reaches above 90%.

The invention discloses a detoxification method of lily bulbs, wherein the method comprises the following steps: (1) placing the lily bulbs for 40-50 days at the temperature of 3-8 DEG C; (2) selecting the lily bulbs having the bud length of 4-8 cm, stripping outer-layer flakes, leaving bulbus discs with 3-5 inner-layer flakes, placing the bulbus discs into an incubator, and preprocessing, wherein the preprocessing conditions comprise that the illumination intensity is 1500-2000 Lux, the temperature is 35-40 DEG C and the processing time is 2-5 days; (3) selecting a normally growing bulbus disc, stripping a 0.4-0.7 cm stem tip, transferring into a differentiation culture medium, firstly, carrying out dark culture for 1-2 days at the temperature of 23-25 DEG C, then transferring into an illumination incubator with the illumination intensity of 1500-2000 Lux and the illumination time of 12-16 h / d, heating up at a speed of 1-5 DEG C / d, rising the temperature to 35-45 DEG C, and then keeping the temperature and culturing for 35-45 days. Lily bulb viruses are removed by combining heat treatment and chemical treatment, the induction culture period is shortened, and the method also has the advantages of high detoxification rate, high value-added coefficient and the like, and provides a technical basis for culture of lily virus-free plants and promotion of virus-free culture research.

The invention discloses a stable and efficient anti-browning and tissue culture proliferation method for dendrobenthamia tonkinensis. The anti-browning and tissue culture proliferation method comprises the following steps: 1) taking materials: taking current-year nutritional branches which have no diseases and grow healthy in sunny days from April to May in spring; 2) sterilizing, wherein a sterilization method comprises the step of sterilizing with alcohol for 30s and sterilizing with 0.1 percent mercuric chloride for 7min; 3) establishing a sterile anti-browning system: flatly laying disinfected stem sections on disinfected filtering paper and airing; inoculating the stem sections with a culture medium containing a 200mg.L<-1> PVP (Polyvinyl Pyrrolidone) anti-browning agent; after sprouts grow to 3cm to 4cm, carrying out proliferation culture; (4) carrying out the proliferation culture: inoculating the induced sprouts with a proliferation culture medium (including WPM (Woody Plant Medium), 1.0mg.L-16-BA (Benzylaminopurine) and 0.05mg.L-1NAA (1-Naphthylacetic Acetic Acid)) and carrying out subculture once every other 20 to 25 days. According to the anti-browning and tissue culture proliferation method for the dendrobenthamia tonkinensis, various phases of tissue culture proliferation of the dendrobenthamia tonkinensis are optimized, the browning rate is effectively reduced to 17.87 percent and the proliferation coefficient is improved to reach 3.09.

The invention discloses a method for in vitro cultivation and regeneration of curculigo grass leaves, comprising the steps of selecting explants, cleaning and disinfecting the explants, inducing clustered buds, proliferating clustered buds, and rooting. The present invention successfully establishes a low-cost tissue culture and rapid propagation system of curculigo, which lays a technical foundation for realizing the protection, artificial cultivation, seedling cultivation and establishment of gene banks of curculigo wild resources, and solves the problem of over-exploitation of curculigo wild resources, The supply of medicinal materials is insufficient, the planting is not in scale, the planting is limited by the supply of seedlings, and the quality of medicinal materials is unstable.

The invention relates to a core-drilling seedling raising method for pineapples, which comprises the following steps: 1, raw material selection, core drilling of stock plants, cleaning and disinfection, hormone germination accelerating treatment, stock plant culture, bud suction selection, stock plant treatment and seedling field planting. According to the core-drilling seedling raising method for then pineapples, when the pineapples grow to a certain number of leaves, core drilling treatment is carried out, growing points are destroyed, apical dominance of plants is destroyed, axillary buds of the pineapple are promoted to grow to form seedlings, the cultivated seedlings are uniform in size, robust and consistent in growth, the seedlings are harvested twice, the propagation multiple is 6-8 times, the seedling raising period is 15-16 months, by the adoption of the method, the problem that the number of introduced good variety seedlings is small can be solved, the seedlings are rapidly supplied, and the problem that the propagation multiple of conventional fruit harvesting and seedling raising is low is solved. By means of the seedling raising method, seedlings can emerge quickly and efficiently, and grown fine buds are thick and strong in rhizome, high in multiplication coefficient and high in survival rate and can reach the level of imported seedlings.

The invention discloses a detoxification method of lily bulbs, wherein the method comprises the following steps: (1) placing the lily bulbs for 40-50 days at the temperature of 3-8 DEG C; (2) selecting the lily bulbs having the bud length of 4-8 cm, stripping outer-layer flakes, leaving bulbus discs with 3-5 inner-layer flakes, placing the bulbus discs into an incubator, and preprocessing, wherein the preprocessing conditions comprise that the illumination intensity is 1500-2000 Lux, the temperature is 35-40 DEG C and the processing time is 2-5 days; (3) selecting a normally growing bulbus disc, stripping a 0.4-0.7 cm stem tip, transferring into a differentiation culture medium, firstly, carrying out dark culture for 1-2 days at the temperature of 23-25 DEG C, then transferring into an illumination incubator with the illumination intensity of 1500-2000 Lux and the illumination time of 12-16 h / d, heating up at a speed of 1-5 DEG C / d, rising the temperature to 35-45 DEG C, and then keeping the temperature and culturing for 35-45 days. Lily bulb viruses are removed by combining heat treatment and chemical treatment, the induction culture period is shortened, and the method also has the advantages of high detoxification rate, high value-added coefficient and the like, and provides a technical basis for culture of lily virus-free plants and promotion of virus-free culture research.

The invention relates to the technical field of tissue culture of helleborus thibetanus, in particular to a breeding method of helleborus thibetanus seedlings, which specifically comprises the following steps: S1, selection and treatment of explants; s2, induction of sterile test-tube plantlets; s3, proliferation culture of test-tube plantlets; the method is easy to operate, the culture medium is convenient to prepare, explant materials are easy to obtain, the inductivity, the multiplication coefficient and the rooting rate are high, regenerated plants reserve the excellent characters of female parents, plant regeneration of the tissue culture seedlings of the helleborus thibetanus is achieved, and the method is an effective way for breeding excellent varieties of the helleborus thibetanus.

The invention discloses a tissue culture rapid propagation method of Guangdong anoectochilus roxburghii, which selects an axillary bud or a terminal bud of anoectochilus roxburghii in the Guangdong area in China as an explant and obtains a mass of vegetative propagated progeny plants by inducing the adventitious buds, carrying out proliferation and carrying out rooting cultivation. By adopting the method of the invention, one axillary bud can be induced to generate about 2 to 4 sprouts, after two months, the proliferation coefficient is between 3 and 4, and the rooting rate can reach over 90 percent. Therefore, the method greatly improves the seedling production capacity of Guangdong anoectochilus roxburghii, shortens the seedling culture time, saves the planting cost and provides the technical guarantee for the storage of the genetic resources of Guangdong anoectochilus roxburghii.

The invention belongs to the technical field of plant tissue culture, and provides a tissue culture method using Pan's Bingdeng Yulu inflorescence rachis as explants, comprising the following steps: selecting healthy Yulu inflorescence rachis, cleaning and disinfecting, and obtaining aseptic explants Inoculating the aseptic explant into a callus-inducing medium to induce callus after cutting into sections; then cutting the callus into small pieces and transferring it to an adventitious bud differentiation medium for differentiation and cultivation to obtain clustered buds; Then, after cultivating the strong seedlings of the ramets of clustered buds, they are transferred to the rooting medium for cultivation to obtain complete plants with roots, and finally the seedlings are hardened and cultivated. The present invention obtains Yulu tissue culture seedlings by in vitro culture of Yulu inflorescence rachis tissue, the method is easy to obtain materials, is convenient for disinfection, has high callus differentiation rate, high rooting rate, ensures strain advantage, and can rapidly and massively reproduce a large number of excellent plants. Seedlings of Bingdeng Yulu.

The invention provides a method for improving regeneration efficiency in the tissue culture process of Cinnamomum camphora. First, sterilize the seeds of Cinnamomum camphora, and germinate the sterilized seeds to obtain aseptic Cinnamomum camphora seedlings; then, use the aseptic Cinnamomum camphora seedlings As explants, the corresponding Cinnamomum camphora tissue culture method is established through the steps of "induction of buds", "proliferation of buds" and "rooting of buds"; wherein, in the step of "proliferation of buds" Activated carbon was added as an additional component in the "propagation medium" used and the "rooting medium" used in the "rooting of shoots" step. The present invention has the advantages that: (1) active carbon is added in part of the culture medium to absorb metabolites, so that the value-added coefficient of tissue culture seedlings is increased, the browning rate is reduced, and the rooting rate is increased, thereby improving the regeneration efficiency of camphor tissue culture; (2) Choose to sterilize the seeds to effectively avoid the damage caused by the "disinfection process" to the young and tender stems of the materials required in subsequent experiments.

The present invention relates to a formulation of subculture medium for tissue cultured seedlings of Pearl Color Osmanthus and its application. The components of the medium include woody plant medium WPM, cytokinin 6-BA, kinetin KT and gibberellin GA 3 , including a final concentration of 6.5g·L ‑1 agar and a final concentration of 30g·L ‑1 sucrose; after analysis, the best medium for the proliferation of Zhenzhu Caigui is: woody plant medium WPM, final concentration 4.0 mg·L ‑1 Cytokinin 6-BA, final concentration 2.0 mg·L ‑1 Kinetin KT, final concentration 0.5 mg·L ‑1 gibberellin GA3, final concentration 6.5g L ‑1 Agar, final concentration 30g L ‑1 sucrose; under this condition, the multiplication coefficient of Pearl Caigui tissue culture seedlings can reach 5.8.

The invention discloses an induction method for a grape fruit callus and a culture medium thereof. The culture medium comprises the composition: a basal culture medium, 0.8-1.2 mg / L of TDZ, 0.3-0.5 mg / L of IBA, 18-22 g / L of sugar, and 5-7 g / L of agar. The induction method is characterized by comprising the steps: sterilizing grape fruits, then peeling, taking pulp blocks, inoculating the inductionculture medium with the pulp blocks, and carrying out induction culture until the callus is formed. The culture method of the fruit callus of 'Xiahei' grapes is successfully established, and the induction rate of the fruit callus reaches 100%, a new way is provided for molecular biology and genetic engineering research of the 'Xiahei' grapes, a new experimental material is provided for the genetic engineering research and gene transformation of the grapes, and meanwhile, a reference is also provided for culture of the grape fruit callus.

The invention discloses a method for improving tissue culture, proliferation and seedling expansion effects of a bamboo-root ginger. The method comprises the following steps: cluster bud induction, cluster bud subculture multiplication, tube seedling cultivation, tube seedling propagation and the like, wherein in the cluster bud induction, the subculture medium is obtained by the following steps: by taking MS as a basic culture medium, inoculating 1.5mg / L of BA into the basic culture medium, and transferring to the basic culture medium containing 1.5mg / L of BA and 0.2mg / L of NAA after 20d, wherein the concentration of NH4NO3 in the MS culture medium of the proliferation medium is 1 / 2 to 1 / 3; the cutting method of tube seedling propagation comprises the following steps: dividing a test tube cluster seedling into 2-3 adventitious buds; removing the part which is 0.5cm over the basal part of a stem; and forming micro block mass and transferring to the proliferation medium for breeding. The defects that existing bamboo-root ginger easily generates low proliferation coefficient, vitrification and yellowing in a long-term in-vitro culture process are overcome; the method for improving the tissue culture, proliferation and seedling expansion effects of the bamboo-root ginger is high in proliferation coefficient; and vitrification and yellowing are effectively reduced.

The invention discloses a peach fruit callus tissue culture medium and a culture method thereof. The culture medium comprises the following components: an improved WPM culture medium as a basic culture medium, a plant-growth regulator, 20-40 g / L of cane sugar, and 4.5-6.5 g / L of agar powder, wherein the pH value is 5.5-6.0. The culture method comprises the following steps: 1) bacteria-free treatment on peach fruits, and 2) round slice culture on the peach fruits. The callus induction rate of the peach fruits is greatly increased by the callus tissue culture medium and the culture method; a lot of high-quality callus tissues can be obtained in a short period by culturing the callus tissues of the peach pulp, problems of inconsistent callus tissue states in culture of peach tree leaves and low success rate of callus tissue culture can be solved, the method provides an effective approach for large-scale culture of the callus tissue, and provides a novel approach for molecular biology and gene engineering research of peaches.

The present invention provides a kind of rapid propagation method of schizomaea, comprising step 1: explant selection and sterilization; step 2, sterilized seeds are inoculated on the callus induction medium, and each bottle is inoculated with 10 Block explants, adjust the pH of the medium to 5.8, place them in dark culture at 25°C, and start to form callus after 5 to 7 days of culture; step 3, under dark culture conditions, the cultured on the callus medium Embryogenic callus, after peeling off, transferred to the subculture medium for culture, the pH of the medium was adjusted to 5.8, the temperature was 25±1°C, and every 20d to 28d was a multiplication cycle; step 4: bud differentiation, step 5: rooting and Proliferation; Step six: transplanting. The method for propagating the grass grass provided by the invention has the advantages of simple sampling, high induction rate of the cultured callus, high differentiation rate, high value-added coefficient, good stability, and can obtain a large amount of robust test-tube plantlets in a relatively short period of time, and The method has strong operability, low production cost and high application value, and is very suitable for industrial production.

The invention relates to the technical field of tissue culture, in particular to a medium and a method for tissue culture of euonymus maackii. The tissue culture medium provided by the invention is applicable to a variety, namely 'Jinzhi Yuye' (euonymus macckii rupr.), of the euonymus maackii; on the basis of a 1 / 2MS medium, KT, NAA, TDZ and gibberellin are added for promoting callus induction; on the basis of an MS medium, KT and TDZ are added for promoting axillary bud induction; and a WPM medium is taken as a seedling hardening medium. Induced by the medium provided by the invention, a growth coefficient can be increased to 9.72, and a survival rate of seedlings can reach 93% or above.

The invention provides a rapid propagation method for apple tissue culture seedlings. The method comprises the following steps of culturing the apple tissue culture seedlings for 30-40 days by using asubculture multiplication culture medium; then transferring the apple tissue culture seedlings to a rooting culture medium and culturing the apple tissue culture seedlings for 25-35 days; and finallytransplanting the rooted tissue culture seedlings to a transplanting substrate by using a nutrition pot. By utilizing the multiplication culture medium provided by the method, the multiplication coefficient reaches 6.1 on average and is increased by 8.9 % compared with that obtained through the prior art; by utilizing the rooting culture medium provided by the method, the rooting effect is achieved, and the rooting rate of tissue culture seedlings is remarkably improved, which reaches 85 % on average, and is increased by 6.25 % compared with that obtained through the prior art; and by utilizing the transplanting substrate provided by the method, the survival rate of tissue culture seedlings is increased and reaches 75 % or above, the highest survival rate can reach 85 %, and the survivalrate is increased by 6.25 %-7.14 % compared with that obtained through the prior art.

A lilium duchartrei franch tissue culture rapid propagation and separation preservation method comprises the steps of obtaining explant, inducing adventitious buds, carrying out enrichment culture, enlarging the bulb, carrying out rooting culture, carrying out separation preservation and carrying out transplanting. A scale of lilium duchartrei franch serves as the explant, a tissue culture rapid propagation and separation preservation system of the lilium duchartrei franch is established, the appreciation coefficient highly reaches 1:10, and the separation preservation subculture period reaches four years or longer. By means of the method, the problem of tissue culture rapid propagation of the lilium duchartrei franch is effectively solved, the separation preservation period is greatly prolonged, the preservation, the development and the utilization of the germplasm resources of the lilium duchartrei franch are facilitated, and a foundation is laid for breeding of the lilium duchartrei franch.

The invention discloses a method for inducing embryoid bodies of cabbage, which includes induction of sterile seedlings, induction of embryoid bodies, induction of regenerated plants, seedling hardening and transplantation. directly induce regeneration plants or induce embryoid bodies or induce budding, and the induction rate is not high; the following hypocotyls of the present invention are used as explants for inducing embryoid bodies, and the induction rate can reach 90%, and the embryoid bodies induce regenerated plants. The induction rate can reach 90%; the regenerated plants are induced by embryoid bodies, and the regenerated plants have high genetic stability, no physiological isolation from the mother body, and a high value-added coefficient.

The invention provides a method for breeding wenshan paphiopedilum seedlings by using somatic embryo. After mature wenshan paphiopedilum fruits are sterilized, the fruits are cut apart to obtain seeds, the seeds are inoculated in an induced germination medium to obtain young embryo, and the young embryo is inoculated in a liquid nutrient medium to obtain the somatic embryo; differentiation seedlings are obtained through differentiation cultivation; the differentiation seedlings are inoculated in a root medium to perform root cultivation, and finally the seedlings are exercised and transplanted, namely the wenshan paphiopedilum seedlings are obtained. The method for breeding wenshan paphiopedilum seedlings by using the somatic embryo obtains the young embryo after sterile germination of the wenshan paphiopedilum mature fruits, breaks dormancy of shoots, promotes germination of the seeds and growth of callus, performs rapid cultivation on the plant somatic embryo, enables wenshan paphiopedilum tissue cultivation enhancement factor to be improved to above 5 times from original 1 time, and greatly improves reproduction efficiency of the wenshan paphiopedilum. Survival rate of the wenshan paphiopedilum seedlings after transplanting reaches above 90%.