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A kind of peach fruit callus culture medium and culture method thereof

A technology of callus and culturing method, applied in the field of peach fruit callus medium and its culture, can solve the problems of low success rate of callus culture, inconsistent callus state, etc., and achieve stable traits and maintain consistency , highly consistent effect

Active Publication Date: 2018-10-12
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Purpose of the invention: Aiming at the problems existing in the prior art, the present invention provides a peach fruit callus culture medium and a culture method thereof; the present invention greatly improves the peach fruit callus induction rate; by cultivating the peach pulp callus can Obtain a large number of high-quality callus in a short period of time, solve the problems of inconsistent callus status and low success rate of callus culture in peach leaf culture, provide an effective way for large-scale culture of callus, and contribute to the molecular biology and Genetic engineering research offers a new avenue

Method used

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  • A kind of peach fruit callus culture medium and culture method thereof
  • A kind of peach fruit callus culture medium and culture method thereof
  • A kind of peach fruit callus culture medium and culture method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Medium:

[0028] Modified WPM, TDZ1mg / L, 2,4-D 0.2mg / L, 6-BA 0.2mg / L, sucrose 20g / L and agar powder 4.5g / L, pH 5.5;

[0029] The improved WPM medium contains for every liter of WPM medium: KNO 3 Potassium nitrate 300mg / L, K 2 SO 4 Potassium sulfate 800mg / L, MgSO 4 .7H 2 O magnesium sulfate heptahydrate 350mg / L, KH 2 PO 4 Potassium dihydrogen phosphate 150mg / L, MnSO 4 .4H 2 O manganese sulfate tetrahydrate 15mg / L, ZnSO 4 .7H 2 O zinc sulfate heptahydrate 8.0mg / L, H 3 BO 3 Boric acid 6.0mg / L, CuSO 4 .5H 2 O copper sulfate pentahydrate 0.03mg / L, NaMoO 4 .2H 2 O dihydrate sodium molybdate 0.1mg / L, Fe-EDDHA ethylenediamine o-diglycolic acid iron 8mg / L, CaCl 2 .2H 2 Calcium chloride O dihydrate 80mg / L, Ca(NO 3 ) 2 .4H 2 O calcium nitrate tetrahydrate 550mg / L, inositol 80mg / L, niacin 0.8mg / L, vitamin B 1 0.8mg / L, vitamin B 6 1.5mg / L, Vitamin C 1.5mg / L.

[0030] Training method:

[0031] (1) Aseptic treatment of peach fruit: select peach fruit 30 days a...

Embodiment 2

[0034] Medium:

[0035] Improved WPM, 2,4-D 2mg / L, ZT 3mg / L, 6-BA 0.8mg / L, sucrose 40g / L, agar powder 6.5g / L, pH 6.0;

[0036] The improved WPM medium contains for every liter of WPM medium: KNO 3 Potassium nitrate 500mg / L, K 2 SO 4 Potassium sulfate 1000mg / L, MgSO 4 .7H 2 O magnesium sulfate heptahydrate 400mg / L, KH 2 PO 4 Potassium dihydrogen phosphate 200mg / L, MnSO 4 .4H 2 O manganese sulfate tetrahydrate 25mg / L, ZnSO 4 .7H 2 O zinc sulfate heptahydrate 9.0mg / L, H 3 BO 3 Boric acid 6.5mg / L, CuSO 4 .5H 2 O copper sulfate pentahydrate 0.08mg / L, NaMoO 4 .2H 2 O dihydrate sodium molybdate 0.4mg / L, Fe-EDDHA ethylenediamine o-diglycolic acid iron 12mg / L, CaCl 2 .2H 2 Calcium chloride O dihydrate 120mg / L, Ca(NO 3 ) 2 .4H 2 O calcium nitrate tetrahydrate 650mg / L, inositol 120mg / L, niacin 1.2mg / L, vitamin B 1 1.2mg / L, vitamin B 6 2.5mg / L, Vitamin C 2.5mg / L.

[0037] Training method:

[0038] (1) Aseptic treatment of peach fruits: select peach fruits 70 days ...

Embodiment 3

[0041] Medium:

[0042] Improved WPM, ZT2.0mg / L, 2,4-D0.2mg / L, IBA0.2mg / L, 30g / L sucrose, 5.5g / L agar powder, pH 5.8;

[0043] The improved WPM medium contains for every liter of WPM medium: KNO 3 Potassium nitrate 400mg / L, K 2 SO 4 Potassium sulfate 900mg / L, MgSO 4 .7H 2 O magnesium sulfate heptahydrate 370mg / L, KH 2 PO 4 Potassium dihydrogen phosphate 170mg / L, MnSO 4 .4H 2 O manganese sulfate tetrahydrate 20mg / L, ZnSO 4 .7H 2 O zinc sulfate heptahydrate 8.6mg / L, H 3 BO 3 Boric acid 6.2mg / L, CuSO 4 .5H 2 O copper sulfate pentahydrate 0.05mg / L, NaMoO 4 .2H 2 O dihydrate sodium molybdate 0.25mg / L, Fe-EDDHA ethylenediamine o-diglycolic acid iron 10mg / L, CaCl 2 .2H 2 Calcium chloride O dihydrate 100mg / L, Ca(NO 3 ) 2 .4H 2 O calcium nitrate tetrahydrate 600mg / L, inositol 100mg / L, niacin 1mg / L, vitamin B 1 1mg / L, vitamin B 6 2mg / L, Vitamin C 2mg / L.

[0044] Training method:

[0045] (1) Aseptic treatment of peach fruits: select peach fruits 50 days after t...

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Abstract

The invention discloses a peach fruit callus tissue culture medium and a culture method thereof. The culture medium comprises the following components: an improved WPM culture medium as a basic culture medium, a plant-growth regulator, 20-40 g / L of cane sugar, and 4.5-6.5 g / L of agar powder, wherein the pH value is 5.5-6.0. The culture method comprises the following steps: 1) bacteria-free treatment on peach fruits, and 2) round slice culture on the peach fruits. The callus induction rate of the peach fruits is greatly increased by the callus tissue culture medium and the culture method; a lot of high-quality callus tissues can be obtained in a short period by culturing the callus tissues of the peach pulp, problems of inconsistent callus tissue states in culture of peach tree leaves and low success rate of callus tissue culture can be solved, the method provides an effective approach for large-scale culture of the callus tissue, and provides a novel approach for molecular biology and gene engineering research of peaches.

Description

technical field [0001] The invention relates to plant callus culture, in particular to a peach fruit callus culture medium and a culture method thereof. Background technique [0002] Peach originates in my country, is widely cultivated in various provinces and autonomous regions of my country at present, and also all has plantation all over the world. Peach is a fruit tree with nutritional value, economic value and ornamental value. At present, research on the peach genome has also begun in my country. The research direction of the peach genome mainly involves several major aspects such as plant resistance, fruit quality and fruit production characteristics. Among them, the important economic traits of peach, especially the molecular mechanism related to the formation of fruit quality It has also become a hot spot in peach genome research. In the molecular biology research of peach, a major problem that has troubled researchers for many years is the transgene of peach. At p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001A01H4/005
Inventor 王媛花蔡善亚史红林
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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