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Nanogold test kit for early diagnosis of trichinization

A technology of Trichinella spiralis and nano-gold rods, which can be used in measuring devices, instruments, scientific instruments, etc., can solve the problems that the early diagnosis of parasitic diseases has not been reported and has not been widely used, and can improve the reaction rate and growth rate, with obvious advantages , Improve the effect of biological function

Inactive Publication Date: 2018-01-19
DALI UNIV
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  • Abstract
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Problems solved by technology

The physical and chemical characteristics of AuNPs make it possible to be widely used in the field of medical testing. Nano-gold labeling technology has also achieved certain results in the research of pathogen detection, but it has not been widely used, let alone in the early diagnosis of parasitic diseases. see the report

Method used

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  • Nanogold test kit for early diagnosis of trichinization
  • Nanogold test kit for early diagnosis of trichinization
  • Nanogold test kit for early diagnosis of trichinization

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preparation example Construction

[0033] In one embodiment of the present invention, a method for preparing trichinella antigen-labeled gold nanorods is provided, comprising the following steps:

[0034] 1) Reducing HAuCl with a reducing agent 4 Preparation of spherical gold nanoparticle seeds from solution;

[0035] 2) adding the spherical gold nanoparticle seeds to the growth medium, allowing the spherical gold nanoparticle seeds to grow into rod-shaped gold nanorods in the rod micellar solution;

[0036] 3) Use Trichinella spiralis muscle larvae to excrete and secrete antigens to label nano gold rods to obtain Trichinella spiralis antigen-labeled nano gold rods.

[0037] Preferably, in another embodiment of the present invention, the reducing agent in step 1) of the preparation method of Trichinella spiralis antigen-labeled gold nanorods is NaBH 4 .

[0038] Preferably, in another embodiment of the present invention, the method for preparing the spherical gold nanoparticle seeds prepared in step 1) of th...

Embodiment 1

[0047] Example 1 Preparation of nano gold rods

[0048] 1. Reagent chloroauric acid (HAuCl 4 ) Sangon Bioengineering (Shanghai) Co., Ltd.

[0049] Cetyltrimethylammonium bromide (CTAB) Sangon Bioengineering (Shanghai) Co., Ltd.

[0050] Silver nitrate (AgNO 3 ) Sangon Bioengineering (Shanghai) Co., Ltd.

[0051] Sodium borohydride (NaBH 4 ) Sangon Bioengineering (Shanghai) Co., Ltd.

[0052] Ascorbic acid (AA) Sangon Bioengineering (Shanghai) Co., Ltd.

[0053] nitric acid (HNO 3 ) Tianjin Zhiyuan Chemical Reagent Co., Ltd.

[0054] Hydrochloric acid (HCl) Tianjin Zhiyuan Chemical Reagent Co., Ltd.

[0055] 2. Instrument

[0056] UV Vis Spectrophotometer HACH / Hach (DR6000)

[0057] Waterproof constant temperature incubator Shanghai Yinxi Instrument Co., Ltd. (GHP-9080)

[0058] Electronic Balance Mettler-Toledo International Trading (Shanghai) Co., Ltd. (JA2003)

[0059] Pipette Gun Sangon Bioengineering (Shanghai) Co., Ltd.

[0060] Two-person single-sided purifi...

Embodiment 2

[0098] The preparation of the nano-gold biological probe of embodiment 2 Trichinella spiralis antigen labeling

[0099] 1. Reagents and materials

[0100]

[0101] Trichinella spiralis muscle larvae were provided by the Department of Parasitology, School of Basic Medicine, Dali University; healthy Kunming mice (about 25 g) were provided by the Animal Experiment Center of Dali University; positive serum was collected after artificially infecting the mice.

[0102] Preparation of normal saline: weigh 9g of sodium chloride, add pure water to make up to 1000mL, shake until fully dissolved, and store at 28°C.

[0103] Configuration of chloral hydrate: Weigh 10g of chloral hydrate, add pure water to make up to 100mL, shake to fully dissolve, and store at 28°C (ready for immediate use).

[0104] Traut’s reagent (5mg / mL) configuration: Weigh 100mg of Traut’s reagent, add pure water to 20mL, shake until fully dissolved, divide into 5 bottles, and store at -20°C.

[0105] The configu...

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Abstract

The invention discloses a preparation method of a trichinella spiralis antigen labeled gold nanorod. The method comprises the following steps: preparing spherical gold nanoparticle seeds by reducing an HAuCl4 solution with a reducing agent; adding the spherical gold nanoparticle seeds into a growth medium, and enabling the spherical gold nanoparticle seeds to grow into a rod-shaped gold nanorod ina rod micelle solution; labeling the gold nanorod by using an antigen excreted by trichinella spiralis larvae, thereby obtaining the trichinella spiralis antigen labeled gold nanorod. The invention further provides a trichinella spiralis antigen labeled gold nanorod prepared by the method. The gold nanorod is synthesized by selecting a 'seed mediated growth method', and the length-width ratio hasobvious advantages compared with a spherical nanorod; a gold nanorod labeling technology can be applied to serological test of trichinella spiralis, the detection kit is high in detected antibody dilution, early in time and low in infection gradient compared with a mouse serum antibody ELISA (Enzyme-linked Immuno Sorbent Assay) detection kit, and a novel technology can be provided for detecting early trichinosis infection and low infection gradient.

Description

technical field [0001] The invention belongs to the field of biological detection, in particular to a nano-gold test kit for early diagnosis of trichinella infection. Background technique [0002] Nanotechnology is a multidisciplinary science and technology that uses single atoms and molecules to create substances. The interval between the microscopic world represented by atoms and molecules and macroscopic objects between 1-100nm is the main research area of ​​nanotechnology. Nanoparticles represented by noble metals have special and excellent physical and chemical properties, so that they have received great attention in the fields of biosensing, biomedicine, and modernization of traditional Chinese medicine, and have become a research hotspot for scholars. [0003] As a typical noble metal, gold (Au) has a special size dependence, which makes it show great advantages and development prospects in medical inspection, diagnosis and treatment. The main special properties of...

Claims

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Application Information

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IPC IPC(8): G01N33/543
Inventor 杨毅梅唐亮何苗曹颖李成功张成桂
Owner DALI UNIV
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