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Serum metabolic biomarker for acute anaphylaxis guinea pig

A biomarker, allergic reaction technology, applied in analytical chemistry and biochemistry, which can solve problems such as low sensitivity

Inactive Publication Date: 2018-04-13
湖南省药品检验研究院
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of traditional acute allergic reaction detection methods such as strong subjectivity and low sensitivity, and propose a set of detection methods for acute allergic reaction guinea pigs based on the ultra-high performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry platform. Serum metabolic biomarkers, which are objective, sensitive and reliable

Method used

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Embodiment Construction

[0029] (1) Clean guinea pigs (250-350g) were randomly divided into blank control group and acute allergic reaction group. In the acute allergic reaction group, bovine serum albumin (BSA) was used as a positive sensitizer, and the animals were given 10 mg / mL bovine serum albumin, 1.0 mL / head, intraperitoneal injection, once every other day, three consecutive times; 14th after the first sensitization On day (first attack) and on day 21 (second attack), the toe was injected intravenously with 50 mg / mL (2.0 ml / mouse) for challenge. The blank group was given the same volume of normal saline;

[0030] (2) Blood was collected from the orbit of the guinea pig before sensitization, after the third sensitization, and 30 minutes before and after the first and second attacks, and allowed to stand at room temperature for 10 minutes, centrifuged at 4°C, 3000rpm for 10 minutes, and the upper serum sample was taken at -80°C Keep in the refrigerator for later use;

[0031] (3) Mix the serum sampl...

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Abstract

The invention discloses a serum metabolic biomarker for an acute anaphylaxis guinea pig. The biomarker is lysophosphatidylcholine matter. The serums of sensitized guinea pig and healthy guinea pig aremixed with methanol to prepare to be sample solution; a technical platform for an ultra-high efficient liquid chromatogram-four-grade rod flight time tandem mass spectrum (UPLC-QTof / MS) is applied tocollect the serum metabolic spectrum of the guinea pig, and data is imported to EZinfo software to perform model identification and analysis. By comparing the serum of the of sensitized guinea pig with that of the healthy guinea pig, the lysophosphatidylcholine type (lysoPC (16: 0), lysoPC (18: 2), lysoPC (18: 0) metabolic substance level peforamnce is obviously improved; the abnormal change of the matter can be used for judging if the guinea pig is allergic or normal.

Description

Technical field [0001] The invention belongs to the technical field of analytical chemistry and biochemistry, and specifically relates to a serum metabolic biomarker for acute allergic reaction guinea pigs. Background technique [0002] Allergic reactions belong to the category of B-type adverse reactions. It is a hypersensitivity reaction that is completely unrelated to normal pharmacological effects and is immune-regulated. It is difficult to predict and has a low incidence, but a high mortality rate, accounting for about 5% to 10 of the total adverse drug reactions. %. Generally speaking, allergic reactions mostly refer to immediate type allergic reactions / type Ⅰ allergic reactions, which are strong body reactions that occur when the immune body is stimulated by the same allergen again, usually by immunogenic macromolecular substances or It is caused by the hapten small molecule substance combined with the macromolecular carrier. Allergens can stimulate the body’s lymphocyte...

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Application Information

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IPC IPC(8): G01N30/02G01N30/72G01N30/86G01N33/535G01N33/96
CPCG01N30/02G01N30/72G01N30/8634G01N30/8686G01N33/535G01N33/96G01N2570/00
Inventor 王蓉蓉李子珊陈子渊林海王湘波欧阳杰湖刘晓娟闫美玲陈萍
Owner 湖南省药品检验研究院
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