39 results about "Saccharum arundinaceum" patented technology

The invention relates to an agaricus blazei murill cultivation base material which is prepared from the following raw materials in parts by weight: 50-60 of bagasse, 10-15 of saccharum arundinaceum powder, 20-30 of watermelon vine particles, 10-13 of eucalyptus wood powder, 15-18 of tea oil bran, 15-16 of potato dregs, 2-3 of peanut shells, 5-8 of shrimp shell meal, 2-3 of quick lime, 1-2 of gypsum powder, 4-6 of plant ash and 5-10 of slow-release nutrition particles. The cultivation substrate has the advantages of wide material sources, rich resource and low cost, and the low-release nutrition particles are added in the cultivation material. The obtained agaricus blazei murill has a high biotransformation rate and richer nutrition, and meanwhile a qi-tonifying health care efficacy is increased. The agaricus blazei murill has the qi-tonifying health-care efficacy after long-term eating and has high yield.

In the nature, dictyophora indusiata grows on bamboo humus, and nutrition is obtained in a way that rotten bamboo organic matters are decomposed by mycelia. Bamboo rats also known as Mangli, Zhuli and the like belong to mammalia rodentia rhizomyidae rhizomys, and are named because staple food is bamboo. The bamboo rats are rich in nutritional value and high in economic effect, and are bred in a large scale at present. At present, a crude feed for artificially feeding the bamboo rats mainly comprises bamboo (accounting for 50% of the feed), other crude feeds comprise neyraudia reynaudiana, saccharum arundinaceum, miscanthus floridulus, pennisetum purpureum and pennisetum sinese roxb seeds, and a fine feed is starch grains (accounting for 10% of the feed). The feeds are digested to form feces through bamboo rat unique digestive tracts and digestive enzymes. The bamboo rat feces contains cellulose, hemicellulose and lignin obtained from microorganism and enzyme decomposition and conversion, and also contains a certain amount of proteins, starch, wax, fat and resin. The feces also contains amino acids, various effective trace elements, various vitamins, saccharides, carbohydrates and the like, and is rich in nutrition. The bamboo rat feces can be supplied for dictyophora indusiata growth without stacking and fermentation, and is an important cultivation base for formation of high-quality dictyophora indusiata.

The invention provides SSR primers, applications of the SSR primers in detection of polymorphism of saccharum arundinaceum, a design method of the SSR primers, and a method used for detecting the polymorphism of saccharum arundinaceum by using the SSR primers. The detection method is capable of detecting some genomic SSR locus information of saccharum arundinaceum; in addition, according to verification of polymorphism of saccharum arundinaceum by using the designed primers, the designed primers can be used for the research on genetic diversity of saccharum arundinaceum and construction of genetic maps effectively; an advanced gene analyzer is used in the development of the polymorphism, so that accuracy of primer polymorphism detection is increased.

The invention mainly belongs to the field of cultivation of an edible / medicinal fungi by utilizing Juncao in the fungus production and particularly relates to a method for cultivating the edible / medicinal fungi by utilizing the Juncao. A culture medium is prepared from the steps of taking dried Juncao as raw materials, adding urea, lime and a metal inorganic salt preparation, mixing the materials and performing composting fermentation, and the culture medium of the edible / medicinal fungi is prepared. The urea is added to control the carbon nitrogen ratio of the culture medium of the edible / medicinal fungi to be in a range of (30-40):1. The Juncao is one or any compositions of neyraudia reynaudiana, miscanthus floridulus, imperatorial, reed, chrysopogon zizanioides and saccharum arundinaceum. According to the method, the urea is added to adjust the carbon nitrogen ratio and enable the carbon nitrogen ratio to be (30-40):1. The carbon nitrogen ratio in the range is the best carbon nitrogen ratio needed by the edible / medicinal fungus entity in the growth and development period. The metal inorganic salt preparation is added in the method to meet the requirements of the edible / medicinal fungus for metallic elements.

The invention relates to a method for identifying consanguinity of saccharum arundinaceum in sugarcane via a spread sequence. The method comprises steps of preparing a root-tip cell slide, preparing alabeled probe, pre-processing the root-tip slide, preparing a denaturing hybrid mixed solution and hybridizing the denaturing hybrid mixed solution and the pre-processed root-tip slide. A to-be-detected sample is judged that the sample contains the consanguinity of saccharum arundinaceum and is a real hybrid later generation of sugarcane and saccharum arundinaceum when a green fluorescence signalis generated on the hybrid slide. The nucleotide sequence of a probe which generates the green fluorescence signal is shown as SEQ ID NO:1 in a sequence list. The method provided by the invention, through the combination with fluorescence in-situ hybridization technology and by identifying the real hybrid later generation of sugarcane and saccharum arundinaceum through the spread sequence probe that saccharum arundinaceum is specific to sugarcane, has the characteristics of being high in efficiency, good in sensitivity, good in stability and high in reliability; and the reality of any hybridgenerations can be detected just by acquiring root tips of sugarcane seedlings, so that an efficient and stable identifying method is provided for hybridization utilization of the saccharum arundinaceum in a sugarcane genetic breeding improving process.

The invention provides a preparation method of a Saccharum arundinaceum root-tip meristematic zone chromosome specimen, comprising: root-tip culture, root-tip sampling and pretreatment, root-tip enzymolysis, chromosome preparation, microscopic examination and other steps; the preparation method is characterized in that a whole-plant culture root system is planted in a bucket, the root tip is treated with 0.002 M of 8-hydroxyquinoline solution at 25-28 DEG C for 3-4 h, and the specimen is prepared by an enzymolysis wall degradation hypotonic method. The preparation method is highly operable, allows high-active meristematic zone root tip to be easily obtained, takes shorter time for root-tip culture, is low in manpower and material resource consumption and high in preparation efficiency; many interphases of meristematic zone cells are obtained; the obtained chromosome has vivid form and good dispersion uniformity, and is suitable particularly for the preparation of Saccharum arundinaceum chromosome specimens; the preparation method is also applicable to the preparation of chromosome specimens of sugarcane and plants in its related genera.

The invention belongs to the technical field of medicine and particularly relates to traditional Chinese medicine for treating perennial allergic rhinitis. The traditional Chinese medicine is composed of, by weight, 11-19 parts of Chinese clematis, 6-14 parts of clerodendrum bungei roots, 5-13 parts of spuriopimpinella brachycarpa roots, 6-14 parts of ardisia brevicaulis diels roots, 5-13 parts of calamus, 5-13 parts of sambucus chinensis, 2-4 parts of saccharum arundinaceum and 4-8 parts of stichopus japonicus. By means of the traditional Chinese medicine, the perennial allergic rhinitis can be treated, the curative efficiency is as high as 58%, the total effective rate is 94%, and no adverse reaction occurs.

The invention belongs to the field of genetic breeding. The invention particularly relates to an SSR molecular marker and method for identifying authenticity of filial generations of sugarcane and saccharum arundinaceum. The SaSSR molecular marker is amplified by adopting 10 primer pairs with nucleotide sequences as shown in SEQ ID NO: 1-20, an amplification product is detected by agarose gel electrophoresis, and the authenticity of the filial generation of sugarcane and saccharum arundinaceum is identified by analyzing strips. The SaSSR molecular marker disclosed by the invention is a group of specific SSR markers with different amplification bands between sugarcane and saccharum arundinaceum, uniform distribution of the markers in different chromosomes of saccharum arundinaceum is ensured, and the SSR molecular marker and method are particularly suitable for identification of sugarcane and saccharum arundinaceum high-generation materials.

The invention belongs to the technical field of agriculture and particularly relates to a pollution-free insecticide and a preparation method thereof. The pollution-free insecticide comprises datura stramonium, tripterygium wilfordii hook, anisodus acutangulus, zanthoxylum bungeanum maxim, coriaria sinica, saccharum arundinaceum, verdigris, venenum bufonis and sulfur. The pollution-free insecticide comprises the components in parts by mass: 20-37 parts of the datura stramonium, 16-29 parts of the tripterygium wilfordii hook, 8-17 parts of the anisodus acutangulus, 24-42 parts of the zanthoxylum bungeanum maxim, 3-18 parts of the coriaria sinica, 12-28 parts of the saccharum arundinaceum, 2-12 parts of the verdigris, 3-14 parts of the venenum bufonis and 6-17 parts of the sulfur. The pollution-free insecticide provided by the invention has the advantages that synthesized components which are commonly used in the existing insecticide are not added and the pollution-free insecticide is prepared by adopting a full traditional Chinese medicine formula so that great damages to contact crowd or the environment are not caused except that the final insecticide meets the insecticidal requirements.

The invention relates to a method for performing genome in-situ hybridization in a chromosome suspension (Genome in situ hybridization in suspension, GISHIS). The method comprises the steps of cell cycle synchronization, preparation of the chromosome suspension, preparation of a genomic probe, denaturation of samples and genomic probe and genome in-situ hybridization in the suspension. Dripping piece microscopic examination is carried out, if a green fluorescence signal is generated in the suspension, a saccharum arundinaceum chromosome is determined, so that it is indicated that green fluorescence is successfully marked onto the saccharum arundinaceum chromosome. A flow cytometry is utilized to achieve the separation of the saccharum arundinaceum chromosome specially marked with the greenfluorescence signal in the chromosome suspension and perform the related sequencing study, thereby providing a new, effective and feasible method for studying a complex polyploid saccharum arundinaceum genome. The method is applied to the genome in-situ hybridization in the chromosome suspension of intergeneric hybridization offspring in various crops.

The invention relates to a freckle removing capsule. The freckle removing capsule is prepared from saccharum arundinaceum, pearl powder, mint, honey, rhodiola rosea, climbing groundsel herb, aloes, sweet wormwood herb, radix angelicae, fructus lycii and cornu cervi pantotrichum. The freckle removing capsule is low in cost, a preparation method of the freckle removing capsule is simple, and the freckle removing capsule is capable of beautifying, removing freckles, improving detoxifying action of human bodies and enhancing immunity.

The invention discloses a method for screening drought-resistant variant sugarcane plants, belongs to the technical field of molecular breeding of sugarcane, and particularly relates to a selection breeding method for the drought-resistant sugarcane plants. The selection breeding method for the drought-resistant sugarcane plants comprises the steps that co-culturing is performed on small embryogenic suspension sugarcane cells through the appropriate saccharum arundinaceum total DNA solution concentration, cultures are cultured on MS culture media with the different PEG concentrations, repeated drought-resistant screening is performed on proliferation, differentiation and the like of calluses, SRAP molecular detection is performed on the variant plants, and then the drought-resistant sugarcane plants are finally selected and bred through drought-resistant stress selection in a greenhouse. According to the method, the sugarcane selection breeding time is shortened, operation is easy, and the cost is low; meanwhile, a novel path capable of breaking through close hybridization of sugarcane genera and species is supplied, and the obtained sugarcane plants have the better drought resistance than a control group.

The invention discloses ointment for treating skin allergy. The ointment comprises, by weight, 5-15 parts of remote lemongrass herb, 10-20 parts of saccharum arundinaceum, 5-15 parts of centipedes, 15-25 parts of musk, 5-15 parts of eucalyptus leaves, 15-25 parts of rice vinegar, 10-20 parts of salicylic acid and 15-25 parts of climbing groundsel herb. The ointment has the advantages that the ointment is prescribed according to traditional Chinese medicine theories, effects of clearing heat, removing toxicity, reducing swelling, resolving mass, removing heat to cool blood and relieving pain and itching can be realized by the ointment, a method for preparing the ointment is simple, and obvious curative effects can be realized.

The invention belongs to the technical field of medicaments, and particularly relates to a traditional Chinese medicine composition for treating kidney deficiency, liver depression and dampness-heat type tubal infertility. The traditional Chinese medicine composition is prepared from the following traditional Chinese medicinal materials in parts by weight: 5 to 13 parts of rubus hirsutus, 5 to 13 parts of Gymnadenia conopsea, 4 to 8 parts of Akebia trifoliata, 5 to 13 parts of Lathyrus davidii, 4 to 8 parts of Carex canescens, 5 to 13 parts of Hemiphragma heterophyllum, 8 to 16 parts of Crepis rigescens, 5 to 13 parts of Herminium monorchis, 4 to 8 parts of kumquat, 5 to 13 parts of rosa rugosa, 5 to 13 parts of Jordan almonds, 8 to 16 parts of cucubalus root, 8 to 16 parts of Saccharum arundinaceum, 4 to 8 parts of Euonymus frigidus, 4 to 8 parts of Sinkiang Euonymus alatus, 8 to 16 parts of Myriopteron extensum, 8 to 16 parts of Anthriscus sylvestris and 4 to 8 parts of glycyrrhiza uralensis. The traditional Chinese medicine composition has the effects of tonifying kidney, smoothing liver-qi stagnation, activating blood to regulate menstrual flow and promoting gestation by combined use of all the medicinal materials, focuses on the etiology and pathogenesis of the kidney efficiency, liver depression and blood stasis type tubal infertility, and can be used for effectively improving the syndromes of Chinese medicine and improving the intrauterine pregnancy rate.

The invention belongs to the technical field of medicaments, and particularly relates to a traditional Chinese medicine composition for treating kidney deficiency and blood stasis type tubal infertility. The traditional Chinese medicine composition is prepared from the following traditional Chinese medicinal materials in parts by weight: 4 to 8 parts of Akebia trifoliata, 5 to 13 parts of Lathyrus davidii, 4 to 8 parts of Carex canescens, 5 to 13 parts of Hemiphragma heterophyllum, 4 to 8 parts of kumquat, 5 to 13 parts of Rosa rugosa, 5 to 13 parts of Jordan almonds, 8 to 16 parts of cucubalus root, 8 to 16 parts of Saccharum arundinaceum, 4 to 8 parts of Euonymus frigidus, 4 to 8 parts of Sinkiang Euonymus alatus, 8 to 16 parts of Myriopteron extensum, 8 to 16 parts of Anthriscus sylvestris and 4 to 8 parts of glycyrrhiza uralensis. The traditional Chinese medicine composition has the effects of soothing liver-qi stagnation, activating blood to regulate menstrual flow and promoting gestation by combined use of all the medicinal materials, focuses on the etiology and pathogenesis of the kidney efficiency, phlegmatic hygrosis and blood stasis type tubal infertility, and can be used for effectively improving the syndromes of Chinese medicine and improving the intrauterine pregnancy rate.

The invention discloses rhizomyidae manure as a main fertilizer of rosmarinus officinalis fertilizers, wherein, the breeding manner of rhizomyidae does not employ antibiotics. Forages of rhizomyidae mainly comprise bamboo root, bamboo stem and bamboo leaf. Because bamboo is limit in nutrition, rhizomyidae need to ingest a large amount of bamboo roots, bamboo stem and bamboo leaves every day, and a large amount of neyraudia reynaudiana, saccharum arundinaceum, miscanthus floridulus, pennisetum purpureum and pennisetum sinese seed are added for artificial breeding, and the forages are natural, organic and free of pesticide residues, heavy metal pollution and the like. Rhizomyidae manure contains a certain amount of protein, starch, wax, fat and resin besides cellulose obtained through decomposition conversion of microbes and enzymes, hemicellulose and lignin. The manure also contains amino acids, multiple effective trace elements, multiple vitamins, saccharides, carbohydrates and the like, and is abundant in nutrition. A fertilizer is prepared by mixing 75% of rhizomyidae manure with a dictyophora indusiata cultivation material disclosed in a patent with the application number of 201310225040.1, and the fertilizer is beneficial for improving soil, increasing the content of rosmanol, carnosol and carnosic acid in rosmarinus officinalis, increasing the germination rate of a seed, and enabling the plant to grow flourishingly and branches and leaves to be dense.

The invention discloses application of a Chinese herbal preparation to treatment of chronic renal failure. The Chinese herbal preparation comprises the following raw materials containing effective constituents by weight: 60 to 90 parts of herba elsholtziae ciliatae, 57 to 87 parts of radix paeoniae alba, 55 to 85 parts of radix morindae officinalis, 53 to 83 parts of saccharum arundinaceum, 50 to 80 parts of limonium sinense, 45 to 75 parts of salvia miltiorrhiza, 42 to 72 parts of herb of thymeleaf speed welt, 40 to 70 parts of rosa multiflora Thunb., 38 to 68 parts of lysimachia paridiformais Franch., 35 to 65 parts of herb of Japanese conehead, 33 to 63 parts of carthamus tinctorius, 31 to 61 parts of root and rhizome of bloodred iris, 30 to 60 parts of polygonum runcinatum, 26 to 56 parts of fiveleaf akebia fruit, 25 to 55 parts of rhizome of elderleaf rodgersflower, and 20 to 50 parts of aleuritopteris argentea. The Chinese herbal preparation has no toxic or side effect, is obvious in renal failure treating effect, low in cost, fast in effect taking and lasting in curative effect, and has no adverse reaction.

The invention provides a screening method of a saccharum arundinaceum breeding material. The method comprises the following steps: collecting saccharum arundinaceum resources, and performing variation analysis of phenotypic character and correlation analysis among characters on the resources; extracting the genome DNA of saccharum arundinaceum as a template, adopting SCoT labeled primers to perform amplification, detecting amplified products through polyacrylamide electrophoresis, and obtaining a DNA finger-print chromatogram; performing polymorphic analysis and J genetic similarity coefficient analysis on the saccharum arundinaceum resources, and constructing UPGMA phylogenetic trees; performing comprehensive analysis on different ecological positions, phenotypic variation characteristics and phylogenetic trees of different saccharum arundinaceum resources, so as to obtain the screened material. By adopting the technical scheme and in combination with the phenotypic character variation analysis and a molecular marker technology, screening results are more sensitive and accurate; the utilization potential of saccharum arundinaceum germplasm resources is comprehensively evaluated, the accharum arundinaceum breeding material is screened, and the method can serve cane breeding.

The invention provides a screening method of a saccharum arundinaceum breeding material. The method comprises the following steps: collecting saccharum arundinaceum resources, and performing variation analysis of phenotypic character and correlation analysis among characters on the resources; extracting the genome DNA of saccharum arundinaceum as a template, adopting SCoT labeled primers to perform amplification, detecting amplified products through polyacrylamide electrophoresis, and obtaining a DNA finger-print chromatogram; performing polymorphic analysis and J genetic similarity coefficient analysis on the saccharum arundinaceum resources, and constructing UPGMA phylogenetic trees; performing comprehensive analysis on different ecological positions, phenotypic variation characteristics and phylogenetic trees of different saccharum arundinaceum resources, so as to obtain the screened material. By adopting the technical scheme and in combination with the phenotypic character variation analysis and a molecular marker technology, screening results are more sensitive and accurate; the utilization potential of saccharum arundinaceum germplasm resources is comprehensively evaluated, the accharum arundinaceum breeding material is screened, and the method can serve cane breeding.

The invention discloses ointment for treating bromhidrosis and a preparation method thereof. The ointment is prepared from the following components in parts by weight: 6 to 10 parts of plantain herb, 10 to 18 parts of lemon peel, 3 to 6 parts of fructus lycii, 4 to 7 parts of fructus gardeniae, 3 to 5 parts of liquorice root, 6 to 12 parts of lithargite, 2 to 4 parts of radix scrophulariae, 6 to 10 parts of rhizoma corydalis, 2 to 4 parts of rhizoma anemarrhenae, 10 to 15 parts of stem of common perilla, 7 to 13 parts of herba pogostemonis, 4 to 8 parts of radix notoginseng, 7 to 15 parts of herba menthae and 1 to 2 parts of saccharum arundinaceum root. According to the ointment disclosed by the invention, a prescription composition is scientific and simple; all crude drugs have a synergistic effect to realize the effects of clearing away heat and detoxifying, and regulating qi and removing odor; the ointment has a rapid effect, can be used for treating both principal and secondary aspects of diseases and has no toxic and side effects. Furthermore, the formula does not contain any additive, has no harms to human bodies and is green and environmentally friendly.

The invention provides SSR primers, applications of the SSR primers in detection of polymorphism of saccharum arundinaceum, a design method of the SSR primers, and a method used for detecting the polymorphism of saccharum arundinaceum by using the SSR primers. The detection method is capable of detecting some genomic SSR locus information of saccharum arundinaceum; in addition, according to verification of polymorphism of saccharum arundinaceum by using the designed primers, the designed primers can be used for the research on genetic diversity of saccharum arundinaceum and construction of genetic maps effectively; an advanced gene analyzer is used in the development of the polymorphism, so that accuracy of primer polymorphism detection is increased.

The invention provides bleeding-stopping and inflammation-diminishing spray for slight injuries and a preparation method of the bleeding-stopping and inflammation-diminishing spray. The preparation method comprises the following steps: (1) preparing radix cynoglossi amabilis, herb of saxifraga punetata, catalpa leaf and saccharum arundinaceum inflorescence extract; (2) preparing pleurotus albellus, rattan and root and branch of euonymus porphyreus extract; (3) preparing murdannia simplex herb, root-bark of roundleaf tallowtree and oenanthe stolonifera herb extract; (4) mixing the extract prepared by the steps (1), (2) and (3); adding collagen, borneol, menthol crystal, water and ethanol, and stirring until all the materials are uniformly mixed. The bleeding-stopping and inflammation-diminishing spray has obvious bleeding-stopping and inflammation-diminishing pharmacological effect on the slight injuries; the spray is clarified and transparent and does not contain insoluble impurities; the spray is sprayed into mist and is safe and reliable; the spray can be used as an ideal drug for the slight injuries.

The invention relates to a Sedum middendorffianum Maxim detoxicating and sore curing tea and a preparation method thereof. The Sedum middendorffianum Maxim detoxicating and sore curing tea is prepared from tea and a Chinese herb extract in a mass ratio of 1:4. The Chinese herb extract includes the following raw materials by weight percentage: 20-40% of Sedum middendorffianum Maxim, 10-20% of Viburnum hainanense root and leaf, 10-15% of Euonymus fortune, 10-15% of Borreria stricta, 5-10% of Damnacanthus indicus, 5-10% of Saccharum arundinaceum, 5-10% of madder, 3-5% of Herba Elsholtziae Ciliatae, 3-5% of Miscanthus floridulus, and 3-5% of rhubarb. The preparation method includes the main steps of: a. material preparation; b. filtrate extraction; c. centrifugal separation and concentration; d. daubing and drying; and e. roasting and sterilization. After long-term drinking, the Sedum middendorffianum Maxim detoxicating and sore curing tea provided by the invention has the efficacy of removing toxin and curing sores, stopping bleeding and diminishing swelling, and strengthening and consolidating body resistance.

The invention provides a fast propagation method for saccharum arundinaceum. The fast propagation method comprises the following steps: I, selecting explants and sterilizing the explants; II, sterilizing treated seeds, inoculating the sterilized seeds on a callus-inducing medium, inoculating ten explants in each culture flask, and adjusting the pH value of the culture medium to be 5.8, and carrying out dark cultivation at 25 DEG C, wherein calluses are formed after 5 to 7 days' cultivation; III, under the dark cultivation condition, peeling off embryonic calluses which are cultivated on the callus culture medium, transferring the embryonic calluses onto a subculture culture medium for cultivation, and adjusting the pH value of the subculture culture medium to 5.8, wherein the temperature is 24 to 26 DEG C, and each proliferation cycle lasts for 20 to 28 days; IV, differentiating buds; V, rooting and proliferating; VI, transplanting. The fast propagation method provided by the invention has the advantages that the sampling is simple and convenient; the inductivity of a cultivated callus is high; the differentiation rate, the proliferation coefficient and the stability are high; a large quantity of tube seedlings which grow healthily can be obtained in a relative short time. The fast propagation method has the advantages that the maneuverability is high; the production cost is low; the application value is high; the suitability for industrialized production is very high.

The invention discloses a traditional Chinese medicine for treating chronic nephritis. The traditional Chinese medicine preparation is prepared from the following raw materials as effective ingredients in parts by weight: 60-90 parts of cogongrass rhizome, 57-87 parts of cordyceps sinensis, 55-85 parts of morinda root, 53-83 parts of saccharum arundinaceum, 50-80 parts of limonium suffruticosum, 45-75 parts of red sage root, 42-72 parts of thymeleaf speedwell herb, 40-70 parts of multiflora rose fruit, 38-68 parts of chloranthus holostegius, 35-65 parts of Japanese conehead herb, 33-63 parts of safflower, 31-61 parts of bloodred iris rhizoma and root, 30-60 parts of polygonum runcinatum, 26-56 parts of fiveleaf akebia fruit, 25-55 parts of featherleaf rodgersflower and 20-50 parts of aleuritopteris argentea. The traditional Chinese medicine disclosed by the invention is free of toxic or side effect, capable of obviously improving renal failure, low in cost, high in effect taking speed, lasting in curative effect and free of adverse reaction.

The invention discloses a method for removing and treating perennial intractable weed saccharum arundinaceum, which comprises the following steps: step 100, before germination of saccharum arundinaceum in spring or vigorous growth period of saccharum arundinaceum in summer, cutting and removing saccharum arundinaceum from stem bases; step 200, after saccharum arundinaceum germinates new stems and leaves, adopting chemical agents for spraying prevention and treatment. The method has the technical effects that the intractable saccharum arundinaceum weeds can be eradicated in the current year when the method is used for preventing and controlling the intractable saccharum arundinaceum weeds, and reoccurrence is avoided; the method is convenient in medicament source, simple to operate and easy to construct, can be popularized and applied in large-area difficult site afforestation of the saccharum arundinaceum, and promotes the greening process of barren mountains and wastelands.

The invention relates to a method for identifying consanguinity of saccharum arundinaceum in sugarcane via a spread sequence. The method comprises steps of preparing a root-tip cell slide, preparing alabeled probe, pre-processing the root-tip slide, preparing a denaturing hybrid mixed solution and hybridizing the denaturing hybrid mixed solution and the pre-processed root-tip slide. A to-be-detected sample is judged that the sample contains the consanguinity of saccharum arundinaceum and is a real hybrid later generation of sugarcane and saccharum arundinaceum when a green fluorescence signalis generated on the hybrid slide. The nucleotide sequence of a probe which generates the green fluorescence signal is shown as SEQ ID NO:1 in a sequence list. The method provided by the invention, through the combination with fluorescence in-situ hybridization technology and by identifying the real hybrid later generation of sugarcane and saccharum arundinaceum through the spread sequence probe that saccharum arundinaceum is specific to sugarcane, has the characteristics of being high in efficiency, good in sensitivity, good in stability and high in reliability; and the reality of any hybridgenerations can be detected just by acquiring root tips of sugarcane seedlings, so that an efficient and stable identifying method is provided for hybridization utilization of the saccharum arundinaceum in a sugarcane genetic breeding improving process.

The present invention provides a kind of rapid propagation method of schizomaea, comprising step 1: explant selection and sterilization; step 2, sterilized seeds are inoculated on the callus induction medium, and each bottle is inoculated with 10 Block explants, adjust the pH of the medium to 5.8, place them in dark culture at 25°C, and start to form callus after 5 to 7 days of culture; step 3, under dark culture conditions, the cultured on the callus medium Embryogenic callus, after peeling off, transferred to the subculture medium for culture, the pH of the medium was adjusted to 5.8, the temperature was 25±1°C, and every 20d to 28d was a multiplication cycle; step 4: bud differentiation, step 5: rooting and Proliferation; Step six: transplanting. The method for propagating the grass grass provided by the invention has the advantages of simple sampling, high induction rate of the cultured callus, high differentiation rate, high value-added coefficient, good stability, and can obtain a large amount of robust test-tube plantlets in a relatively short period of time, and The method has strong operability, low production cost and high application value, and is very suitable for industrial production.