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Tissue culture rapid propagation method of hypericum

A technology of fire dragon and tissue culture, which is applied in the field of plant tissue culture, can solve the problems such as no fire dragon balls, and achieve good effect, small variation, good social and economic benefits and application prospects

Active Publication Date: 2021-01-12
玉溪市农业科学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] After literature search, there is no published content on the tissue culture of Huolongzhu

Method used

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  • Tissue culture rapid propagation method of hypericum
  • Tissue culture rapid propagation method of hypericum
  • Tissue culture rapid propagation method of hypericum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A kind of tissue culture rapid propagation method of fire dragon pearl, comprises the following steps:

[0036] A, selection collection of explants: at noon on a sunny day, select the young shoots of Fire Dragon Pearl that grow robustly and have no damage by diseases and insect pests as collection objects. Return to the tissue culture room for explant sterilization;

[0037] B. Sterilization of explants: Gently remove the petioles on the branches of Fire Dragon Ball in step A, cut off the branches into 1cm-long stems containing axillary buds, rinse the stems with running water for 0.5 minutes; Soak in fine water solution for 2 minutes; rinse with running water for 0.5 minutes after soaking, and finally sterilize with 75% alcohol for 30 seconds. After disinfection, sterilize the explants on an ultra-clean workbench;

[0038] C. Start-up induction: inoculate the sterilized stem segments in step B into the start-up induction medium for cultivation, the culture temperature...

Embodiment 2

[0049] On the basis of embodiment 1, a kind of tissue culture rapid propagation method of fire dragon pearl, comprises the following steps:

[0050] A, selection collection of explants: at noon on a sunny day, select the young shoots of Fire Dragon Pearl that grow robustly and have no damage by diseases and insect pests as collection objects. Return to the tissue culture room for explant sterilization;

[0051] B. Sterilization of explants: Gently remove the petioles on the branches of Fire Dragon Ball in step A, cut off the branches into 1.5cm-long stems containing axillary buds, rinse the stems with running water for 1 minute; then wash with water Soak in fine water solution for 4 minutes; rinse with running water after soaking for 1 minute, and finally sterilize with 75% alcohol for 60 seconds. After disinfection, sterilize the explants on an ultra-clean workbench;

[0052] C. Start-up induction: inoculate the sterilized stem segments in step B into the start-up induction ...

Embodiment 3

[0063] On the basis of embodiment 1-2, a kind of tissue culture rapid propagation method of fire dragon pearl, comprises the following steps:

[0064] A, selection collection of explants: at noon on a sunny day, select the young shoots of Fire Dragon Pearl that grow robustly and have no damage by diseases and insect pests as collection objects. Return to the tissue culture room for explant sterilization;

[0065] B. Sterilization of explants: Gently remove the petioles on the branches of Fire Dragon Ball in step A, cut off the branches into 1-1.5cm long stems containing axillary buds, and rinse the stems with running water for 0.5-1min; Then soak in aqueous solution of detergent for 2-4 minutes; rinse with running water after soaking for 0.5-1 minute, and finally soak and disinfect with 75% alcohol for 30-60 seconds. Sterilize the explants;

[0066] C. Start-up induction: inoculate the sterilized stem segments in step B into the start-up induction medium for cultivation, the...

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Abstract

In recent years, the market demand of hypericum is large, economic benefits are better, the hypericum is introduced and planted in many places in Yunnan, and a certain scale is initially achieved. A conventional propagation method of the hypericum mainly adopts cuttage, but has the defects of low propagation coefficient, low speed, a long period, high cost, high possibility of being influenced bynatural environment change and the like, and directly influences the seedling requirements and planting development of the hypericum. In order to solve the problems existing in conventional propagation of hypericum seedlings, the invention provides a tissue culture rapid propagation method of the hypericum. The method comprises the following five steps of selection and collection of explants, disinfection of the explants, induction starting, proliferation and propagation expanding, rooting and seedling strengthening, and the like. According to the technology, the problems faced by conventionalpropagation of the hypericum seedlings are solved, the high-quality hypericum tissue culture seedlings can be propagated rapidly, massively and efficiently, the seedling requirements of hypericum planting development are met, and the method has well social and economic benefits and application prospects.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a rapid propagation method of dragon pearl by tissue culture. Background technique [0002] Fire Dragon Ball belongs to the Hypericum family, a low shrub of the genus Hypericum. Simple leaves, opposite or whorled, with glandular points, mostly entire; petals are 5, mainly yellow, with many stamens, usually connate. Pitaya is often used for cutting fruit or viewing flowers. The leaves are thick and papery, and the fruit is bright red and lustrous. The color of the fruit will not change after long-term storage, and it is delicate and lovely. It is an important ornamental plant. [0003] In recent years, the market demand for fire dragon pearls has been relatively large, and the economic benefits have been good. They have been introduced and widely planted in many places in Yunnan, and have begun to take shape. The conventional propagation method of Fire D...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李晓亮杨世先张军云杨光炤张钟王文智张建康钱遵姚董广陈佳清
Owner 玉溪市农业科学院
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