A kind of rapid propagation method of schistos

Abstract

The present invention provides a kind of rapid propagation method of schizomaea, comprising step 1: explant selection and sterilization; step 2, sterilized seeds are inoculated on the callus induction medium, and each bottle is inoculated with 10 Block explants, adjust the pH of the medium to 5.8, place them in dark culture at 25°C, and start to form callus after 5 to 7 days of culture; step 3, under dark culture conditions, the cultured on the callus medium Embryogenic callus, after peeling off, transferred to the subculture medium for culture, the pH of the medium was adjusted to 5.8, the temperature was 25±1°C, and every 20d to 28d was a multiplication cycle; step 4: bud differentiation, step 5: rooting and Proliferation; Step six: transplanting. The method for propagating the grass grass provided by the invention has the advantages of simple sampling, high induction rate of the cultured callus, high differentiation rate, high value-added coefficient, good stability, and can obtain a large amount of robust test-tube plantlets in a relatively short period of time, and The method has strong operability, low production cost and high application value, and is very suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for rapid propagation of sarcophagus. Background technique [0002] Saccharum arundinaceum (Retz) Jeswiet. is a perennial herb of the genus Saccharum in the family Poaceae (Poaceae) and the genus Saccharum. It is more than 4 meters high, even 6-7 meters high, and the stem is about 2 cm thick. Widely distributed in subtropical to warm temperate regions, India, Myanmar, Thailand, Vietnam, Malaysia and other countries also have abundant wild resources. Because of its excellent botanical characteristics, it has been widely used in the genetic improvement of cultivated sugarcane, through distant hybridization, gene introduction and other means to make the plants tall, strong stress resistance, more tillers, good perennial roots, etc. Excellent traits have been transferred to sugarcane, and a large number of new varieties of excellent cultivated sugarcane have been...

AI Technical Summary

Problems solved by technology

But, the defective that this cultivation method exists is:

[0010] This scheme has high sampling requirements and is difficult to operate. Inadequate sampling can easily lead to callus induction failure and increase production costs.

Images