Rapid propagation method for apple tissue culture seedlings

A technology for tissue cultured seedlings and apples, applied in the field of rapid propagation of apple tissue cultured seedlings, can solve the problems of poor application effect, low transplanting survival rate, poor repeatability of apple tissue cultured seedlings rapid propagation technology, etc.

Pending Publication Date: 2020-11-10
昭通市苹果产业发展中心
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In summary, the existing problems in the prior art are: poor repeatability of apple tissue cult

Method used

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  • Rapid propagation method for apple tissue culture seedlings
  • Rapid propagation method for apple tissue culture seedlings
  • Rapid propagation method for apple tissue culture seedlings

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Experimental program
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Effect test

Embodiment

[0020] First prepare the subculture proliferation medium, rooting medium culture and transplanting matrix; wherein, the subculture proliferation medium is used for the subculture proliferation of apple tissue cultured seedlings, and the rooting medium is used for the rooting culture of tissue cultured seedlings , the transplanting substrate is used for transplanting when the rooting of tissue culture seedlings meets the requirements of transplanting.

[0021] Subculture medium:

[0022] Add about 13L of distilled water into the filling machine, add 40g of polyvinyl alcohol, and then add 120g of agar, 800g of sucrose, 38g of potassium nitrate, 33g of ammonium nitrate, 7.4g of magnesium sulfate heptahydrate, 3.4g of potassium dihydrogen phosphate, and Calcium chloride hydrate 8.8g, manganese sulfate tetrahydrate 0.446g, zinc sulfate heptahydrate 0.172g, boric acid 0.124g, potassium iodide 0.0166g, sodium molybdate dihydrate 0.005g, copper sulfate pentahydrate 0.0005g, cobalt chl...

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Abstract

The invention provides a rapid propagation method for apple tissue culture seedlings. The method comprises the following steps of culturing the apple tissue culture seedlings for 30-40 days by using asubculture multiplication culture medium; then transferring the apple tissue culture seedlings to a rooting culture medium and culturing the apple tissue culture seedlings for 25-35 days; and finallytransplanting the rooted tissue culture seedlings to a transplanting substrate by using a nutrition pot. By utilizing the multiplication culture medium provided by the method, the multiplication coefficient reaches 6.1 on average and is increased by 8.9 % compared with that obtained through the prior art; by utilizing the rooting culture medium provided by the method, the rooting effect is achieved, and the rooting rate of tissue culture seedlings is remarkably improved, which reaches 85 % on average, and is increased by 6.25 % compared with that obtained through the prior art; and by utilizing the transplanting substrate provided by the method, the survival rate of tissue culture seedlings is increased and reaches 75 % or above, the highest survival rate can reach 85 %, and the survivalrate is increased by 6.25 %-7.14 % compared with that obtained through the prior art.

Description

technical field [0001] The invention relates to the technical field of apple seedling breeding, in particular to a technology for rapid propagation of apple tissue culture seedlings. Background technique [0002] Apple seedling breeding techniques include cuttings, layering, grafting, tissue culture, etc. The rooting rate and survival rate of cutting apple branches are low, and it is difficult to obtain a large number of plants in a short time; the propagation coefficient of layering is low, the cost is high, and the technical requirements are difficult; grafting can easily lead to the rapid spread of apple virus diseases, thereby affecting the quality of seedlings and affecting The quality of garden construction and the cost of later management; tissue culture can quickly obtain a large number of plants in a short time, with low cost and high benefit, and breeding virus-free tissue culture seedlings can greatly increase the cost of seedlings. [0003] At present, the rapid...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张丹张秀英李超马勉娣李云国胡志芳王顺富汪琼杜楠谭安超姚梅陈晨刘毅黄先月杨渊
Owner 昭通市苹果产业发展中心
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