A kind of subculture medium formula of pearl cassia osmanthus tissue culture seedlings and its application

The technology of a subculture medium and cassia group is applied in the field of subculture medium formula of pearl cassia tissue culture seedlings, which can solve the problems of low survival rate of grafting, difficult reproduction of sweet-scented osmanthus, low reproduction coefficient and the like, and achieve the effect of promoting proliferation. , the differentiation stability, the effect of increasing the proliferation coefficient

Inactive Publication Date: 2021-09-21
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

However, if the cutting propagation is carried out by the general method, the survival rate is very low; air pressure and ground pressure are commonly used for layering, although the survival rate is high, the reproduction coefficient is very low; with the grafting method, the grafting survival rate is not high. The source of the above article is limited, so the development is limited to a certain extent
[0004] In recent years, rapid propagation technology has been widely used in the field of agricultural technology, but for each variety of plant, the technical methods and nutrient composition formulas vary widely, and there is no report on the rapid propagation of pearl cassia osmanthus by using tissue culture technology.

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  • A kind of subculture medium formula of pearl cassia osmanthus tissue culture seedlings and its application
  • A kind of subculture medium formula of pearl cassia osmanthus tissue culture seedlings and its application

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Embodiment Construction

[0014] 1. Test material: the axillary buds induced by the explants were used as the test material, the axillary buds were cut off on the ultra-clean workbench, inoculated into the subculture medium, and the statistics were observed.

[0015] 2. Subculture: The subculture of Pearl Caigui tissue culture seedlings uses woody plant medium WPM, using L 9 (3 4 ) Orthogonal test design (see Table 1), cytokinin 6-BA set 3 levels (final concentration): 3.0 mg L -1 , 4.0 mg·L -1 and 5.0 mg·L -1 . Kinetin KT setting 3 level (final concentration): 1.0 mg·L -1 , 2.0 mg·L -1 and 3.0 mg·L -1 . Gibberellin GA 3 Set 3 levels (final concentration): 0.1 mg L -1 , 0.3 mg·L -1 , 0.5 mg·L -1 . The proliferation coefficient was counted 25 days after inoculation.

[0016] The preparation method of woody plant medium WPM is as follows: Weigh 29.6g of WPM and culture it in 1L of distilled water, heat and boil until completely dissolved.

[0017] Table 1 Factors and level setting of proli...

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Abstract

The present invention relates to a formulation of subculture medium for tissue cultured seedlings of Pearl Color Osmanthus and its application. The components of the medium include woody plant medium WPM, cytokinin 6-BA, kinetin KT and gibberellin GA 3 , including a final concentration of 6.5g·L ‑1 agar and a final concentration of 30g·L ‑1 sucrose; after analysis, the best medium for the proliferation of Zhenzhu Caigui is: woody plant medium WPM, final concentration 4.0 mg·L ‑1 Cytokinin 6-BA, final concentration 2.0 mg·L ‑1 Kinetin KT, final concentration 0.5 mg·L ‑1 gibberellin GA3, final concentration 6.5g L ‑1 Agar, final concentration 30g L ‑1 sucrose; under this condition, the multiplication coefficient of Pearl Caigui tissue culture seedlings can reach 5.8.

Description

technical field [0001] The invention belongs to the field of culturing pearl cassia tissue-cultured seedlings, and in particular relates to a formulation of a subculture medium for pearl cassia tissue-cultured seedlings and an application thereof. Background technique [0002] Pearl color osmanthus is a variant of Osmanthus osmanthus. The leaves are oval and serrated, and the leaves are thicker than ordinary green-leaved osmanthus. The leaves can change color with the seasons. The buds in early spring are purplish red, then pink, and then golden yellow to white. Then from the central main vein, it gradually changes to green on both sides. Until the dark green, under the condition of suitable climate, there are buds emerging all the year round, so the whole bead plant is colorful and colorful from top to bottom. [0003] The propagation of Pearl Caigui generally adopts methods such as cutting, layering and grafting. However, if the cutting propagation is carried out by th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001A01H4/008
Inventor 孙红英辛全伟罗海凌严少娟
Owner FUJIAN AGRI & FORESTRY UNIV
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