Improve the method of tissue culture multiplication and strong seedling of bamboo root ginger

Abstract

The invention discloses a method for improving tissue culture, proliferation and seedling expansion effects of a bamboo-root ginger. The method comprises the following steps: cluster bud induction, cluster bud subculture multiplication, tube seedling cultivation, tube seedling propagation and the like, wherein in the cluster bud induction, the subculture medium is obtained by the following steps: by taking MS as a basic culture medium, inoculating 1.5mg / L of BA into the basic culture medium, and transferring to the basic culture medium containing 1.5mg / L of BA and 0.2mg / L of NAA after 20d, wherein the concentration of NH4NO3 in the MS culture medium of the proliferation medium is 1 / 2 to 1 / 3; the cutting method of tube seedling propagation comprises the following steps: dividing a test tube cluster seedling into 2-3 adventitious buds; removing the part which is 0.5cm over the basal part of a stem; and forming micro block mass and transferring to the proliferation medium for breeding. The defects that existing bamboo-root ginger easily generates low proliferation coefficient, vitrification and yellowing in a long-term in-vitro culture process are overcome; the method for improving the tissue culture, proliferation and seedling expansion effects of the bamboo-root ginger is high in proliferation coefficient; and vitrification and yellowing are effectively reduced.

Description

technical field [0001] The invention belongs to the technical field of tissue culture, and in particular relates to a method for improving the proliferation and robustness of bamboo root ginger tissue culture. Background technique [0002] Ginger is a perennial herbaceous plant belonging to Zingiberaceae Zingiberaceae. It is not only an important seasoning plant, but also has various medicinal effects such as relieving symptoms, dispelling cold and dampness, anti-tumor, and anti-oxidation. Because of its important medicinal and health value, it is favored by the market, and the planting area is increasing year by year. However, during the long-term asexual reproduction process of rhizomes, ginger has been generally infected by pathogens, resulting in species degradation, yield and quality reduction, seriously affecting the production and economic benefits of ginger. Virus-free ginger seedlings can be obtained in a short period of time by using shoot tip virus-free and tissu...

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to provide a high multiplication coefficient, effectively reduce the vitrification and yellowing shortcoming that the existing bamboo root ginger is prone to low multiplication coefficient, vitrification and yellowing in the long-term in vitro culture process. A method for improving the growth and growth of strong seedlings of bamboo root ginger tissue culture

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