PCR primer pair used for identifying genetic sex of Pseudobagrus ussuriensis, and rapid identification method thereof

An identification method, Ussuri's technology, applied in DNA/RNA fragments, recombinant DNA technology, etc., can solve problems such as inability to apply, genetic background genetic material composition sex determination mechanism is different, etc. Effects of Economic and Social Values

Inactive Publication Date: 2016-10-12
HUAIYIN TEACHERS COLLEGE
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  • Abstract
  • Description
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Problems solved by technology

Whether it is hormone-induced sex reversal or mating after sex reversal, there is a problem: how to distinguish fish with the same biological sex but different genetic sex in a cultured group? How to identify normal male fish and super male fish, normal female fish and super female fish?
Different fish have different genetic backgrounds, genetic material compositions, and sex-determining mechanisms, so markers from one fish cannot be applied to another

Method used

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  • PCR primer pair used for identifying genetic sex of Pseudobagrus ussuriensis, and rapid identification method thereof
  • PCR primer pair used for identifying genetic sex of Pseudobagrus ussuriensis, and rapid identification method thereof

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Embodiment Construction

[0025] Attached below figure 1 , attached figure 2 Describe in detail the method for quickly identifying the genetic sex of the Ussuri catfish of the present invention, specifically implement according to the following steps:

[0026] (1) Individual physiological gender identification and serial number marking of the sample fish to be tested: first observe the appearance of the broodstock that needs to be gendered for good growth, determine the physiological sex according to the presence or absence of genital process and the position of the genital opening, record the number and hang a sign Card. Cut off the tail fin of the individual to be tested according to the corresponding number, the size of the thumbnail, and quickly put it into a centrifuge tube with a cover filled with 95% or absolute ethanol, and mark the number of the corresponding sample fish.

[0027] (2) Genomic DNA extraction: The genomic DNA extraction of the individual to be tested is carried out according ...

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Abstract

The invention discloses a PCR primer pair used for identifying the genetic sex of Pseudobagrus ussuriensis, and a rapid identification method thereof. The identification method comprises the steps of examined individual tissue drawing, genome DNA extraction, sex specific DNA fragment PCR amplification, agarose gel electrophoresis, ultraviolet light detection shooting and examined individual genetic sex determination. High-quality genome DNA is extracted through an optimized process, a sex specific DNA fragment is amplified, and the existence or not and the existence characteristic of the sex specific fragment are detected under ultraviolet lights, so the genetic constitution of Pseudobagrus ussuriensis is identified, and the genetic sex is discriminated in 1-2d. The PCR primer pair and the rapid identification method provide a rapid identification method for identifying sex reversal fish and super-male fish, provide technical support for breeding all-male fish, and have the advantages of breeding time shortening, great increase of the output, breeding cost reduction, and profound economic and social values.

Description

technical field [0001] The invention relates to the technical field of fish genetics and breeding, in particular to a pair of PCR primers and a rapid identification method for genetic sex identification of Ussuri catfish. Background technique [0002] Because many fishes have male and female dimorphism in terms of shape, growth rate, and sexually mature body weight, sex control and parthenogenesis are common and common practices in the aquatic industry. Parthenoculture can not only obtain commercial fish with neat specifications, improve the utilization rate of feed, increase the income of farmers and enterprises, but also promote the industrialization of modern fishery and form new varieties with independent intellectual property rights, which has broad market value and prospect. [0003] The sex of fish gonads is not only controlled by genetic factors, but also affected by the internal and external environment, especially the effect of exogenous sex hormones, which makes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6879
Inventor 潘正军朱传坤王辉强晓刚余祥胜常国亮丁怀宇吴楠
Owner HUAIYIN TEACHERS COLLEGE
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