A method for inducing plant regeneration from embryoid bodies of head cabbage

A technology for regenerating plants and cabbage, applied in the field of plant tissue culture, can solve the problems of low induction rate and low induction rate of microspores, and achieve the effects of high value-added coefficient and high genetic stability

Inactive Publication Date: 2016-08-17
重庆市黔江区阿蓬科技企业孵化器有限公司
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, using microspores as explants to directly induce regenerated plants or induce embryoid bodies or induce germination, the induction rate is not high, such as Chinese patent ZL201010018236. The embryo emergence rate of the combination is 10.9 embryos / bud, and the induction rate of microspores is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. Induction of sterile seedlings: select firm and plump cabbage seeds, clean the seeds with tap water, disinfect them in 70-75% ethanol for 15 seconds, and disinfect them in a sodium hypochlorite solution with a volume concentration of 3% for 10 minutes. Wash the seeds with tap water after autoclaving and cooling; inoculate the sterilized seeds in the sterile seedling induction medium, and cultivate them in a sterile culture room: the culture conditions are temperature 18-22°C, light 1500-2000Lx, air humidity 50%-60%, when the sterile seedlings are cultivated to 5-8cm, take the sterile seedlings out of the cultivation room, and clean them with cooled high-pressure sterilized water;

[0018] 2. Induction of embryoid bodies: In an aseptic operating table, select the hypocotyls of the sterile cabbage seedlings as explants, cut the hypocotyls into 3-4mm segments, and inoculate them in the embryoid body induction medium 5 explants were inoculated in each bottle of medium, a...

Embodiment 2

[0025] The induction process is the same as in Example 1, except that the embryoid body induction medium formula is: add 1mg / L indoleacetic acid, 0.1mg / L 6-glycosylaminopurine, 30g / L sucrose in every 1L MS medium , 7g / L agar; the medium formula for regenerated plants is: add 1mg / L naphthaleneacetic acid, 0.5mg / L 6-glycosylaminopurine, 30g / L sucrose, and 7g / L agar to each 1L MS medium. The induction rate of embryoid bodies was 85%, and the induction rate of regenerated plants was 88%.

Embodiment 3

[0027] The induction process is the same as in Example 1, except that the embryoid body induction medium formula is: add 1.5mg / L indoleacetic acid, 1mg / L 6-glycosylaminopurine, 30g / L sucrose in every 1L MS medium , 7g / L agar; the medium formula for regenerated plants is: add 1.5mg / L naphthaleneacetic acid, 1mg / L 6-glycosylaminopurine, 30g / L sucrose, and 7g / L agar to each 1L MS medium. The induction rate of embryoid bodies was 80%, and that of regenerated plants was 75%.

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PUM

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Abstract

The invention discloses a method for inducing embryoid bodies of cabbage, which includes induction of sterile seedlings, induction of embryoid bodies, induction of regenerated plants, seedling hardening and transplantation. directly induce regeneration plants or induce embryoid bodies or induce budding, and the induction rate is not high; the following hypocotyls of the present invention are used as explants for inducing embryoid bodies, and the induction rate can reach 90%, and the embryoid bodies induce regenerated plants. The induction rate can reach 90%; the regenerated plants are induced by embryoid bodies, and the regenerated plants have high genetic stability, no physiological isolation from the mother body, and a high value-added coefficient.

Description

technical field [0001] The invention relates to a method for cultivating a regenerated plant of cabbage embryoid body, belonging to the field of plant tissue culture. Background technique [0002] Heading Brussels Sprouts ( Brassica oleracea L.var.capitata L.) is a plant of Brassicaceae and Brassica genus, which is cabbage ( Brassica oleracea L.), also known as roll cabbage, cabbage and cabbage. Cabbage has cold resistance, disease resistance, strong adaptability, easy storage, high yield, and good quality. It is one of the main vegetables in northern my country. [0003] The traditional breeding method is to use fine varieties for self-segregation. This breeding method has a long cycle and a low probability of obtaining high-quality varieties. Since Lichter reported for the first time in 1982 that rape free microspores were used to obtain regenerated plants, Brassica crops have achieved great success in terms of embryo emergence rate, embryo quantity, embryo culture an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 苏建丽
Owner 重庆市黔江区阿蓬科技企业孵化器有限公司
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