A method for improving regeneration efficiency during camphor tissue culture

Abstract

The invention provides a method for improving regeneration efficiency in the tissue culture process of Cinnamomum camphora. First, sterilize the seeds of Cinnamomum camphora, and germinate the sterilized seeds to obtain aseptic Cinnamomum camphora seedlings; then, use the aseptic Cinnamomum camphora seedlings As explants, the corresponding Cinnamomum camphora tissue culture method is established through the steps of "induction of buds", "proliferation of buds" and "rooting of buds"; wherein, in the step of "proliferation of buds" Activated carbon was added as an additional component in the "propagation medium" used and the "rooting medium" used in the "rooting of shoots" step. The present invention has the advantages that: (1) active carbon is added in part of the culture medium to absorb metabolites, so that the value-added coefficient of tissue culture seedlings is increased, the browning rate is reduced, and the rooting rate is increased, thereby improving the regeneration efficiency of camphor tissue culture; (2) Choose to sterilize the seeds to effectively avoid the damage caused by the "disinfection process" to the young and tender stems of the materials required in subsequent experiments.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for improving regeneration efficiency in the tissue culture process of camphor camphora. Background technique [0002] Camphora (Cinnamomum caphora), belonging to the genus Lauraceae, has a majestic tree shape, a tall and straight trunk, evergreen in all seasons, a large crown and thick shade, beautiful and fragrant branches and leaves, and is a very beautiful and excellent street tree in southern cities in my country. It is one of the indispensable tree species to form a plant gardening landscape. [0003] At present, camphor seedlings are mainly raised in three ways. The first is to grow seedlings, which is the main way of cultivating camphor seedlings; however, there are disadvantages such as strong seasonality and difficulty in retaining good characters. The second is to grow seedlings by cuttings, but it needs more ear picking mother plants. At the same t...

AI Technical Summary

Problems solved by technology

The first is to grow seedlings, which is the main way of cultivating camphor seedlings; however, there are disadvantages such as strong seasonality and difficulty in retaining excellent traits in seedlings

The second is to grow seedlings by cuttings, but it needs more ear picking mother plants. At the same time, the cuttings are greatly affected by environmental conditions, and the rooting effect is unstable; the third is tissue culture technology, which is most suitable for rapid propagation when the female parent material is limited. technology

[0004] However, during the tissue culture process of Cinnamomum camphora, due to the accumulation of various metabolites in its own growth, the plants themselves often have low proliferation coefficient, difficulty in rooting, and high browning rate. Therefore, the normal growth of Cinnamomum camphora plants will be affected to varying degrees. Influenced, the plant regeneration efficiency during the tissue culture of Cinnamomum camphora is not guaranteed

Images