30results about How to "High value-added coefficient" patented technology

The invention provides a tissue culture method of ilex verticillata. The tissue culture method comprises the following steps: (1) selecting an explant from a female plant; (2) processing the explant, namely sterilizing the explant; (3) performing inducing culturing on buds; (4) culturing the buds in enrichment; and (5) performing inducing culturing on roots. The tissue culture method disclosed by the invention is high in survival rate and high in reproduction speed, the growth coefficient achieves 6.1, the rooting percentage achieves 95.2%, and the ilex verticillata female young plant with pure quality is obtained. The method is free from season limitations, and can be used for rapidly propagating the ilex verticillata throughout the year, so that the large production of the ilex verticillata is realized, and an available method is provided for the rapid extension of the ilex verticillata.

The invention discloses a method for in vitro cultivation and regeneration of curculigo grass leaves, comprising the steps of selecting explants, cleaning and disinfecting the explants, inducing clustered buds, proliferating clustered buds, and rooting. The present invention successfully establishes a low-cost tissue culture and rapid propagation system of curculigo, which lays a technical foundation for realizing the protection, artificial cultivation, seedling cultivation and establishment of gene banks of curculigo wild resources, and solves the problem of over-exploitation of curculigo wild resources, The supply of medicinal materials is insufficient, the planting is not in scale, the planting is limited by the supply of seedlings, and the quality of medicinal materials is unstable.

The invention discloses a detoxification method of lily bulbs, wherein the method comprises the following steps: (1) placing the lily bulbs for 40-50 days at the temperature of 3-8 DEG C; (2) selecting the lily bulbs having the bud length of 4-8 cm, stripping outer-layer flakes, leaving bulbus discs with 3-5 inner-layer flakes, placing the bulbus discs into an incubator, and preprocessing, wherein the preprocessing conditions comprise that the illumination intensity is 1500-2000 Lux, the temperature is 35-40 DEG C and the processing time is 2-5 days; (3) selecting a normally growing bulbus disc, stripping a 0.4-0.7 cm stem tip, transferring into a differentiation culture medium, firstly, carrying out dark culture for 1-2 days at the temperature of 23-25 DEG C, then transferring into an illumination incubator with the illumination intensity of 1500-2000 Lux and the illumination time of 12-16 h / d, heating up at a speed of 1-5 DEG C / d, rising the temperature to 35-45 DEG C, and then keeping the temperature and culturing for 35-45 days. Lily bulb viruses are removed by combining heat treatment and chemical treatment, the induction culture period is shortened, and the method also has the advantages of high detoxification rate, high value-added coefficient and the like, and provides a technical basis for culture of lily virus-free plants and promotion of virus-free culture research.

The invention discloses a fast propagation method of tissue culture of traditional Chinese medicine Artemisia annua. The fast propagation method of tissue culture of traditional Chinese medicine Artemisia annua is that culture a terminal bud or an axillary bud of Artemisia annua are cultured in an initial medium so as to obtain an aseptic seedling. The initial medium is a medium of MS medium added with 6-BA and GA. The final concentration of the 6-BA is 0.1 to 2.0 mg / l and the GA is 0.2 to 2.0mg / l. The invented method has the advantages of easy avail of materials and aseptic materials, low variation rate, high enhancement factor, 5 to 6 times of multiple in 4 weeks, over 93 percent of rooting rate of tissue culture seedlings, over 95 percent of survival rate of transplanting and very strong commercialized production capacity. The high productive Chinese medicine Artemisia annua is reproduced by making use of the invention, and the high arteannuin of the offspring can be stabilized, thus increasing the output of arteannuin.

The invention discloses a stable and high-efficiency method for anti-browning and tissue culture multiplication of Tokyo Sizhaohua, which comprises the following steps: 1) Taking materials: in fine weather from April to May in spring, the vegetative branches that were born in the year without disease and grew vigorously ; 2) Sterilization: The sterilization method is a combination of alcohol 30s + 0.1% mercury chloride for 7 minutes; 3) Aseptic anti-browning system establishment: the sterilized stem segments are spread out on the sterilized filter paper to dry, and inoculated in a seed containing 200mg· In the culture medium of L-1 PVP anti-browning agent, wait for the buds to grow to 3-4cm, and carry out proliferation culture; 4) Proliferation culture: inoculate the induced buds in the proliferation medium (WPM+1.0 mg·L-16- BA+0.05 mg·L-1NAA), subculture every 20-25 days. The invention optimizes each stage of tissue culture and multiplication of Tokyo Sizhaohua, effectively reduces the browning rate to 17.87%, and increases the multiplication coefficient up to 3.09.

The invention discloses a tissue culture rapid propagation method of Guangdong anoectochilus roxburghii, which selects an axillary bud or a terminal bud of anoectochilus roxburghii in the Guangdong area in China as an explant and obtains a mass of vegetative propagated progeny plants by inducing the adventitious buds, carrying out proliferation and carrying out rooting cultivation. By adopting the method of the invention, one axillary bud can be induced to generate about 2 to 4 sprouts, after two months, the proliferation coefficient is between 3 and 4, and the rooting rate can reach over 90 percent. Therefore, the method greatly improves the seedling production capacity of Guangdong anoectochilus roxburghii, shortens the seedling culture time, saves the planting cost and provides the technical guarantee for the storage of the genetic resources of Guangdong anoectochilus roxburghii.

The invention belongs to the technical field of plant tissue culture, and provides a tissue culture method using Pan's Bingdeng Yulu inflorescence rachis as explants, comprising the following steps: selecting healthy Yulu inflorescence rachis, cleaning and disinfecting, and obtaining aseptic explants Inoculating the aseptic explant into a callus-inducing medium to induce callus after cutting into sections; then cutting the callus into small pieces and transferring it to an adventitious bud differentiation medium for differentiation and cultivation to obtain clustered buds; Then, after cultivating the strong seedlings of the ramets of clustered buds, they are transferred to the rooting medium for cultivation to obtain complete plants with roots, and finally the seedlings are hardened and cultivated. The present invention obtains Yulu tissue culture seedlings by in vitro culture of Yulu inflorescence rachis tissue, the method is easy to obtain materials, is convenient for disinfection, has high callus differentiation rate, high rooting rate, ensures strain advantage, and can rapidly and massively reproduce a large number of excellent plants. Seedlings of Bingdeng Yulu.

The invention provides a method for improving regeneration efficiency in the tissue culture process of Cinnamomum camphora. First, sterilize the seeds of Cinnamomum camphora, and germinate the sterilized seeds to obtain aseptic Cinnamomum camphora seedlings; then, use the aseptic Cinnamomum camphora seedlings As explants, the corresponding Cinnamomum camphora tissue culture method is established through the steps of "induction of buds", "proliferation of buds" and "rooting of buds"; wherein, in the step of "proliferation of buds" Activated carbon was added as an additional component in the "propagation medium" used and the "rooting medium" used in the "rooting of shoots" step. The present invention has the advantages that: (1) active carbon is added in part of the culture medium to absorb metabolites, so that the value-added coefficient of tissue culture seedlings is increased, the browning rate is reduced, and the rooting rate is increased, thereby improving the regeneration efficiency of camphor tissue culture; (2) Choose to sterilize the seeds to effectively avoid the damage caused by the "disinfection process" to the young and tender stems of the materials required in subsequent experiments.

The present invention relates to a formulation of subculture medium for tissue cultured seedlings of Pearl Color Osmanthus and its application. The components of the medium include woody plant medium WPM, cytokinin 6-BA, kinetin KT and gibberellin GA 3 , including a final concentration of 6.5g·L ‑1 agar and a final concentration of 30g·L ‑1 sucrose; after analysis, the best medium for the proliferation of Zhenzhu Caigui is: woody plant medium WPM, final concentration 4.0 mg·L ‑1 Cytokinin 6-BA, final concentration 2.0 mg·L ‑1 Kinetin KT, final concentration 0.5 mg·L ‑1 gibberellin GA3, final concentration 6.5g L ‑1 Agar, final concentration 30g L ‑1 sucrose; under this condition, the multiplication coefficient of Pearl Caigui tissue culture seedlings can reach 5.8.

The invention discloses an induction method for a grape fruit callus and a culture medium thereof. The culture medium comprises the composition: a basal culture medium, 0.8-1.2 mg / L of TDZ, 0.3-0.5 mg / L of IBA, 18-22 g / L of sugar, and 5-7 g / L of agar. The induction method is characterized by comprising the steps: sterilizing grape fruits, then peeling, taking pulp blocks, inoculating the inductionculture medium with the pulp blocks, and carrying out induction culture until the callus is formed. The culture method of the fruit callus of 'Xiahei' grapes is successfully established, and the induction rate of the fruit callus reaches 100%, a new way is provided for molecular biology and genetic engineering research of the 'Xiahei' grapes, a new experimental material is provided for the genetic engineering research and gene transformation of the grapes, and meanwhile, a reference is also provided for culture of the grape fruit callus.

The invention discloses a method for improving tissue culture, proliferation and seedling expansion effects of a bamboo-root ginger. The method comprises the following steps: cluster bud induction, cluster bud subculture multiplication, tube seedling cultivation, tube seedling propagation and the like, wherein in the cluster bud induction, the subculture medium is obtained by the following steps: by taking MS as a basic culture medium, inoculating 1.5mg / L of BA into the basic culture medium, and transferring to the basic culture medium containing 1.5mg / L of BA and 0.2mg / L of NAA after 20d, wherein the concentration of NH4NO3 in the MS culture medium of the proliferation medium is 1 / 2 to 1 / 3; the cutting method of tube seedling propagation comprises the following steps: dividing a test tube cluster seedling into 2-3 adventitious buds; removing the part which is 0.5cm over the basal part of a stem; and forming micro block mass and transferring to the proliferation medium for breeding. The defects that existing bamboo-root ginger easily generates low proliferation coefficient, vitrification and yellowing in a long-term in-vitro culture process are overcome; the method for improving the tissue culture, proliferation and seedling expansion effects of the bamboo-root ginger is high in proliferation coefficient; and vitrification and yellowing are effectively reduced.

The invention discloses a peach fruit callus tissue culture medium and a culture method thereof. The culture medium comprises the following components: an improved WPM culture medium as a basic culture medium, a plant-growth regulator, 20-40 g / L of cane sugar, and 4.5-6.5 g / L of agar powder, wherein the pH value is 5.5-6.0. The culture method comprises the following steps: 1) bacteria-free treatment on peach fruits, and 2) round slice culture on the peach fruits. The callus induction rate of the peach fruits is greatly increased by the callus tissue culture medium and the culture method; a lot of high-quality callus tissues can be obtained in a short period by culturing the callus tissues of the peach pulp, problems of inconsistent callus tissue states in culture of peach tree leaves and low success rate of callus tissue culture can be solved, the method provides an effective approach for large-scale culture of the callus tissue, and provides a novel approach for molecular biology and gene engineering research of peaches.

The present invention provides a kind of rapid propagation method of schizomaea, comprising step 1: explant selection and sterilization; step 2, sterilized seeds are inoculated on the callus induction medium, and each bottle is inoculated with 10 Block explants, adjust the pH of the medium to 5.8, place them in dark culture at 25°C, and start to form callus after 5 to 7 days of culture; step 3, under dark culture conditions, the cultured on the callus medium Embryogenic callus, after peeling off, transferred to the subculture medium for culture, the pH of the medium was adjusted to 5.8, the temperature was 25±1°C, and every 20d to 28d was a multiplication cycle; step 4: bud differentiation, step 5: rooting and Proliferation; Step six: transplanting. The method for propagating the grass grass provided by the invention has the advantages of simple sampling, high induction rate of the cultured callus, high differentiation rate, high value-added coefficient, good stability, and can obtain a large amount of robust test-tube plantlets in a relatively short period of time, and The method has strong operability, low production cost and high application value, and is very suitable for industrial production.

The invention relates to the technical field of tissue culture, in particular to a medium and a method for tissue culture of euonymus maackii. The tissue culture medium provided by the invention is applicable to a variety, namely 'Jinzhi Yuye' (euonymus macckii rupr.), of the euonymus maackii; on the basis of a 1 / 2MS medium, KT, NAA, TDZ and gibberellin are added for promoting callus induction; on the basis of an MS medium, KT and TDZ are added for promoting axillary bud induction; and a WPM medium is taken as a seedling hardening medium. Induced by the medium provided by the invention, a growth coefficient can be increased to 9.72, and a survival rate of seedlings can reach 93% or above.

A lilium duchartrei franch tissue culture rapid propagation and separation preservation method comprises the steps of obtaining explant, inducing adventitious buds, carrying out enrichment culture, enlarging the bulb, carrying out rooting culture, carrying out separation preservation and carrying out transplanting. A scale of lilium duchartrei franch serves as the explant, a tissue culture rapid propagation and separation preservation system of the lilium duchartrei franch is established, the appreciation coefficient highly reaches 1:10, and the separation preservation subculture period reaches four years or longer. By means of the method, the problem of tissue culture rapid propagation of the lilium duchartrei franch is effectively solved, the separation preservation period is greatly prolonged, the preservation, the development and the utilization of the germplasm resources of the lilium duchartrei franch are facilitated, and a foundation is laid for breeding of the lilium duchartrei franch.

The invention discloses a method for inducing embryoid bodies of cabbage, which includes induction of sterile seedlings, induction of embryoid bodies, induction of regenerated plants, seedling hardening and transplantation. directly induce regeneration plants or induce embryoid bodies or induce budding, and the induction rate is not high; the following hypocotyls of the present invention are used as explants for inducing embryoid bodies, and the induction rate can reach 90%, and the embryoid bodies induce regenerated plants. The induction rate can reach 90%; the regenerated plants are induced by embryoid bodies, and the regenerated plants have high genetic stability, no physiological isolation from the mother body, and a high value-added coefficient.

The invention provides a method for breeding wenshan paphiopedilum seedlings by using somatic embryo. After mature wenshan paphiopedilum fruits are sterilized, the fruits are cut apart to obtain seeds, the seeds are inoculated in an induced germination medium to obtain young embryo, and the young embryo is inoculated in a liquid nutrient medium to obtain the somatic embryo; differentiation seedlings are obtained through differentiation cultivation; the differentiation seedlings are inoculated in a root medium to perform root cultivation, and finally the seedlings are exercised and transplanted, namely the wenshan paphiopedilum seedlings are obtained. The method for breeding wenshan paphiopedilum seedlings by using the somatic embryo obtains the young embryo after sterile germination of the wenshan paphiopedilum mature fruits, breaks dormancy of shoots, promotes germination of the seeds and growth of callus, performs rapid cultivation on the plant somatic embryo, enables wenshan paphiopedilum tissue cultivation enhancement factor to be improved to above 5 times from original 1 time, and greatly improves reproduction efficiency of the wenshan paphiopedilum. Survival rate of the wenshan paphiopedilum seedlings after transplanting reaches above 90%.