Method for subculturing camphorwood tissue culture seedlings

Abstract

The invention provides a method for subculturing camphorwood tissue culture seedlings. The formulation of a subculture medium for the culture method comprises 1 / 2 MS of culture medium, 2.5-4 mg / L of 6-BA (6-Benzyl Aminopurine), 0.1-0.5 mg / L of NAA (Naphthyl Acetic Acid), 6.5 g / L agar and 30 g / L of sucrose. Multiple subcultures are carried out by using the same subculture formulation, the multiplication factor can be increased to achieve the purpose of propagation, industrial production can be carried out, and a great number of nursery stocks are provided for the market. The method for subculturing the camphorwood tissue culture seedlings, provided by the invention, is beneficial to not only simplification of the operating process, but also reduction of the production cost, thereby achieving higher economic benefits.

Description

technical field

[0001] The invention relates to a subculture method of camphor tree tissue culture seedlings. Background technique

[0002] Camphor wood has fine texture, flexibility and compactness, smooth and beautiful, contains volatile oil and special fragrance, moisture resistance, corrosion resistance, insect repelling, small deformation, long shelf life, and is a good material for valuable furniture, construction, cabinets, carvings, and shipbuilding. Camphor and camphor oil can be extracted from roots, branches, leaves and wood. Camphor and camphor oil are important raw materials in modern medicine, metallurgy, chemical industry, spices, food industry and military industry. They are precious materials and special economic tree species in southern my country. But the seedlings were severely differentiated. Asexual reproduction can maintain the excellent traits of the female parent. As a tissue culture and rapid propagation technology for seedlings, although there are ...

Examples

Embodiment Construction

[0010] 1. Medium screening

[0011] After the primary culture of the camphor plantlets, the axillary buds grow to 1.5-2.0 cm, the axillary buds are cut from the explants, and the axillary buds are transferred to the subculture medium.

[0012] The screening of medium formula for subculture experiment adopts L9 (3 4 ), as shown in Table 1, the cytokinin is 6-BA, the auxin is NAA, IBA. After 30 days of inoculation, count the number of effective shoots that can be subcultured as a method for evaluating the value-added coefficient.

[0013] Table 1 Orthogonal test design for the selection of subculture medium of Cinnamomum camphora

[0014]

[0015] After the subculture of camphor plantlets, the optimal subculture medium formula was screened by orthogonal design method: modified MS medium with halved macronutrients + 6-BA3.0 mg / L + NAA0 .4 mg / L + agar 6.5g / L + sucrose 30g / L, the value-added coefficient is as high as 6 or more. Such as figure 1 As shown, using the same subc...

technical field

[0001] The invention relates to a subculture method of camphor tree tissue culture seedlings. Background technique

[0002] Camphor wood has fine texture, flexibility and compactness, smooth and beautiful, contains volatile oil and special fragrance, moisture resistance, corrosion resistance, insect repelling, small deformation, long shelf life, and is a good material for valuable furniture, construction, cabinets, carvings, and shipbuilding. Camphor and camphor oil can be extracted from roots, branches, leaves and wood. Camphor and camphor oil are important raw materials in modern medicine, metallurgy, chemical industry, spices, food industry and military industry. They are precious materials and special economic tree species in southern my country. But the seedlings were severely differentiated. Asexual reproduction can maintain the excellent traits of the female parent. As a tissue culture and rapid propagation technology for seedlings, although there are ...

Examples

Embodiment Construction

[0010] 1. Medium screening

[0011] After the primary culture of the camphor plantlets, the axillary buds grow to 1.5-2.0 cm, the axillary buds are cut from the explants, and the axillary buds are transferred to the subculture medium.

[0012] The screening of medium formula for subculture experiment adopts L9 (3 4 ), as shown in Table 1, the cytokinin is 6-BA, the auxin is NAA, IBA. After 30 days of inoculation, count the number of effective shoots that can be subcultured as a method for evaluating the value-added coefficient.

[0013] Table 1 Orthogonal test design for the selection of subculture medium of Cinnamomum camphora

[0014]

[0015] After the subculture of camphor plantlets, the optimal subculture medium formula was screened by orthogonal design method: modified MS medium with halved macronutrients + 6-BA3.0 mg / L + NAA0 .4 mg / L + agar 6.5g / L + sucrose 30g / L, the value-added coefficient is as high as 6 or more. Such as figure 1 As shown, using the same subc...

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