A kind of tissue culture method of North American holly

A technology of tissue culture and holly, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low reproduction coefficient and achieve the effects of high survival rate, pure quality, and strong branches

Active Publication Date: 2015-10-21
HANGZHOU LANDSCAPING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using this method to propagate North American holly, the multiplication rate can reach 6.1, and the rooting rate can reach 95.2%, successfully solving the problem of low reproduction coefficient of North American holly in the existing technology,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] On the morning of June 13, 2012, healthy and typical ornamental plants were selected from the female seedlings of North American holly, and the semi-lignified branches of the year were collected. Cut off the leaves after collection, while retaining the 1.2cm petiole, rinse with tap water, soak in detergent solution for 6 minutes, and then rinse with tap water for 1 hour for later use; on the ultra-clean workbench, cut the cleaned branches into 2.5cm single Buds and stems are disinfected sequentially, soaked in 75% alcohol solution for 30 seconds, rinsed with sterile water for 5 times, soaked in 1‰ mercury liter solution for 6 minutes, and shaken continuously to make the disinfection complete, and finally rinsed with sterile water for 8 times; then Use a sterile knife to cut off the 0.2cm dead tissue at the upper and lower ends of the stem segment, and insert the treated stem segment plant morphological lower end into the prepared induction differentiation medium: the for...

Embodiment 2

[0029] On the morning of May 14, 2013, healthy and typical ornamental plants were selected from the female seedlings of North American holly, and the semi-lignified branches of the year were collected. Cut off the leaves after collection, while retaining the 1.0cm petiole, rinse with tap water, soak in detergent solution for 8 minutes, and then rinse with tap water for 1 hour for later use; on the ultra-clean workbench, cut the cleaned branches into 2.2cm single buds The stems are soaked in 75% alcohol solution for 30s in turn, after pouring off the alcohol, rinse with sterile water for 5 times, then add 1‰ mercury liter solution to soak for 6 minutes, shake continuously during this period, pour out the mercury liter solution and rinse with sterile water 8 times for standby; then use a sterile knife to cut off the 0.25cm dead tissue at the upper and lower ends of the stem segment, and insert the lower end of the stem segment plant morphology into the prepared differentiation in...

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PUM

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Abstract

The invention provides a tissue culture method of ilex verticillata. The tissue culture method comprises the following steps: (1) selecting an explant from a female plant; (2) processing the explant, namely sterilizing the explant; (3) performing inducing culturing on buds; (4) culturing the buds in enrichment; and (5) performing inducing culturing on roots. The tissue culture method disclosed by the invention is high in survival rate and high in reproduction speed, the growth coefficient achieves 6.1, the rooting percentage achieves 95.2%, and the ilex verticillata female young plant with pure quality is obtained. The method is free from season limitations, and can be used for rapidly propagating the ilex verticillata throughout the year, so that the large production of the ilex verticillata is realized, and an available method is provided for the rapid extension of the ilex verticillata.

Description

technical field [0001] The invention belongs to the technical field of plant culture, in particular to a tissue culture method suitable for North American holly. Background technique [0002] North American holly (Ilex verticillata), also known as whorling holly and American holly, is a perennial shrub of the genus Ilex (Ilexlinn.) in the Ilex family. Native to the Northeast of the United States, it is a newly introduced variety in Zhejiang Province. It has been found through introduction and cultivation that the plant has strong resistance and wide adaptability, and the red fruit does not fall for a long time in winter. It is an excellent ornamental tree species for cutting branches in autumn and winter, beautifying the environment and potting. [0003] At present, North American holly is only propagated by seeds and cuttings. If seed propagation is adopted, the traits of the progeny are separated greatly, and the excellent traits of the female parent cannot be maintained,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 张俊林钱飞孙英坤
Owner HANGZHOU LANDSCAPING
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