A kind of rapid propagation method of schistos

A technology of maize and seeds, which is applied in the field of rapid propagation of maize, can solve the problems of failure of callus induction, increase of production cost, high sampling requirements, etc., and achieve the effect of strong operability, low production cost and high induction rate

Inactive Publication Date: 2017-10-27
SICHUAN ACAD OF GRASSLAND SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

But, the defective that this cultivation method exists is:
[0010] This scheme has high sampling requirements and is difficult to operate. Inadequate sampling can easily lead to callus induction failure and increase production costs.

Method used

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  • A kind of rapid propagation method of schistos
  • A kind of rapid propagation method of schistos
  • A kind of rapid propagation method of schistos

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Embodiment Construction

[0044] In order to make the purpose, technical solutions and advantages of the present invention clearer, the following technical solutions in the present invention are clearly and completely described. Obviously, the described embodiments are some embodiments of the present invention, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0045] Such as Figure 1-10 Shown, the present invention has provided a kind of breeding method of spot grass, comprises the steps:

[0046] Step 1: Explant selection and sterilization

[0047] The seeds were used as explants, and the collected seeds were selected with full grains, peeled off with tweezers, and soaked in sterile water for 12 hours for later use. Disinfection treatment is soaking in 70% alcohol for 30 seconds, rinsing with sterile water for 3-5 ...

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Abstract

The present invention provides a kind of rapid propagation method of schizomaea, comprising step 1: explant selection and sterilization; step 2, sterilized seeds are inoculated on the callus induction medium, and each bottle is inoculated with 10 Block explants, adjust the pH of the medium to 5.8, place them in dark culture at 25°C, and start to form callus after 5 to 7 days of culture; step 3, under dark culture conditions, the cultured on the callus medium Embryogenic callus, after peeling off, transferred to the subculture medium for culture, the pH of the medium was adjusted to 5.8, the temperature was 25±1°C, and every 20d to 28d was a multiplication cycle; step 4: bud differentiation, step 5: rooting and Proliferation; Step six: transplanting. The method for propagating the grass grass provided by the invention has the advantages of simple sampling, high induction rate of the cultured callus, high differentiation rate, high value-added coefficient, good stability, and can obtain a large amount of robust test-tube plantlets in a relatively short period of time, and The method has strong operability, low production cost and high application value, and is very suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for rapid propagation of sarcophagus. Background technique [0002] Saccharum arundinaceum (Retz) Jeswiet. is a perennial herb of the genus Saccharum in the family Poaceae (Poaceae) and the genus Saccharum. It is more than 4 meters high, even 6-7 meters high, and the stem is about 2 cm thick. Widely distributed in subtropical to warm temperate regions, India, Myanmar, Thailand, Vietnam, Malaysia and other countries also have abundant wild resources. Because of its excellent botanical characteristics, it has been widely used in the genetic improvement of cultivated sugarcane, through distant hybridization, gene introduction and other means to make the plants tall, strong stress resistance, more tillers, good perennial roots, etc. Excellent traits have been transferred to sugarcane, and a large number of new varieties of excellent cultivated sugarcane have been...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 鄢家俊白史且张瑜张建波张劲游明鸿李达旭
Owner SICHUAN ACAD OF GRASSLAND SCI
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