A kind of screening method of spotgrass breeding material

Abstract

The invention provides a screening method of a saccharum arundinaceum breeding material. The method comprises the following steps: collecting saccharum arundinaceum resources, and performing variation analysis of phenotypic character and correlation analysis among characters on the resources; extracting the genome DNA of saccharum arundinaceum as a template, adopting SCoT labeled primers to perform amplification, detecting amplified products through polyacrylamide electrophoresis, and obtaining a DNA finger-print chromatogram; performing polymorphic analysis and J genetic similarity coefficient analysis on the saccharum arundinaceum resources, and constructing UPGMA phylogenetic trees; performing comprehensive analysis on different ecological positions, phenotypic variation characteristics and phylogenetic trees of different saccharum arundinaceum resources, so as to obtain the screened material. By adopting the technical scheme and in combination with the phenotypic character variation analysis and a molecular marker technology, screening results are more sensitive and accurate; the utilization potential of saccharum arundinaceum germplasm resources is comprehensively evaluated, the accharum arundinaceum breeding material is screened, and the method can serve cane breeding.

Description

technical field [0001] The invention belongs to the technical field of agricultural breeding, and in particular relates to a method for screening breeding materials of spotted grass. Background technique [0002] Sugarcane is the most important sugar crop in my country and the most important economic crop in Guangxi. Modern sugarcane parents mainly have three bloodlines: tropical species, thin-stem wild species (cut hands dense) and Indian species. During the decades of "noble" breeding process, the genetic basis of sugarcane has gradually narrowed. Therefore, increasing the use of wild sugarcane resources to broaden the genetic basis of sugarcane is considered to be one of the key ways to achieve breakthroughs in sugarcane breeding. [0003] Banmao is an important wild germplasm of sugarcane. It has the advantages of drought resistance, barren tolerance, waterlogging tolerance, disease resistance, and insect resistance. It is currently a focus of research by sugarcane rese...

AI Technical Summary

Problems solved by technology

[0004] Banmao has rich phenotypic traits, and the phenotypic variation is used to analyze the utilization potential of Banmao, which is easily affected by the environment and ultimately affects the utilization potential of Banmao.

However, the current evaluation of the utilization of the germplasm resources of P. chinensis often only conducts the investigation and analysis of phenotypic traits, without combining the results of phenotypic variation with genetic analysis at the molecular level, and it is also impossible to obtain the results of genotype differences. The material and other information of Banmao

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