Preparation method of Saccharum arundinaceum root-tip meristematic zone chromosome specimen

Abstract

The invention provides a preparation method of a Saccharum arundinaceum root-tip meristematic zone chromosome specimen, comprising: root-tip culture, root-tip sampling and pretreatment, root-tip enzymolysis, chromosome preparation, microscopic examination and other steps; the preparation method is characterized in that a whole-plant culture root system is planted in a bucket, the root tip is treated with 0.002 M of 8-hydroxyquinoline solution at 25-28 DEG C for 3-4 h, and the specimen is prepared by an enzymolysis wall degradation hypotonic method. The preparation method is highly operable, allows high-active meristematic zone root tip to be easily obtained, takes shorter time for root-tip culture, is low in manpower and material resource consumption and high in preparation efficiency; many interphases of meristematic zone cells are obtained; the obtained chromosome has vivid form and good dispersion uniformity, and is suitable particularly for the preparation of Saccharum arundinaceum chromosome specimens; the preparation method is also applicable to the preparation of chromosome specimens of sugarcane and plants in its related genera.

Description

technical field [0001] The invention relates to a method for preparing a plant chromosome metaphase specimen in plant cytogenetics, in particular to a method for preparing a root tip chromosome of a pagoda plant. [0002] technical background [0003] Chromosomes are carriers of genetic material, and chromosomes have a relatively stable number and shape in different species. Cytochromosomal genetic research plays an important role in genetic breeding, and the preparation of chromosome specimens is the basic experimental method for cytogenetic research. The preparation of plant chromosome specimens includes the following processes: root tip culture, material collection, pretreatment, fixation, enzymatic hydrolysis (or dissociation), slide preparation and microscopic examination. [0004] Spotted grass Erianthus arundinaceus (Retz.) Jeswiet. is a perennial herb, erect and clustered, without underground stems, widely distributed and highly adaptable. It has the characteristic...

AI Technical Summary

Problems solved by technology

However, most of the stems of P. chinensis are hollow, with fewer root points on the nodes, and fewer root points that can grow roots after moisturizing. The traditional method needs a large amount of stems of S. chinensis. In addition, in the process of cultivating the stems, The requirements for temperature and humidity control are high, and the root tips tend to brown and stop growing after they grow out, so it is not easy to obtain root tips with strong activity

It is very difficult to obtain better chromosome preparation by the traditional method of making magnolia chinensis, and researchers are looking for an efficient method for preparing chinensis chromosomes, which can obtain root tips with good activity in a short period of time, and has more The split-phase cells do not need to repeatedly cut stems for cultivation and root extraction, saving manpower and material resources, and improving the efficiency of chromosome preparation