Determination method for freshness of fresh cordyceps sinensis

A technology for fresh Cordyceps sinensis and Cordyceps sinensis is applied in the field of detection of raw materials of traditional Chinese medicinal materials, which can solve the problems of poor reproducibility of the detection of Cordyceps sinensis freshness, inability to accurately reflect the freshness of Cordyceps sinensis, and high experimental cost, and achieve easy learning and operation, dyeing and extraction time. The effect of shortening and low experimental cost

Active Publication Date: 2017-05-31
YICHANG SHANCHENGSHUIDU CORDYCEPS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to the complicated operation and long time-consuming of the above technical documents, there are problems such as poor reproducibility, high experimental cost, and inability to accurately reflect the freshness of Cordyceps sinensis when directly applied to the detection of the freshness of Cordyceps sinensis.

Method used

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  • Determination method for freshness of fresh cordyceps sinensis
  • Determination method for freshness of fresh cordyceps sinensis
  • Determination method for freshness of fresh cordyceps sinensis

Examples

Experimental program
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Effect test

Embodiment 1

[0040] To investigate the effects of different pretreatments on the freshness of fresh Cordyceps sinensis, the specific steps are as follows:

[0041] (1) Preparation of the test solution:

[0042] a. The same batch of 6 fresh Cordyceps sinensis stored at -5°C to -10°C for about 2 months was taken out from the storage environment and divided into two groups (5% glucose treatment group and room temperature treatment group), each group 3 roots. The 5% glucose treatment group was placed in a 5% glucose solution preheated to 37°C and treated in a 37°C water bath for 15 minutes, and the room temperature treatment group was placed at room temperature for 15 minutes, and then the body parts of 6 fresh Cordyceps sinensis were placed on the 4th- 7. Uniformly slice the foot section, with a thickness of 1-2mm;

[0043] b. Take 0.2g of 6 fresh Cordyceps slices in step a respectively, and every 3 same treatments are 1 group, totally 2 groups, and place them in 0.3% TTC solution (with pH ...

Embodiment 2

[0063] To investigate the influence of different parts of fresh Cordyceps sinensis on the determination of freshness, the specific steps are as follows:

[0064] (1) Preparation of the test solution:

[0065] a. Take the same batch of 3 fresh Cordyceps sinensis stored at -5°C to -10°C for about 2 months from the storage environment, place them in a 5% glucose solution preheated to 37°C and treat them in a 37°C water bath for 15 minutes, then Divide 3 fresh Cordyceps sinensis into 4 sections (fresh Cordyceps sinensis section, worm body head to 4th pair of feet section, worm body head 4-7 pair of foot sections, worm body head The seventh pair of feet to the tail section) is uniformly sliced, with a thickness of 1-2mm;

[0066] b. Take 0.2g of 4 different section sections of 3 fresh Cordyceps sinensis in step a respectively, and every 3 same sections form a group, totally 4 groups, and place them respectively in a 0.3% TTC solution with a mass fraction of 0.3% (pH 7.3-7.5 phosp...

Embodiment 3

[0079] The impact of different dyeing times on the freshness of fresh Cordyceps sinensis, the specific steps are as follows:

[0080] (1) Preparation of the test solution:

[0081] a. The same batch of 15 fresh Cordyceps sinensis stored at -5°C to -10°C for about 1 week was taken out from the storage environment, placed in a 5% glucose solution preheated to 37°C for 15 minutes in a 37°C water bath, and then 15 pieces of fresh Cordyceps sinensis worms from the head to the 4th to 7th pair of legs are sliced ​​evenly, with a thickness of 1-2mm;

[0082] b. Take 0.2g of 15 fresh Cordyceps slices in step a respectively, and every 3 pieces are 1 group, totally 5 groups, and place them respectively in 0.3% TTC solution (dissolved with phosphate buffer solution of pH 7.3-7.5) prepared) in 15 beakers at 37°C in a constant temperature water bath and protected from light for 0.5h, 1h, 2h, 4h, and 8h, respectively. After the staining time was 0.5h, 1h, 2h, 4h, and 8h, the fresh Cordyceps...

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Abstract

The invention discloses a determination method for freshness of fresh cordyceps sinensis. The method comprises the following steps: cutting fresh cordyceps sinensis taken out from a storage environment into slices of which the thicknesses are 1 to 2 mm after carrying out pretreatment, then dyeing for a certain time by avoiding light at constant temperature in a TTC solution of which the concentration is 0.3%, rinsing, then cutting the dyed slices into fragments, carrying out extraction with ethanol at the constant temperature of 50 to 60 DEG C until cells become colorless, and collecting extract liquor and measuring the absorbance OD value at 485 nm. According to the invention, the activity of the fresh cordyceps sinensis is quantitatively detected through dehydrogenase produced by the respiration action of in-vivo cells of the fresh cordyceps sinensis, and the method is simple and easy to operate, stable and reliable in results and suitable for large-scale popularization and application.

Description

technical field [0001] The invention relates to a method for detecting raw materials of Chinese medicinal materials, in particular to a method for measuring the freshness of fresh Cordyceps sinensis. [0002] technical background [0003] Cordyceps sinensis is a high-grade nourishing and precious Chinese herbal medicine. Traditionally, it has the effects of nourishing the lungs and kidneys, reducing phlegm and relieving cough. Modern research shows that Cordyceps sinensis has many other effects, and it is often used in the adjuvant treatment of malignant tumors, lowering blood pressure, lowering blood fat, regulating immune function, etc. The newly excavated fresh Cordyceps retains the nutrients to the greatest extent, and has a plump appearance and good taste, so fresh Cordyceps products have been born in recent years. During the process of unearthed, cleaned, sterilized, packaged and stored, fresh Cordyceps sinensis will cause certain damage to its freshness. As the fresh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N1/28
CPCG01N1/28G01N21/31
Inventor 艾中徐香琴钱正明李文佳李文庆
Owner YICHANG SHANCHENGSHUIDU CORDYCEPS
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