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Tissue culture rapid breeding method of Chinese medicine abrotanum

A technology of tissue culture and Artemisia annua, which is applied in the field of plant propagation, can solve the problems of restricting the promotion and use of artemisinin drugs and the high market price of artemisinin, and achieve the effects of stable content, easy material acquisition, and high value-added coefficient

Inactive Publication Date: 2011-03-30
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These problems lead to the high market price of artemisinin, which seriously limits the popularization and use of artemisinin drugs in poor and backward malaria-infested areas. Content

Method used

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  • Tissue culture rapid breeding method of Chinese medicine abrotanum
  • Tissue culture rapid breeding method of Chinese medicine abrotanum
  • Tissue culture rapid breeding method of Chinese medicine abrotanum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1, Tissue Culture Rapid Propagation of Chinese Medicine Artemisia annua

[0021] 1. Preparation and sterilization of culture medium

[0022] Primary medium: MS+6-BA 0.5mg / L+GA 1.0mg / L.

[0023] Proliferation medium: MS+6-BA 1.5mg / L+NAA 0.2mg / L.

[0024] Rooting medium: MS+IBA 0.5mg / L.

[0025] The above medium was sterilized at 121.3°C for 20 minutes.

[0026] 2. Primary culture

[0027] Take the terminal bud or axillary bud of the plant of the traditional Chinese medicine Artemisia annua L., soak it with 70% (volume percentage) alcohol for 1 minute, and then put it into 0.1% (mass percentage) mercury chloride solution for sterilization After 5-10 minutes, after rinsing with sterile water for 3 times, the terminal buds or axillary buds were transferred into a triangular flask (capacity 250ml) equipped with primary medium, and the number of explants in each triangular flask was 5. The culture was carried out under the following culture conditions: 25° C., li...

Embodiment 2

[0044] Example 2, Tissue Culture Rapid Propagation of Chinese Medicine Artemisia annua

[0045] 1. Preparation and sterilization of culture medium

[0046] Primary medium: MS+6-BA 0.1mg / L+GA 0.2mg / L.

[0047] Proliferation medium: MS+6-BA 0.5mg / L+NAA 0.1mg / L.

[0048] Rooting medium: MS+IBA 0.2mg / L.

[0049] The above medium was sterilized at 121.3°C for 20 minutes.

[0050] 2. Primary culture

[0051] Take the terminal bud or axillary bud of the traditional Chinese medicine Artemisia annua L., soak it with 70% (volume percentage) alcohol for 1 minute, and then put it into 0.1% (mass percentage) mercuric chloride solution to sterilize for 5 -10 minutes, after rinsing with sterile water for 3 times, the terminal buds or axillary buds were transferred to a triangular flask (capacity 250ml) equipped with the primary culture medium, and the number of explants in each triangular flask was 5. Cultured under the following culture conditions:

[0052] 22°C, 14 hours of light and...

Embodiment 3

[0063] Example 3, Tissue Culture Rapid Propagation of Chinese Medicine Artemisia annua

[0064] 1. Preparation and sterilization of culture medium

[0065] Primary medium: MS+6-BA 2.0mg / L+GA 2.0mg / L.

[0066] Proliferation medium: MS+6-BA 2.0mg / L+NAA 0.5mg / L.

[0067] Rooting medium: MS+IBA 1.0mg / L.

[0068] The above medium was sterilized at 121.3°C for 20 minutes.

[0069] 2. Primary culture

[0070] Take the terminal bud or axillary bud of the traditional Chinese medicine Artemisia annua L., soak it with 70% (volume percentage) alcohol for 1 minute, rinse it with sterile water for 3 times, and then add 0.1% (mass percentage) mercuric chloride solution for 5-10 minutes, after rinsing with sterile water for 3 times, transfer the terminal buds or axillary buds into a triangular flask (capacity 250ml) equipped with primary culture medium, the number of explants in each triangular flask for 5. Cultured under the following culture conditions:

[0071] 30°C, 16 hours of lig...

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Abstract

The invention discloses a fast propagation method of tissue culture of traditional Chinese medicine Artemisia annua. The fast propagation method of tissue culture of traditional Chinese medicine Artemisia annua is that culture a terminal bud or an axillary bud of Artemisia annua are cultured in an initial medium so as to obtain an aseptic seedling. The initial medium is a medium of MS medium added with 6-BA and GA. The final concentration of the 6-BA is 0.1 to 2.0 mg / l and the GA is 0.2 to 2.0mg / l. The invented method has the advantages of easy avail of materials and aseptic materials, low variation rate, high enhancement factor, 5 to 6 times of multiple in 4 weeks, over 93 percent of rooting rate of tissue culture seedlings, over 95 percent of survival rate of transplanting and very strong commercialized production capacity. The high productive Chinese medicine Artemisia annua is reproduced by making use of the invention, and the high arteannuin of the offspring can be stabilized, thus increasing the output of arteannuin.

Description

technical field [0001] The invention relates to a plant propagation method, in particular to a tissue culture rapid propagation method of the traditional Chinese medicine Artemisia annua L. Background technique [0002] Artemisinin, an effective antimalarial monomer isolated from the traditional Chinese medicine Artemisia annua L., is the first drug independently invented by Chinese scientists and recognized globally. The anti-malarial drugs developed based on artemisinin are highly valued by the World Health Organization (WHO). Malaria is the most widespread tropical parasitic infectious disease with the highest morbidity and mortality in the world today. According to the statistics report of the World Health Organization in recent years: there are currently 2.9 billion people living in malaria-endemic areas in the world, with an annual incidence of 300-500 million and a death toll of 2-3 million. Malaria has become the number one killer in Africa. In order to control the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 叶和春李国凤王红颜芳刘本叶
Owner INST OF BOTANY CHINESE ACAD OF SCI
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