Tissue culture rapid propagation method of Guangdong anoectochilus roxburghii

Abstract

The invention discloses a tissue culture rapid propagation method of Guangdong anoectochilus roxburghii, which selects an axillary bud or a terminal bud of anoectochilus roxburghii in the Guangdong area in China as an explant and obtains a mass of vegetative propagated progeny plants by inducing the adventitious buds, carrying out proliferation and carrying out rooting cultivation. By adopting the method of the invention, one axillary bud can be induced to generate about 2 to 4 sprouts, after two months, the proliferation coefficient is between 3 and 4, and the rooting rate can reach over 90 percent. Therefore, the method greatly improves the seedling production capacity of Guangdong anoectochilus roxburghii, shortens the seedling culture time, saves the planting cost and provides the technical guarantee for the storage of the genetic resources of Guangdong anoectochilus roxburghii.

Description

technical field

[0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for tissue culture and rapid propagation of Aureus aureus in Guangdong. Background technique

[0002] Anoectochitus formosanus (Anoectochitus formosanus) is a traditional precious medicinal material in my country. It belongs to the perennial herb of the family Orchidaceae. Golden clematis can also be used as an ornamental plant with high ornamental value. Auranthus clematis is rich in vitamins and polysaccharides, with high content of trace elements and amino acids, and has antibacterial, anticancer, cardiotonic, analgesic, and hypoglycemic effects. Due to the good efficacy of clematis in the treatment of liver disease, hypertension, diabetes and tumors, it has increasingly attracted the attention of the medical community.

[0003] But the clematis seeds are tiny, the germination rate is low, its growth has strict requirements on the ecological...