Detoxification and rapid propagation method of snow white strawberries
A technology of Snow White and strawberry, which is applied in the field of agricultural seedling raising, can solve the problems of poisonous seedlings and slow propagation of seedlings, and achieves the effects of developed root system, healthy and tidy individual growth, and improved proliferation rate.
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Embodiment 1
[0035] S1. Selection of explants and surface cleaning: select pure and healthy Snow White strawberry grape stem tip 2-4 cm without pests and insects as explants, wash once with detergent, and then wash 2-3 times with tap water , and then washed once with Tween, and then rinsed with running tap water for 20-30 minutes;
[0036] S2. Sterilization and disinfection: place the explants cleaned on the surface of step S1 on the ultra-clean workbench, first wash them twice with sterile water, then sterilize them with 75% alcohol for 30 seconds, and then sterilize them with 0.1% mercuric chloride for 3 minutes , constantly stirring during the whole sterilization process, the stirring speed is 150r / min, then rinse 5 times with sterile water, and the explants are sterilized;
[0037] S3. Induction culture: under a dissecting microscope, use a dissecting needle to pick out 0.5 mm of the shoot tip of the explant, and inoculate it on the induction medium. The induction medium is Ms+6-BA 0.4...
Embodiment 2
[0042] S1. Selection of explants and surface cleaning: select pure and healthy Snow White strawberry grape stem tip 2-4 cm without pests and insects as explants, wash once with detergent, and then wash 2-3 times with tap water , and then washed once with Tween, and then rinsed with running tap water for 20-30 minutes;
[0043] S2. Sterilization and disinfection: place the explants cleaned on the surface of step S1 on the ultra-clean workbench, first wash them twice with sterile water, then sterilize them with 75% alcohol for 30 seconds, and then sterilize them with 0.1% mercuric chloride for 3 minutes , constantly stirring during the whole sterilization process, the stirring speed is 150r / min, then rinse 5 times with sterile water, and the explants are sterilized;
[0044] S3. Induction culture: under a dissecting microscope, use a dissecting needle to pick out 0.5 mm of the shoot tip of the explant, and inoculate it on the induction medium. The induction medium is Ms+6-BA0.4m...
Embodiment 3
[0049] S1. Selection of explants and surface cleaning: select pure and healthy Snow White strawberry grape stem tip 2-4 cm without pests and insects as explants, wash once with detergent, and then wash 2-3 times with tap water , and then washed once with Tween, and then rinsed with running tap water for 20-30 minutes;
[0050] S2. Sterilization and disinfection: place the explants cleaned on the surface of step S1 on the ultra-clean workbench, first wash them twice with sterile water, then sterilize them with 75% alcohol for 30 seconds, and then sterilize them with 0.1% mercuric chloride for 3 minutes , constantly stirring during the whole sterilization process, the stirring speed is 150r / min, then rinse 5 times with sterile water, and the explants are sterilized;
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