A kind of tissue culture rapid propagation method of fire dragon pearl

Abstract

In recent years, the market demand for dragon balls is relatively large and the economic benefits are good. They have been introduced and grown in many places in Yunnan and have begun to take shape. The conventional propagation method of dragon ball is mainly cutting, but its disadvantages are low reproduction coefficient, slow speed, long cycle, high cost, and easy to be affected by changes in the natural environment, etc., which directly affect the demand for seedlings and planting development of dragon ball. In order to solve the problems existing in the conventional propagation of pitaya seedlings, the present invention provides a tissue culture rapid propagation method of pitaya, including selection and collection of explants, explant disinfection, initiation and induction, proliferation and propagation, rooting and strong seedlings, etc. 5 steps. The technology solves the problems faced by the conventional propagation of dragon ball seedlings, and can achieve rapid, large-scale and efficient propagation of high-quality dragon ball tissue culture seedlings to meet the seedling needs of dragon ball planting and development, and has good social and economic benefits. application prospects.

Description

technical field

[0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a rapid propagation method of dragon pearl by tissue culture. Background technique

[0002] Fire Dragon Ball belongs to the Hypericum family, a low shrub of the genus Hypericum. Simple leaves, opposite or whorled, with glandular points, mostly entire; petals are 5, mainly yellow, with many stamens, usually connate. Pitaya is often used for cutting fruit or viewing flowers. The leaves are thick and papery, and the fruit is bright red and lustrous. The color of the fruit will not change after long-term storage, and it is delicate and lovely. It is an important ornamental plant.

[0003] In recent years, the market demand for fire dragon pearls has been relatively large, and the economic benefits have been good. They have been introduced and widely planted in many places in Yunnan, and have begun to take shape. The conventional propagation method of Fire D...

Examples

Embodiment 1

[0035] A kind of tissue culture rapid propagation method of fire dragon pearl, comprises the following steps:

[0036] A. Selective collection of explants: At noon on a sunny day, select the young shoots of the fire dragon pearl that are growing vigorously and free from diseases and insect pests as the collection objects. After the branches are intercepted, quickly put the branches into a bucket with a small amount of clear water and bring them back The explants are sterilized in the tissue culture room;

[0037] B. Sterilization of explants: Gently remove the petioles on the branches of Fire Dragon Ball in step A, cut off the branches into 1cm-long stems containing axillary buds, rinse the stems with running water for 0.5 minutes; Soak in fine water solution for 2 minutes; rinse with running water for 0.5 minutes after soaking, and finally sterilize with 75% alcohol for 30 seconds. After disinfection, sterilize the explants on an ultra-clean workbench;

[0038] C. Start-up i...

Embodiment 2

[0049] On the basis of embodiment 1, a kind of tissue culture rapid propagation method of fire dragon pearl, comprises the following steps:

[0050] A. Selective collection of explants: At noon on a sunny day, select the young shoots of the fire dragon pearl that are growing vigorously and free from diseases and insect pests as the collection objects. After the branches are intercepted, quickly put the branches into a bucket with a small amount of clear water and bring them back The explants are sterilized in the tissue culture room;

[0051] B. Sterilization of explants: Gently remove the petioles on the branches of Fire Dragon Ball in step A, cut off the branches into 1.5cm-long stems containing axillary buds, rinse the stems with running water for 1 minute; then wash with water Soak in fine water solution for 4 minutes; rinse with running water after soaking for 1 minute, and finally sterilize with 75% alcohol for 60 seconds. After disinfection, sterilize the explants on an...

Embodiment 3

[0063] On the basis of embodiment 1-2, a kind of tissue culture rapid propagation method of fire dragon pearl, comprises the following steps:

[0064] A. Selective collection of explants: At noon on a sunny day, select the young shoots of the fire dragon pearl that are growing vigorously and free from diseases and insect pests as the collection objects. After the branches are intercepted, quickly put the branches into a bucket with a small amount of clear water and bring them back The explants are sterilized in the tissue culture room;

[0065] B. Sterilization of explants: Gently remove the petioles on the branches of Fire Dragon Ball in step A, cut off the branches into 1-1.5cm long stems containing axillary buds, and rinse the stems with running water for 0.5-1min; Then soak in aqueous solution of detergent for 2-4 minutes; rinse with running water after soaking for 0.5-1 minute, and finally soak and disinfect with 75% alcohol for 30-60 seconds. Sterilize the explants;

...

technical field

[0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a rapid propagation method of dragon pearl by tissue culture. Background technique

[0002] Fire Dragon Ball belongs to the Hypericum family, a low shrub of the genus Hypericum. Simple leaves, opposite or whorled, with glandular points, mostly entire; petals are 5, mainly yellow, with many stamens, usually connate. Pitaya is often used for cutting fruit or viewing flowers. The leaves are thick and papery, and the fruit is bright red and lustrous. The color of the fruit will not change after long-term storage, and it is delicate and lovely. It is an important ornamental plant.

[0003] In recent years, the market demand for fire dragon pearls has been relatively large, and the economic benefits have been good. They have been introduced and widely planted in many places in Yunnan, and have begun to take shape. The conventional propagation method of Fire D...

Examples

Embodiment 1

[0035] A kind of tissue culture rapid propagation method of fire dragon pearl, comprises the following steps:

[0036] A. Selective collection of explants: At noon on a sunny day, select the young shoots of the fire dragon pearl that are growing vigorously and free from diseases and insect pests as the collection objects. After the branches are intercepted, quickly put the branches into a bucket with a small amount of clear water and bring them back The explants are sterilized in the tissue culture room;

[0037] B. Sterilization of explants: Gently remove the petioles on the branches of Fire Dragon Ball in step A, cut off the branches into 1cm-long stems containing axillary buds, rinse the stems with running water for 0.5 minutes; Soak in fine water solution for 2 minutes; rinse with running water for 0.5 minutes after soaking, and finally sterilize with 75% alcohol for 30 seconds. After disinfection, sterilize the explants on an ultra-clean workbench;

[0038] C. Start-up i...

Embodiment 2

[0049] On the basis of embodiment 1, a kind of tissue culture rapid propagation method of fire dragon pearl, comprises the following steps:

[0050] A. Selective collection of explants: At noon on a sunny day, select the young shoots of the fire dragon pearl that are growing vigorously and free from diseases and insect pests as the collection objects. After the branches are intercepted, quickly put the branches into a bucket with a small amount of clear water and bring them back The explants are sterilized in the tissue culture room;

[0051] B. Sterilization of explants: Gently remove the petioles on the branches of Fire Dragon Ball in step A, cut off the branches into 1.5cm-long stems containing axillary buds, rinse the stems with running water for 1 minute; then wash with water Soak in fine water solution for 4 minutes; rinse with running water after soaking for 1 minute, and finally sterilize with 75% alcohol for 60 seconds. After disinfection, sterilize the explants on an...

Embodiment 3

[0063] On the basis of embodiment 1-2, a kind of tissue culture rapid propagation method of fire dragon pearl, comprises the following steps:

[0064] A. Selective collection of explants: At noon on a sunny day, select the young shoots of the fire dragon pearl that are growing vigorously and free from diseases and insect pests as the collection objects. After the branches are intercepted, quickly put the branches into a bucket with a small amount of clear water and bring them back The explants are sterilized in the tissue culture room;

[0065] B. Sterilization of explants: Gently remove the petioles on the branches of Fire Dragon Ball in step A, cut off the branches into 1-1.5cm long stems containing axillary buds, and rinse the stems with running water for 0.5-1min; Then soak in aqueous solution of detergent for 2-4 minutes; rinse with running water after soaking for 0.5-1 minute, and finally soak and disinfect with 75% alcohol for 30-60 seconds. Sterilize the explants;

...

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