A kind of culture medium and method of silk cotton wood tissue culture

A technology of tissue culture and silk cotton wood, applied in horticultural methods, botanical equipment and methods, plant cells, etc., can solve the problem of no tissue culture medium of golden branches and jade leaves, achieve good results, and increase the effect of proliferation coefficient

Active Publication Date: 2018-07-24
山东天序农林科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is currently no medium suitable for tissue culture of golden branch and jade leaf

Method used

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  • A kind of culture medium and method of silk cotton wood tissue culture
  • A kind of culture medium and method of silk cotton wood tissue culture
  • A kind of culture medium and method of silk cotton wood tissue culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] 1. Growth environment

[0056] (1) A solid medium is used, in which the mass concentration of agar is 7g / L, and the mass concentration of sucrose is 30g / L. The prepared medium is sterilized by high-pressure high-temperature steam at a temperature of 126° C. for 5 minutes.

[0057] (2) Tissue culture room, light time 12h / d, light intensity 3500lx, culture temperature 25°C, relative humidity 60%, sterilized with ultraviolet light for 15min every day.

[0058] 2, the processing of golden branch jade leaf explant

[0059] (1) When taking, the taking time of explants is mid-April, and young and tender branches without damage by diseases and insect pests are selected.

[0060] (2) After the explants are harvested, remove the leaves.

[0061] (3) The explants were wrapped with double layers of gauze and washed under running water for 45 minutes.

[0062] (4) Finally, scrub the explants with a fine-haired brush in 0.1% NaClO solution added with several drops of Tween.

[00...

Embodiment 2

[0081] 1. Growth environment

[0082] (1) A solid medium is used, in which the mass concentration of agar is 7g / L, and the mass concentration of sucrose is 30g / L. The prepared medium is sterilized by high-pressure high-temperature steam at a temperature of 126° C. for 5 minutes.

[0083] (2) Tissue culture room, light time 12h / d, light intensity 3500lx, culture temperature 25°C, relative humidity 60%, sterilized with ultraviolet light for 15min every day.

[0084] 2, the processing of golden branch jade leaf explant

[0085](1) When taking, the taking time of explants is mid-April, and young and tender branches without damage by diseases and insect pests are selected.

[0086] (2) After the explants are harvested, remove the leaves.

[0087] (3) The explants were wrapped with double layers of gauze and washed under running water for 45 minutes.

[0088] (4) Finally, scrub the explants with a fine-haired brush in 0.1% NaClO solution added with several drops of Tween.

[008...

Embodiment 3

[0107] 1. Growth environment

[0108] (1) A solid medium is used, in which the mass concentration of agar is 7g / L and the mass concentration of sucrose is 30g / L. The prepared medium is sterilized by high-pressure high-temperature steam at a temperature of 126° C. for 5 minutes.

[0109] (2) Tissue culture room, light time 12h / d, light intensity 3500lx, culture temperature 25°C, relative humidity 60%, sterilized with ultraviolet light for 15min every day.

[0110] 2, the processing of golden branch jade leaf explant

[0111] (1) When taking, the taking time of explants is mid-April, and young and tender branches without damage by diseases and insect pests are selected.

[0112] (2) After the explants are harvested, remove the leaves.

[0113] (3) The explants were wrapped with double layers of gauze and washed under running water for 45 minutes.

[0114] (4) Finally, scrub the explants with a fine-haired brush in 0.1% NaClO solution added with several drops of Tween.

[011...

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Abstract

The invention relates to the technical field of tissue culture, in particular to a medium and a method for tissue culture of euonymus maackii. The tissue culture medium provided by the invention is applicable to a variety, namely 'Jinzhi Yuye' (euonymus macckii rupr.), of the euonymus maackii; on the basis of a 1 / 2MS medium, KT, NAA, TDZ and gibberellin are added for promoting callus induction; on the basis of an MS medium, KT and TDZ are added for promoting axillary bud induction; and a WPM medium is taken as a seedling hardening medium. Induced by the medium provided by the invention, a growth coefficient can be increased to 9.72, and a survival rate of seedlings can reach 93% or above.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a culture medium and method for silk cotton wood tissue culture. Background technique [0002] Jinzhiyuye (Euonymus maackii Rupr.'Jinzhi Yuye') is a mutant variety discovered by Zhang Jiaxun and Zhang Dan in 2003 in irradiated silk cotton wood (Euonymus maackii) seedlings. After 3 years of purification and testing Through planting, improvement and observation, the traits of the variant varieties are now stable. The main characters are: the branches and leaves are golden yellow in spring, summer and autumn, the branches and leaves turn red after frost, and the branches become brighter red after falling leaves, and the colder the weather, the redder the color. Other habits are consistent with common silk cotton wood. Light-loving, slightly shade-tolerant; cold-tolerant, not strict on soil, resistant to drought, but also resistant to water and humidity, and grows best in fe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001A01H4/008
Inventor 张丹张茂孟伟芳何甜甜王东超
Owner 山东天序农林科技有限公司
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