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Method for screening drought-resistant variant sugarcane plants

A technology for mutant plants and sugarcane, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as cumbersome methods, and achieve the effects of simple operation, good drought tolerance and shortened time.

Active Publication Date: 2016-06-29
GUANGXI SUBTROPICAL CROPS RES INST GUANGXI SUBTROPICAL AGRI PROD PROCESSING RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports on sugarcane drought-tolerant breeding methods. The invention patent with the publication number CN101503692A discloses a method for cultivating drought-tolerant sugarcane varieties using the DREB2B gene. This technical method is cumbersome and requires expensive instruments such as gene guns. and resistance marker gene screening and other complex processes
From the perspective of variety popularization and application and food safety, there are still many barriers to the cultivation of transgenic plants, such as the ecological risk caused by gene drift to the environment, the selection marker genes such as antibiotics may still remain in plants, and the impact of genetically modified foods on human physiology and whether Uncertain factors such as toxicity are under intense debate in the scientific community

Method used

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  • Method for screening drought-resistant variant sugarcane plants
  • Method for screening drought-resistant variant sugarcane plants
  • Method for screening drought-resistant variant sugarcane plants

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Embodiment 1

[0039] A method for screening drought-tolerant mutants of sugarcane, comprising the following steps:

[0040] (1) The callus culture of sugarcane: the sugarcane buds up to 10cm in length were removed and sterilized, and then inoculated on the induction medium to induce callus. Each bottle was connected with 4 discs, cultured in the dark at first, continuously cultured for 10 days, and then carried out Light culture for 10 h / day, continuous culture for 15 days, to obtain callus; the specific operation of the disinfection and sterilization is as follows: take the sugarcane stems that are disease-free and grow robustly, wash them with running water, peel off the peripheral leaves, and put them on the ultra-clean workbench. First, peel the leaves layer by layer with a scalpel, and disinfect the surface with 75% alcohol for each peeling, leaving 0.5cm new leaves, and cutting them into 1mm thick discs; the induction medium contains 2.0mg / L2 , MS medium of 4-D, 0.1mg / LNAA;

[0041] ...

Embodiment 2

[0050] A method for screening drought-tolerant mutants of sugarcane, comprising the following steps:

[0051] (1) Callus culture of sugarcane: take out the sugarcane buds up to 20cm, sterilize and inoculate them on the induction medium to induce callus, take 5 discs in each bottle, culture in dark first, continue to cultivate for 10 days, and then carry out Light culture for 10 h / day, continuous culture for 15 days, to obtain callus; the specific operation of the disinfection and sterilization is as follows: take the sugarcane stems that are disease-free and grow robustly, wash them with running water, peel off the peripheral leaves, and put them on the ultra-clean workbench. Above, peel the leaves layer by layer with a scalpel, and disinfect the surface with 75% alcohol for each peeling, leaving 2cm new leaves and cutting them into 2mm thick discs; the induction medium contains 3.0mg / L2, 4-D, 0.2mg / L NAA MS medium;

[0052] (2) Embryogenic callus culture of sugarcane: transf...

Embodiment 3

[0061] A method for screening drought-tolerant mutants of sugarcane, comprising the following steps:

[0062] (1) The callus culture of sugarcane: the sugarcane buds up to 15cm in length were removed and sterilized, and then inoculated on the induction medium to induce callus. Each bottle was connected with 5 discs, cultured in the dark at first, continuously cultured for 10 days, and then carried out Light culture for 10 h / day, continuous culture for 15 days, to obtain callus; the specific operation of the disinfection and sterilization is as follows: take the sugarcane stems that are disease-free and grow robustly, wash them with running water, peel off the peripheral leaves, and put them on the ultra-clean workbench. First, peel the leaves layer by layer with a scalpel, and disinfect the surface with 75% alcohol for each peeling, leaving 1.5cm new leaves and cutting them into 1.5mm thick discs; the induction medium contains 2.5mg / MS medium of L2, 4-D, 0.15mg / LNAA;

[0063...

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Abstract

The invention discloses a method for screening drought-resistant variant sugarcane plants, belongs to the technical field of molecular breeding of sugarcane, and particularly relates to a selection breeding method for the drought-resistant sugarcane plants. The selection breeding method for the drought-resistant sugarcane plants comprises the steps that co-culturing is performed on small embryogenic suspension sugarcane cells through the appropriate saccharum arundinaceum total DNA solution concentration, cultures are cultured on MS culture media with the different PEG concentrations, repeated drought-resistant screening is performed on proliferation, differentiation and the like of calluses, SRAP molecular detection is performed on the variant plants, and then the drought-resistant sugarcane plants are finally selected and bred through drought-resistant stress selection in a greenhouse. According to the method, the sugarcane selection breeding time is shortened, operation is easy, and the cost is low; meanwhile, a novel path capable of breaking through close hybridization of sugarcane genera and species is supplied, and the obtained sugarcane plants have the better drought resistance than a control group.

Description

technical field [0001] The invention belongs to the technical field of sugarcane molecular breeding, and specifically relates to a method for breeding drought-tolerant sugarcane plants. Background technique [0002] Drought is a global problem. More than 50 countries and regions in the world belong to arid and semi-arid areas, accounting for 35% of the earth's total land area, while only 15% or less of the arable land is irrigated. China's dry land area is 78 million hm 2 , accounting for 60% of the total arable land area, of which the dry farming area without irrigation facilities accounts for 49.1% of the total arable land area. As the global climate shows a warming trend, drought will face more severe challenges. [0003] Sugarcane is an important sugar and energy crop in my country. Sugar is the main agricultural product related to the national economy and people's livelihood. China's main sugarcane producing areas are mainly distributed in Guangxi, Yunnan, Hainan and G...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 吕平檀小辉张继韦丽君黄强黄秋伟庞新华金刚黄显雅
Owner GUANGXI SUBTROPICAL CROPS RES INST GUANGXI SUBTROPICAL AGRI PROD PROCESSING RES INST
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