A kind of high-temperature and dark culture tissue culture rapid propagation method of powder banana

A tissue culture fast propagation and dark culture technology, applied in the field of plant tissue culture, can solve the problem that cannot guarantee rapid and mass reproduction of healthy and high-quality pink banana seedlings, and reports on light and temperature regulation of pink banana tissue culture seedlings are few and increase the risk of variation. and other problems, to achieve the effect of improving space utilization, early germination, and shortening the generation cycle

Active Publication Date: 2018-06-22
广西壮族自治区农业科学院生物技术研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Increasing the concentration of phytohormones can increase the reproduction coefficient, but at the same time it will increase the risk of variation; while reducing the concentration of phytohormones can reduce the variation rate, but the reproduction coefficient will also decrease, which cannot guarantee rapid mass reproduction of healthy and high-quality banana seedlings
[0003] At present, some scholars have studied the effects of different temperatures and different light on the growth of banana tissue culture seedlings. The research shows that temperature has a very significant impact on the proliferation and rooting of banana tissue culture seedlings, but the effect of light and temperature regulation on powder banana tissue culture seedlings There are few related reports

Method used

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  • A kind of high-temperature and dark culture tissue culture rapid propagation method of powder banana
  • A kind of high-temperature and dark culture tissue culture rapid propagation method of powder banana
  • A kind of high-temperature and dark culture tissue culture rapid propagation method of powder banana

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for fast propagation of powdered bananas in high temperature and dark culture by tissue culture, comprising the following steps:

[0026] (1) Explant preparation and start-up culture: Collect 50cm high suction buds of the excellent mother tree of Fenbanana, rinse with tap water, cut off excess stems and leaves, scrub the surface with alcohol twice, peel off the leaf sheaths on the ultra-clean workbench and cut them A cylindrical stem with a diameter of 3 cm and a height of 5 cm with the growth point as the center is formed, and then a small square with a side length of 0.3 cm is peeled off from the center of the cylindrical stem, which is the explant small bud block, which is inoculated into the starting medium Cultivated for 25 days to obtain clump buds; the starting medium is MS medium+6-BA2.0mg / L+IBA0.2mg / L;

[0027] (2) Subculture; the clump buds that have been cultured are inoculated on the subculture medium, and cultivated under high temperature dark cult...

Embodiment 2

[0032] A method for fast propagation by tissue culture of powder banana in high temperature and dark culture, comprising the following steps:

[0033] (1) Explant preparation and start-up culture: Collect 100cm high suction buds of the excellent mother tree of Fenbanana, rinse with tap water, cut off excess stems and leaves, scrub the surface with alcohol 3 times, peel off the leaf sheaths on the ultra-clean workbench and cut them A cylindrical stem with a diameter of 3 cm and a height of 5 cm with the growth point as the center is formed, and then a small square with a side length of 0.5 cm is peeled off from the center of the cylindrical stem, which is the explant small bud block, which is inoculated into the starting medium Cultivated for 30 days to obtain clump buds; the starting medium is MS medium+6-BA3.0mg / L+IBA0.2mg / L;

[0034] (2) Subculture; the start-up cultured clump buds are inoculated on the subculture medium, and cultured for 30 days under high-temperature dark ...

Embodiment 3

[0039] A method for fast propagation by tissue culture of powder banana in high temperature and dark culture, comprising the following steps:

[0040] (1) Explant preparation and start-up culture: collect 80cm high suction buds of the excellent mother tree of Fenbanana, rinse with tap water, cut off excess stems and leaves, scrub the surface with alcohol for 3 times, peel off the leaf sheaths on the ultra-clean workbench and cut them A cylindrical stem with a diameter of 3 cm and a height of 5 cm with the growth point as the center is formed, and then a small square with a side length of 0.4 cm is peeled off from the center of the cylindrical stem, which is the explant small bud block, which is inoculated into the starting medium Cultured for 28 days to obtain clump buds; the starting medium was MS medium+6-BA2.5mg / L+IBA0.2mg / L;

[0041](2) Subculture; the clump buds that have been cultured are inoculated on the subculture medium, and cultured for 28 days under high temperatur...

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Abstract

The invention relates to the technical field of plant tissue culture, in particular to a method for high temperature dark culture tissue culture and rapid propagation of dwarf bananas. The method for high temperature dark culture tissue culture and rapid propagation of the dwarf bananas comprises the steps of explant preparation, subculture and rooting culture. According to the method, proliferation buds germinate early and perform differentiation early and quickly, the reproduction coefficient is high, and the effective bud rate is high; the percentage of seedling emergency of rooting seedlings is high, seedling blades and pseudostems are good in toughness, the root system does not drop easily, and the temporary planting survival rate is high. A high temperature dark culture technology is utilized, the subculture cycle is shortened by 10-15d, and the method has the advantages of reducing energy consumption, improving space utilization rate and production efficiency and reducing production cost.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a tissue culture and fast propagation method for high-temperature dark culture of powder banana. Background technique [0002] Pink banana (Musa ABB Pisang Awak), also known as Annan banana, Saigon banana and milk banana, and Banana (referred to as banana) (Musa AAA Cavendish) belong to two major types of staple bananas in my country. At present, the mainstream variety "Jinfen No. 1" on the market is favored by consumers because of its thin skin, pale yellow or yellow flesh, smooth flesh, sweet taste and slight fragrance. In recent years, the consumption of pink bananas has not only increased year by year in southern my country, but its market in the north has also begun to open, and the sales of pink bananas have gradually increased. With the expansion of the powder banana industry, high-quality and healthy powder banana seedlings are in urgent need of commercial p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/008
Inventor 邹瑜赵明龙芳何海旺武鹏林茜林贵美王趣有李林月吕朝安
Owner 广西壮族自治区农业科学院生物技术研究所
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