A method for inducing grape fruit callus and its culture medium

Abstract

The invention discloses an induction method for a grape fruit callus and a culture medium thereof. The culture medium comprises the composition: a basal culture medium, 0.8-1.2 mg / L of TDZ, 0.3-0.5 mg / L of IBA, 18-22 g / L of sugar, and 5-7 g / L of agar. The induction method is characterized by comprising the steps: sterilizing grape fruits, then peeling, taking pulp blocks, inoculating the inductionculture medium with the pulp blocks, and carrying out induction culture until the callus is formed. The culture method of the fruit callus of 'Xiahei' grapes is successfully established, and the induction rate of the fruit callus reaches 100%, a new way is provided for molecular biology and genetic engineering research of the 'Xiahei' grapes, a new experimental material is provided for the genetic engineering research and gene transformation of the grapes, and meanwhile, a reference is also provided for culture of the grape fruit callus.

Description

Technical field [0001] The invention relates to the field of plant biotechnology, in particular to a method for culturing grape fruit pulp callus. Background technique [0002] At present, the research of grape genome is a hot spot in grape science. The research direction of grape genome mainly involves plant resistance, fruit quality and fruit production characteristics. Among them, the important economic characteristics of grapes are especially the molecules related to the formation of fruit quality. Mechanism research has also become one of the key issues in grape genome research. The ultimate goal of molecular mechanism research is to verify gene function. However, because the transgenic technology of grapes is not mature enough, the efficiency of gene transformation is extremely low, and the transgenic cycle is long and time-consuming. Callus is a good material for verifying gene function. Callus refers to a group of non-polar and vigorously dividing parenchymal cells forme...

AI Technical Summary

Problems solved by technology

Only studying the callus of grape leaves cannot meet the requirements of scientific research, especially for grape fruit, the fruit itself must be studied in order to achieve the purpose of producing high-quality fruit

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