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A method for tissue culture, rapid propagation and in vitro preservation of Baoxing lily

A technology of in vitro preservation and tissue culture rapid propagation, which is applied in the field of plant biology, can solve the problems of unsatisfactory multiplication and in vitro preservation and subculture cycle, so as to prolong the in vitro preservation period and facilitate preservation and development and utilization Effect

Active Publication Date: 2017-03-29
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Baoxing lily has been explored in tissue culture rapid propagation and in vitro preservation, it is still unsatisfactory in terms of proliferation and in vitro preservation subculture cycle. For example, Fu Yiqian (2012, Several wild lily in vitro preservation techniques research) using the leaves of Baoxing lily as explants, in MS+NAA2.0mg / L+TDZ 0.2mg / L medium, its value-added coefficient is only 3.29, in 1 / 2MS+NAA0.5mg / L+activated carbon In the in vitro preservation medium of 2g / L, the subculture cycle is only 1 month

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Material: Lilium duchartrei Franch.

[0023] 2. Material category: middle scales of Baoxing Lily.

[0024] 3. Culture conditions: (1) Adventitious bud induction and multiplication medium: MS+BA 0.5mg / L+2,4-D 1.0mg / L; (2) Bulb enlargement and rooting medium: MS+PPP 333 2.0mg / L+NAA 0.5mg / L; (3) In vitro preservation medium: MS+PPP 333 0.3mg / L+BA 0.5mg / L+NAA 0.5mg / L+TDZ0.06mg / L. The above medium (1) and (3) added sucrose 20g / L, (2) added sucrose 90g / L, (2) and (3) added activated carbon 1-2g / L. All mediums are solidified by adding 5.6g / L agar, pH 5.8. Except for in vitro storage and culture conditions of 13±2℃, the rest culture temperature is 25±2℃, and light intensity is 10-20μmol / (m 2 ·S), illumination 12h / d.

[0025] 4. Growth and differentiation

[0026] 4.1. Obtaining explants: Obtain the bulbs of Baoxing lily from the field, wrap them in wet moss and bring them back to the laboratory. Wash the soil on the surface of the bulbs with gauze. Select the middle scales and add ...

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Abstract

A lilium duchartrei franch tissue culture rapid propagation and separation preservation method comprises the steps of obtaining explant, inducing adventitious buds, carrying out enrichment culture, enlarging the bulb, carrying out rooting culture, carrying out separation preservation and carrying out transplanting. A scale of lilium duchartrei franch serves as the explant, a tissue culture rapid propagation and separation preservation system of the lilium duchartrei franch is established, the appreciation coefficient highly reaches 1:10, and the separation preservation subculture period reaches four years or longer. By means of the method, the problem of tissue culture rapid propagation of the lilium duchartrei franch is effectively solved, the separation preservation period is greatly prolonged, the preservation, the development and the utilization of the germplasm resources of the lilium duchartrei franch are facilitated, and a foundation is laid for breeding of the lilium duchartrei franch.

Description

[0001] Technical field: [0002] The present invention relates to the field of plant biotechnology, in particular to a method for tissue culture, rapid propagation and in vitro preservation of the plant Lilium duchartrei Franc. [0003] Background technique: [0004] Lilium duchartrei Franch. (Lilium duchartrei Franch.) is a lily plant in the lily family, endemic to China. It is mainly distributed in my country's Sichuan, Yunnan, Tibet and Gansu provinces. It is born in alpine grasslands, forest edges or shrubs at an altitude of 2300-3500 meters. in. Baoxing lily is the only wild species with fragrance, white flowers and recurved petals among Asian lilies. Baoxing Lily is used as medicine with bulbs to moisturize the lungs and relieve cough, clear away heat and soothe the nerves. It can be used to treat tuberculosis, chronic bronchitis and other diseases. Baoxing lily is a potential parent for cultivating fragrant flower lily varieties. It is not only an excellent germplasm material...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G1/00
Inventor 何俊孟静李春芳程治英杨俊波李德铢
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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