Detoxification and rapid propagation method of snow white strawberries

A technology of Snow White and strawberry, which is applied in the field of agricultural seedling raising, can solve the problems of poisonous seedlings and slow propagation of seedlings, and achieves the effects of developed root system, healthy and tidy individual growth, and improved proliferation rate.

Inactive Publication Date: 2018-08-31
河北富硕农业科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies in the prior art, the present invention provides a method for detoxification and rapid propagation of Snow White strawberry, which uses stolons as stem tip stripping tissues, and breeds detoxification strawberry seedlings through tissue culture technology, so as to solve the problem of the actual production process of Snow White strawberries. The problem of slow propagation of seedlings and poisonous seedlings can improve the yield and quality of Snow White strawberry. The virus-free seedlings of Snow White strawberry cultivated have the advantages of strong plant growth, well-developed root system, strong and neat individual growth, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] S1. Selection of explants and surface cleaning: select pure and healthy Snow White strawberry grape stem tip 2-4 cm without pests and insects as explants, wash once with detergent, and then wash 2-3 times with tap water , and then washed once with Tween, and then rinsed with running tap water for 20-30 minutes;

[0036] S2. Sterilization and disinfection: place the explants cleaned on the surface of step S1 on the ultra-clean workbench, first wash them twice with sterile water, then sterilize them with 75% alcohol for 30 seconds, and then sterilize them with 0.1% mercuric chloride for 3 minutes , constantly stirring during the whole sterilization process, the stirring speed is 150r / min, then rinse 5 times with sterile water, and the explants are sterilized;

[0037] S3. Induction culture: under a dissecting microscope, use a dissecting needle to pick out 0.5 mm of the shoot tip of the explant, and inoculate it on the induction medium. The induction medium is Ms+6-BA 0.4...

Embodiment 2

[0042] S1. Selection of explants and surface cleaning: select pure and healthy Snow White strawberry grape stem tip 2-4 cm without pests and insects as explants, wash once with detergent, and then wash 2-3 times with tap water , and then washed once with Tween, and then rinsed with running tap water for 20-30 minutes;

[0043] S2. Sterilization and disinfection: place the explants cleaned on the surface of step S1 on the ultra-clean workbench, first wash them twice with sterile water, then sterilize them with 75% alcohol for 30 seconds, and then sterilize them with 0.1% mercuric chloride for 3 minutes , constantly stirring during the whole sterilization process, the stirring speed is 150r / min, then rinse 5 times with sterile water, and the explants are sterilized;

[0044] S3. Induction culture: under a dissecting microscope, use a dissecting needle to pick out 0.5 mm of the shoot tip of the explant, and inoculate it on the induction medium. The induction medium is Ms+6-BA0.4m...

Embodiment 3

[0049] S1. Selection of explants and surface cleaning: select pure and healthy Snow White strawberry grape stem tip 2-4 cm without pests and insects as explants, wash once with detergent, and then wash 2-3 times with tap water , and then washed once with Tween, and then rinsed with running tap water for 20-30 minutes;

[0050] S2. Sterilization and disinfection: place the explants cleaned on the surface of step S1 on the ultra-clean workbench, first wash them twice with sterile water, then sterilize them with 75% alcohol for 30 seconds, and then sterilize them with 0.1% mercuric chloride for 3 minutes , constantly stirring during the whole sterilization process, the stirring speed is 150r / min, then rinse 5 times with sterile water, and the explants are sterilized;

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PUM

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Abstract

The invention relates to a detoxification and rapid propagation method of snow white strawberries. The detoxification and rapid propagation method comprises the following steps: S1, explant selectionand surface cleaning, namely selecting 2-4cm stem tips of snow white strawberry grape stems as explants, and carrying out surface cleaning; S2, sterilization, namely placing the surface-cleaned explants on a workbench for sterilization; S3, induced cultivation, namely picking 0.5mm stem tips of the explants by using a dissecting needle under an anatomical lens, and inoculating the 0.5mm stem tipson an induced culture medium for induced culture for 50-60 days to obtain young seedlings of the snow white strawberries; S4, virus detection and seedling selection, namely carrying out virus detection on the young seedlings of the snow white strawberries by using a virus kit, and selecting the young seedlings, reaching the standard, of the snow white strawberries; S5, subculture multiplication culture, namely transplanting the young seedlings, reaching the standard, of the snow white strawberries into a multiplication culture medium, and carrying out subculture once every 30 days for 5-6 times; and S6, rooting culture, namely transplanting young seedlings with the plant heights being 3-5cm of the snow white strawberries into a rooting culture medium for culture for 20-30 days to obtain snow white strawberry plants.

Description

technical field [0001] The invention belongs to the technical field of agricultural seedling cultivation, and relates to a method for detoxification and rapid propagation of Snow White strawberries. Background technique [0002] Strawberry belongs to Rosaceae Strawberry is a perennial herb and is an important economic crop. Strawberry pulp contains a lot of nutrients such as protein, sugar and pectin. Its vitamin C content is higher than that of apples, grapes and other fruits. It tastes sweet and sour and delicious, and is very popular among people. In addition to eating fresh, strawberries can be processed into products such as canned food, jam, fruit wine and beverages. In recent years, my country's strawberry production has developed rapidly, and the area and output of strawberries have leapt to the first place in the world, playing an important role in promoting agricultural efficiency and increasing farmers' income in various places. The traditional method of strawbe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 张建盈郭玲娟张立田史晋鹏张彦强赵志伟
Owner 河北富硕农业科技发展有限公司
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