A DNA fingerprint of Hebawang and its acquisition method and special primers
A DNA fingerprint and map technology, applied in the biological field, can solve the problems of fewer bands and more primers, and achieve the effects of high polymorphism, good repeatability, and easy identification
Inactive Publication Date: 2018-06-26
SUGARCANE RES INST GUANGXI ACADEMY OF AGRI SCI +1
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Problems solved by technology
The methods used in the prior art to construct the DNA fingerprints of crop genomes are mainly molecular markers combined with polypropylene gel electrophoresis detection, which has the problem that the number of bands is relatively small and more primers are needed. In order to effectively use the Hebawang Abundant resources require the establishment of Hebawang's AFLP molecular marker technology system
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[0044] The preferred embodiments of the present invention will be further described in detail below in conjunction with the accompanying drawings.
[0045] Materials: 15 Hebawang clones were collected from different ecological regions in Guangxi, as shown in Table 1. At the same time, the altitude, longitude and latitude of the collection points were measured by GPS equipment, and the test materials were stored in the germplasm resource nursery of the Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences.
[0046] Table 1 The names and sources of 15 Hebawang germplasm resources
[0047] material code
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The invention provides DNA fingerprints of narenga, and an acquiring method and a special primer thereof. The special primer for acquiring the DNA fingerprints of narenga comprises a primer pair SEQ NO:1 and SEQ NO:2, a primer pair SEQ NO:3 and SEQ NO:4, and a primer pair SEQ NO:5 and SEQ NO:6. The DNA fingerprints of narenga acquired by the special primer provided by the invention are high in polymorphism and good in specificity and repeatability; the band of the fingerprints is within 100bp-400bp, the distribution is concentrated and uniform, the specificity is high, and the identification is convenient; the established genome DNA fingerprints of narenga have uniqueness, so that different genetic resources of narenga can be effectively and accurately identified, and the DNA fingerprints play an important role in genetic improvement of sugarcane by utilizing germplasm of narenga.
Description
technical field [0001] The invention belongs to the field of biotechnology, in particular to a DNA fingerprint of Hebawang, its acquisition method and special primers. Background technique [0002] Sugarcane is the most important sugar crop in my country, and its sucrose production accounts for more than 90% of the country's total sugar production. During the decades of "noble" breeding process, the genetic basis of sugarcane has gradually narrowed. Therefore, increasing the use of wild sugarcane resources to broaden the genetic basis of sugarcane is considered to be one of the key ways to achieve breakthroughs in sugarcane breeding. Narenga Bor [Narengaporphyrocoma (Hance) Bor] is a wild species of the genus Narenga Bor, also known as Caoxiomi, which has excellent traits such as thin tolerance, drought tolerance, coarse growth, early maturity, and strong tillering ability. Traditional breeding selection is to select for the variation of phenotypic traits, but some importan...
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IPC IPC(8): C12N15/11C12N15/10C12Q1/6895
Inventor 刘昔辉李杨瑞区惠平张荣华周会张革民杨丽涛宋焕忠杨柳李长宁张保青宋修鹏
Owner SUGARCANE RES INST GUANGXI ACADEMY OF AGRI SCI
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