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Application of hemo as a marker of aging

A biomarker and aging technology, applied in the field of biomedicine, can solve problems such as the lack of in-depth research on specific connections, and achieve the effects of fast detection methods, low cost, and strong anti-interference ability.

Active Publication Date: 2022-05-03
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although ERVs have been found to be associated with aging, the specific link between the two has not been studied in depth. These studies have shown that ERVs are closely related to aging and related diseases

Method used

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  • Application of hemo as a marker of aging
  • Application of hemo as a marker of aging
  • Application of hemo as a marker of aging

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: RNA sequencing analysis of ERVs in senescent cells and verification of HEMO expression levels.

[0039] Based on the results of RNA sequencing and bioinformatics analysis, the present invention finds that ERVs are expressed differently between aging cells and young cells, and the endogenous retrovirus HEMO is found to be significantly up-regulated in aging cells. Using the senescence model of human fibroblasts and vascular endothelial cells, the corresponding young cells and senescent cells were prepared, and it was verified by fluorescent quantitative PCR (qPCR) that HEMO was indeed significantly up-regulated in senescent cells. To further verify the role of HEMO, we constructed lentiviral vectors expressing related ERVs, established stable cell lines overexpressing HEMO, and confirmed that the expression of HEMO induced human fibroblasts and Vascular endothelial cells. In addition, immunofluorescence experiments and cell survival curve experiments of γH2A....

Embodiment 2

[0082] Example 2: HEMO is highly expressed in brain tissue and skin cells of the elderly.

[0083] In the human body, it was found that the expression of HEMO was different in tissues of different ages, which fully demonstrated that HEMO was involved in the aging process. In the GEO database, through bioinformatics analysis, the expression of ERVs in individuals of different age groups was analyzed, and it was found that the expression of HEMO in the brain tissue and skin fibroblasts of the elderly population was significantly higher than that of the younger population. During human aging, both epigenetics and genome stability are changing, and the decrease of DNA methylation level at ERVs sites may be the reason for its upregulation. For a long time, factors affecting aging have always attracted much attention, and people are keen to explore aging markers and related diseases. The geriatric disease Alzheimer's disease is currently defined based on the presence of toxic prote...

Embodiment 3

[0086] Example 3: Blood samples demonstrate that HEMO is associated with aging.

[0087] Prove that HEMO is associated with aging using a small amount of clinical blood samples. In my country, people under the age of 40 are classified as young people, and people over 60 years of age are classified as the elderly. According to the characteristics that HEMO protein can be secreted into human blood, blood of healthy people of different ages was randomly sampled to detect the expression of HEMO.

[0088] 6 young people of different age groups and 7 elderly people of different age groups were selected, and 300ul blood samples were taken respectively. Whole blood protein extraction kits were used to extract the whole protein of these blood samples. After the protein was quantified, the transferrin Transferrin As an internal reference, ERVMER34-1 is used as an antibody to HEMO, and the expression level of HEMO can be detected by Western blot experiments, and it is found that the amou...

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Abstract

The invention discloses the application of HEMO as an aging marker. The present invention proposes for the first time a novel senescence marker—human endogenous MER34 open reading frame HEMO. The marker detection sample only needs a trace amount of blood sample, the detection method is fast, convenient, low cost, sensitive result, strong anti-interference ability, and can be developed into an Elisa kit for accurate quantification of HEMO.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to the identification of a biomarker in blood, which can be used to predict the health and aging states of a large number of subjects. Background technique [0002] The World Health Organization defines a society with an aging population as: the proportion of the population aged 65 and over reaches 7%. In recent years, the degree of population aging in my country has continued to increase, and aging and aging-related diseases have become a major problem that plagues human health and social development. In 2020, there will be about 180 million elderly people aged 65 and above in China, accounting for about 13% of the total population. The elderly group is an important consumer of medical and health resources. According to statistics from the Ministry of Health, the prevalence of chronic diseases among the elderly over the age of 60 is 3.2 times that of the entire population, and th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/7042
Inventor 丛羽生王雅香毛剑
Owner HANGZHOU NORMAL UNIVERSITY
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