SSR molecular marker and method for identifying authenticity of filial generation of sugarcane and saccharum arundinaceum

A molecular marker and sugarcane technology, applied in the field of genetic breeding, can solve the problems of insufficient marker genome coverage, chromosome loss, and inability to effectively detect hybrid offspring, etc., to achieve clear amplification bands, speed up the breeding process, and fast, accurate and authentic Effect

Active Publication Date: 2020-11-13
广东省科学院南繁种业研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the existing markers are developed based on individual materials, and the number of markers and the coverage of the genome are not enough. In the backcrossing and hybridization of sugarcane and scorpion grass, due to the loss of chromosomes, the real hybrid offspring cannot be effectively detected.

Method used

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  • SSR molecular marker and method for identifying authenticity of filial generation of sugarcane and saccharum arundinaceum
  • SSR molecular marker and method for identifying authenticity of filial generation of sugarcane and saccharum arundinaceum
  • SSR molecular marker and method for identifying authenticity of filial generation of sugarcane and saccharum arundinaceum

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Embodiment 1

[0090] This embodiment has selected 5 different DNA mixtures of the original species of Banana (Jiangxi 83-4, Sichuan 79-I-9, Hainan 92-77, Guizhou 78-II-14), 3 sugarcane parent material DNA mixtures (tropical species Badila .

[0091] Step 1, first extract the genomic DNA of the material, and use it as a template for standby;

[0092] (1) Extraction of genomic DNA

[0093] Take the CTAB extraction method:

[0094] 1) Take the young leaves of the material, grind them with liquid nitrogen, add about 200mg of tissue samples into a 1.5mL centrifuge tube;

[0095] 2) Add 700 μL of lysis buffer preheated at 65°C, bathe in water at 65°C for 30 minutes, and turn over evenly 2-3 times during the period;

[0096] 3) Add 700 μL of chloroform / isoamyl alcohol (chloroform: isoamyl alcohol: 24:1) mixture, shake vigorously for 10 minutes, then centrifuge at 11000 rpm and 15°C for 10 minutes, and transfer the supernatant into a 1.5 mL centrifuge tube;

[0097] 4) Add an equal volume of pr...

Embodiment 2

[0114] Example 2 Identification of Pseudomonas ancestry in different backcross generations of sugarcane and Pseudomonas hybrids

[0115] The hybrid combination of 3 sugarcane cultivars (Xintaitang 22, Yuetang 00-236, Yuetang 91-976) and Banmao high-generation backcross materials (Yacheng 07-71, Yacheng 06-140) was selected:

[0116] Hybrid combination 1: Xintai Sugar No. 22 × Yacheng 07-71;

[0117] Hybrid combination 2: Yuetang 00-236×Yacheng 07-71;

[0118] Hybrid combination 3: Yuetang 91-976×Yacheng 06-140.

[0119] Seedlings were sown in the 3 hybrid combinations according to conventional methods, and sugarcane seedlings were sown in a greenhouse seedling box in sunny weather. Seedling-growing substrate, then sprinkled with sterilized sand; spray the seedling-raising substrate with water until the whole seedling-raising substrate is soaked, and finally cover with plastic film;

[0120] The seedling-raising substrate is prepared through the following steps: soil and bag...

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Abstract

The invention belongs to the field of genetic breeding. The invention particularly relates to an SSR molecular marker and method for identifying authenticity of filial generations of sugarcane and saccharum arundinaceum. The SaSSR molecular marker is amplified by adopting 10 primer pairs with nucleotide sequences as shown in SEQ ID NO: 1-20, an amplification product is detected by agarose gel electrophoresis, and the authenticity of the filial generation of sugarcane and saccharum arundinaceum is identified by analyzing strips. The SaSSR molecular marker disclosed by the invention is a group of specific SSR markers with different amplification bands between sugarcane and saccharum arundinaceum, uniform distribution of the markers in different chromosomes of saccharum arundinaceum is ensured, and the SSR molecular marker and method are particularly suitable for identification of sugarcane and saccharum arundinaceum high-generation materials.

Description

technical field [0001] The invention belongs to the field of genetic breeding, and in particular relates to an SSR molecular marker and a method for identifying the authenticity of hybrid offspring of sugarcane and scalloped grass. Background technique [0002] Sugarcane is one of the important sugar crops and energy crops. The development of sugarcane production plays an important role in promoting the development of agriculture and related industries. At present, the development of sugarcane mainly focuses on cultivating new varieties of sugarcane with high yield, high resistance and high sugar, and new varieties of sugarcane can be realized through conventional hybrid breeding or transgenic breeding. Carrying out distant hybridization and introducing excellent genes from wild resources into cultivated species is an important way to improve the adaptability, stress resistance and perennial root of sugarcane varieties. Banmao is one of the closely related species of sugarc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 齐永文黄郑晖崔方庆曾巧英
Owner 广东省科学院南繁种业研究所
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