Method for performing tissue culture of dendrobium candidum seedlings

A technology of Dendrobium officinale and tissue culture, applied in the field of plant biology, can solve the problems of low reproduction rate, difficult mass production and industrial production, slow differentiation, etc., and achieve the effects of promoting rapid growth, easy management, and less pests and diseases

Inactive Publication Date: 2015-04-29
ANHUI KANGJIU BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods optimize the medium components of traditional production, are easy to operate, low in production cost, and have medicinal ingredients close to those of wild Dendrobium officinale, they have problems such as low reproduction rate, slow differentiation, and difficulty in mass production and factory production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A kind of method for dendrobium candidum seedling tissue culture that the present invention proposes, comprises the steps:

[0024] S1. Sterilization: UV sterilize the inoculation room and inoculation instruments;

[0025] S2, germination: select qualified capsules of Dendrobium officinale, disinfect the capsules, soak the surface of the capsules with 95% medical alcohol, cut the disinfected capsules longitudinally, take out the seeds with tweezers and evenly scatter them in a container filled with germination nutrient solution. Seed germination culture is carried out in the culture bottle, the culture temperature is 23°C, the culture light intensity is 1800Lux, the culture light time is 12h / d, and the average disinfection frequency is 1.5 times / day. After 35 days of culture, green protocorms are formed, and the germination nutrient solution The components include: manganese sulfate 0.012g / L, zinc sulfate 6.2×10 -3 g / L, copper sulfate 5.4×10 -5g / L, ferrous sulfate 0.0...

Embodiment 2

[0028] A kind of method for dendrobium candidum seedling tissue culture that the present invention proposes, comprises the steps:

[0029] S1. Sterilization: Ozone sterilizes the inoculation room and inoculation equipment;

[0030] S2, germination: select qualified capsules of Dendrobium officinale, disinfect the capsules, soak the surface of the capsules with 95% medical alcohol, cut the disinfected capsules longitudinally, take out the seeds with tweezers and evenly scatter them in a container filled with germination nutrient solution. Seed germination culture is carried out in the culture bottle, the culture temperature is 24°C, the culture light intensity is 1500Lux, the culture light time is 13h / d, and the average disinfection frequency is 1 time / day. After 40 days of culture, green protocorms are formed, and the germination nutrient solution The components include: manganese sulfate 0.007g / L, zinc sulfate 6.4×10 -3 g / L, copper sulfate 5.3×10 -5 g / L, ferrous sulfate 0.0...

Embodiment 3

[0033] A kind of method for dendrobium candidum seedling tissue culture that the present invention proposes, comprises the steps:

[0034] S1. Sterilization: UV sterilize the inoculation room, and ozone sterilize the inoculation equipment;

[0035] S2, germination: select qualified capsules of Dendrobium officinale, disinfect the capsules, soak the surface of the capsules with 95% medical alcohol, cut the disinfected capsules longitudinally, take out the seeds with tweezers and evenly scatter them in a container filled with germination nutrient solution. Seed germination culture is carried out in the culture bottle, the culture temperature is 23°C, the culture light intensity is 1600Lux, the culture light time is 12h / d, and the average disinfection frequency is 1.4 times / day. Green protocorms are formed after 38 days of culture, and the germination nutrient solution The components include: manganese sulfate 0.01g / L, zinc sulfate 6.30×10 -3 g / L, copper sulfate 5.36×10 -5 g / L,...

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Abstract

The invention discloses a method for performing tissue culture of dendrobium candidum seedlings. The method comprises the following steps: sterilizing: sterilizing an inoculation chamber and inoculators; germinating: selecting dendrobium candidum capsules, disinfecting the capsules, longitudinally cutting up the capsules, taking out seeds by a pair of tweezers, uniformly spreading the seeds in a culture bottle containing germination nutrient solution to perform seed germination culture for 35-40 days, and forming green protocorms; transplanting: moving the protocorms in a culture bottle containing tissue culture nutrient solution to perform protocorm proliferation and differentiation culture for 20-25 days, and obtaining young seedlings; moving the young seedlings to a culture bottle containing the tissue culture nutrient solution to perform seedling proliferation and differentiation culture for 30-35 days, and obtaining plantlets; moving the plantlets to a culture bottle containing the tissue culture nutrient solution to perform strong seedling culture for 60-65 days, and obtaining middle-age seedlings; moving the middle-age seedlings to a culture bottle containing the tissue culture nutrient solution to perform strong seedling culture for 65-70 days, and obtaining the dendrobium candidum seedlings.

Description

technical field [0001] The invention relates to the field of plant biotechnology, in particular to a method for tissue culture of dendrobium officinale seedlings. Background technique [0002] Dendrobium candidum is a well-known species of Dendrobium, which has unique medicinal value and ranks first among the nine Chinese immortal grasses. The "Shen Nong's Materia Medica" in the Qin and Han Dynasties records that Dendrobium candidum "mainly injures, eliminates numbness, lowers qi, nourishes the five viscera due to fatigue and emaciation, strengthens yin, and thickens the stomach after long-term use." Modern chemical and pharmacological studies have shown that the main active ingredient of Dendrobium officinale is polysaccharide, and the polysaccharide content is as high as 20-30%. Because of its polysaccharides, alkaloids, amino acids, trace elements and other ingredients contained in Dendrobium can enhance the body's immune ability, anti-oxidation, anti-aging, lower blood ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李军
Owner ANHUI KANGJIU BIOTECH
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