Method for subculturing camphorwood tissue culture seedlings
A technology of subculture and subculture medium, applied in the field of subculture of camphor tissue culture seedlings, can solve the problems of low proliferation coefficient, reduced value-added coefficient, different formulas, etc., to simplify the operation process, improve the value-added coefficient, The effect of reducing production costs
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[0010] 1. Medium screening
[0011] After the primary culture of the camphor plantlets, the axillary buds grow to 1.5-2.0 cm, the axillary buds are cut from the explants, and the axillary buds are transferred to the subculture medium.
[0012] The screening of medium formula for subculture experiment adopts L9 (3 4 ), as shown in Table 1, the cytokinin is 6-BA, the auxin is NAA, IBA. After 30 days of inoculation, count the number of effective shoots that can be subcultured as a method for evaluating the value-added coefficient.
[0013] Table 1 Orthogonal test design for the selection of subculture medium of Cinnamomum camphora
[0014]
[0015] After the subculture of camphor plantlets, the optimal subculture medium formula was screened by orthogonal design method: modified MS medium with halved macronutrients + 6-BA3.0 mg / L + NAA0 .4 mg / L + agar 6.5g / L + sucrose 30g / L, the value-added coefficient is as high as 6 or more. Such as figure 1 As shown, using the same subc...
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