32results about How to "Sensitive result" patented technology

The invention discloses a detachable pressure sore prevention nursing mattress. The problems in the prior art that a mattress for preventing pressure sores is single in function and inconvenient to maintain and clean are solved. The detachable pressure sore prevention nursing mattress disclosed by the invention comprises a mattress body and a mattress cover, wherein inflatable bags in detachable connection with the surface of the mattress body are arranged on the mattress body; more than two inflatable bags are distributed in an array mode, and odd rows of the inflatable bags and even rows of the inflatable bags are arranged in a staggered manner; an inflation and deflation mechanism and a ventilation are arranged on the mattress body; the mattress cover mainly comprises sleeves; every two of the six sleeves are detachably connected with each other to encircle a regular hexagon hollow; more than two regular hexagon hollows form a hollow row; more than two hollow rows are arranged in parallel to form the mattress cover; and when the mattress cover covers the mattress body, each inflatable bag corresponds to one regular hexagon hollow and penetrates through the corresponding regular hexagon hollow. The detachable pressure sore prevention nursing mattress disclosed by the invention is simple in structure, capable of effectively preventing pressure sores and is convenient to clean and maintain and can effectively prevent foot drop caused by long-term bedridden.

The invention discloses a method for measuring the maximum physiological capacitance and the maximum tensity of a plant leaf, and belongs to the technical field of detection on physiological information of a plant. According to the method, in different salinity levels, a set of relationship models between the physiological capacitance of the plant leaf and the salinity level and between the tensity of the plant leaf and the salinity level are established; exponential decay equations of two parameters are used for fitting relationships between correlated electrophysiological indexes and the salinity level to obtain corresponding expressions, and according to the expressions, the maximum physiological capacitance and the maximum tensity of the plant leaf are obtained. By a physical method, the damage to the plant is small, a result is sensitive, the accuracy is high, the physiological capacitance and the tensity of the living plant can be truly reflected, and the problem that the physiological capacitance and the tensity of the plant leaf are difficultly measured directly by an instrument because a zero solute concentration state of a cell fluid does not exist in actual situation is solved.

The invention relates to an SF6 electrical equipment state detection method and simulation detection device. The invention provides a new marker CO2 for detecting an SF6 electrical equipment state, which can be used as a gas maker to be applied to SF6 electrical equipment state detection. The new gas marker CO2 is discovered by analyzing gas components of faulted SF6 electrical equipment; the inner operation state of the SF6 electrical equipment can be effectively determined by detecting the CO2 concentration of contents of the SF6 electrical equipment. Compared with a traditional detection method of detecting gas markers such as CF4, SO2, SOF2, SO2F2, etc., the detection method of the invention is simpler, and can obtain more sensitive, effective and reliable results.

The invention relates to the technical field of traditional Chinese medicine identification and particularly relates to a radix bupleuri capillary electrophoresis DNA (Deoxyribonucleic Acid) fingerprint spectrum and an identification method. The radix bupleuri capillary electrophoresis DNA fingerprint spectrum comprises extraction of DNA, PCR (Polymerase Chain Reaction) amplification, analysis of an amplified product, and establishment of the radix bupleuri capillary electrophoresis DNA fingerprint spectrum. The identification method of a radix bupleuri DNA fingerprint comprises the steps of: establishment of the radix bupleuri capillary electrophoresis DNA fingerprint spectrum, establishment of a sample DNA fingerprint spectrum and result identification by utilizing similarity software. The fingerprint spectrum and the identification method can be used for identifying a radix bupleuri sample. The fingerprint spectrum and the identification method can be used for carrying out authenticity identification on radix bupleuri from heredity essence; and the method is rapid, accurate and objective and can be used for identifying the radix bupleuri sample.

The invention discloses a design method of a probe and primer combination for digital PCR amplification, the probe and primer combination for digital PCR amplification, a digital PCR amplification method and an application thereof. The problem that a digital PCR fluorescent probe and primer design is limited by a base sequence is solved, a novel problem and primer design thought and a novel probeand primer combination are provided, a two-step method comprising purification after primary amplification and then secondary amplification is not needed, and conventional one-step operation is only required, so that the possibility that a sample is polluted is reduced, and an amplified fluorescent signal is better.

The invention discloses a serum metabolic biomarker for an acute anaphylaxis guinea pig. The biomarker is lysophosphatidylcholine matter. The serums of sensitized guinea pig and healthy guinea pig aremixed with methanol to prepare to be sample solution; a technical platform for an ultra-high efficient liquid chromatogram-four-grade rod flight time tandem mass spectrum (UPLC-QTof/MS) is applied tocollect the serum metabolic spectrum of the guinea pig, and data is imported to EZinfo software to perform model identification and analysis. By comparing the serum of the of sensitized guinea pig with that of the healthy guinea pig, the lysophosphatidylcholine type (lysoPC (16: 0), lysoPC (18: 2), lysoPC (18: 0) metabolic substance level peforamnce is obviously improved; the abnormal change of the matter can be used for judging if the guinea pig is allergic or normal.

The invention discloses a preparation method of a trichinella spiralis antigen labeled gold nanorod. The method comprises the following steps: preparing spherical gold nanoparticle seeds by reducing an HAuCl4 solution with a reducing agent; adding the spherical gold nanoparticle seeds into a growth medium, and enabling the spherical gold nanoparticle seeds to grow into a rod-shaped gold nanorod ina rod micelle solution; labeling the gold nanorod by using an antigen excreted by trichinella spiralis larvae, thereby obtaining the trichinella spiralis antigen labeled gold nanorod. The invention further provides a trichinella spiralis antigen labeled gold nanorod prepared by the method. The gold nanorod is synthesized by selecting a 'seed mediated growth method', and the length-width ratio hasobvious advantages compared with a spherical nanorod; a gold nanorod labeling technology can be applied to serological test of trichinella spiralis, the detection kit is high in detected antibody dilution, early in time and low in infection gradient compared with a mouse serum antibody ELISA (Enzyme-linked Immuno Sorbent Assay) detection kit, and a novel technology can be provided for detecting early trichinosis infection and low infection gradient.

The invention discloses a kit used for evaluating ovarian cancer primary chemotherapeutic sensitivity, and an application thereof. The kit comprises a blocking reagent, an antigen-antibody reaction system, an avidin-oxide enzyme reaction system and a counterstaining drug. The invention is provided based on the relevance between the expression level of ERCC2 in serous ovarian cancer cells/tissues and sensitivity of cells, tissues or a tumor patient as a host to a primary chemotherapeutic drug. Through ERCC2 detection, purposes such as chemotherapeutic sensitivity/drug resistance evaluation, tumor patient discrimination, and primary chemotherapeutic drug tolerability evaluation can be achieved. The method provided by the invention is simple and accurate. The requirement on a sample is not high. Therefore, the kit and the method can be used in large-scale screening or trace researches. When the kit and the method is used in clinical diagnosis and medication guidance, ex vivo cancer tissues of a patient can be acquired for detection after operation, and the detection is characterized in no wound, no pain, and noninvasiveness. The detection can easily be accepted by patients. With the kit and the application, patient lives can be prolonged, chemotherapy pains of the patients can be alleviated, and life qualities of the patients after operations can be improved.

The invention relates to the technical field of organic small molecule isotope labeling, in particular to a stable isotope mercapto compound labeling reagent, a synthesis method and application thereof. The isotope labeling reagent is [d0]/[D4]-acridone-10-ethyl-N-maleimide. The reagent adopts acridone as the isotope group and takes maleimide as the reaction group. The synthesis method includes: taking [d0]/[D5]-bromobenzene as the raw material, and carrying out two-step condensation reaction, hydroxylation reaction, helogenation reaction, Gabriel reaction and acylation reaction, thus obtaining the reagent. The stable isotope deuterium label obtained by the invention is subjected to separation and purification, then the isotope labeling position is table, the chemical purity is up to 99.0% or more, and the isotope abundance is up to 99.0% or more. The reagent can selectively label mercapto compounds, and has the advantages of fast labeling reaction rate, high labeling yield and high mercapto selectivity, etc.

The invention provides a non-diagnostic method for quantifying cfDNA by utilizing an ALU repetitive sequence and a ddcfDNA relative content detection method. The obtained data can be used for detecting organ transplantation rejection response.

The invention discloses a determination method for freshness of fresh cordyceps sinensis. The method comprises the following steps: cutting fresh cordyceps sinensis taken out from a storage environment into slices of which the thicknesses are 1 to 2 mm after carrying out pretreatment, then dyeing for a certain time by avoiding light at constant temperature in a TTC solution of which the concentration is 0.3%, rinsing, then cutting the dyed slices into fragments, carrying out extraction with ethanol at the constant temperature of 50 to 60 DEG C until cells become colorless, and collecting extract liquor and measuring the absorbance OD value at 485 nm. According to the invention, the activity of the fresh cordyceps sinensis is quantitatively detected through dehydrogenase produced by the respiration action of in-vivo cells of the fresh cordyceps sinensis, and the method is simple and easy to operate, stable and reliable in results and suitable for large-scale popularization and application.

The invention relates to the technical field of electrochemical sensing detection, in particular to an aminated multi-walled carbon nanotube electrochemical sensor and application thereof in quercetindetection. The invention discloses the aminated multi-walled carbon nanotube electrochemical sensor and application thereof in quercetin detection. The application comprises the following steps of: preparation of the aminated multi-walled carbon nanotube electrochemical sensor; and use of the electrochemical sensor for detecting quercetin. The electrochemical sensor overcomes the defects of too tedious method, complex steps and the like during quercetin detection in the prior art, better improves the detection sensitivity, is easy to achieve automation of quercetin detection.

The invention discloses a preparation method of integrase, which comprises the following steps: pyrolyzing a zinc-based zeolite imidazate framework material coated with mesoporous silicon, taking a generated porous metal nitrogen carbon material as a substrate, and loading gold nanoparticles in situ by utilizing a chemical reduction method to construct nano-enzyme; and finally, coupling the natural glucose oxidase with the nano-enzyme through electrostatic adsorption to obtain the integrase. According to the method, the catalytic activity of the nano enzyme can be reserved, and the stability and the catalytic activity of the natural enzyme can be improved. Besides, the prepared integrase can be used for further detecting glucose, the operation process is simple and rapid, the result is sensitive, the selectivity is high, the detection limit is low (0.77 mu M), the detection range is wide (1-300 mu M), and the integrase has a good application prospect in sensitive blood glucose detection. In addition, the integrase also has a three-in-one tumor treatment effect of hunger, chemical kinetics and photo-thermal.

The invention discloses a silver staining reagent and a staining method thereof. The invention provides a reagent for protein silver staining. The reagent provided by the invention comprises a staining liquid and a developing liquid; the solute of the straining liquid in the reagent comprises the following substances of ethanol, nitric acid and silver nitrate, wherein the proportion of the ethanol to the nitric acid to the silver nitrate is 25 ml: 1 ml: 0.2 g; and the developing liquid in the reagent comprises the following substances of sodium carbonate and formaldehyde, wherein the proportion of the sodium carbonate to the formaldehyde is 3 g: 0.2 ml. Proved by experiments, a novel silver staining method and a specific silver staining reagent are adopted in the invention, so that the operation is simple, the sensitivity is largely improved, and rapid, high-efficient and accurate detection of protein is realized after polyacrylamide gel electrophoresis.

The invention provides a visual nucleic acid detection method for simultaneously detecting one or a plurality of kinds of target nucleic acids. The method comprises the following steps of 1) preparing a reaction mixture which comprises one or a plurality of kinds of template DNA, DNA polymerase, DNA nicking enzyme 1, DNA nicking enzyme 2 and one or a plurality of kinds of probes; 2) putting the reaction mixture obtained in the step 1) at a constant temperature, and performing a constant-temperature index amplification reaction; 3) preparing a colloidal gold detection buffer solution; and 4) mixing the reaction product in the step 2) with the buffer solution in the step 3), putting a colloidal gold test strip in the solution for detection, and interpreting the result according to a detection strip. Compared with the prior art, the method provided by the invention has the following advantages that the method provided by the invention uses the test strip for visual reading, the operation is simple, the requirements on professional skills is low, special technical training is not needed, the application range is greatly expanded, and the detection cost is greatly reduced.