83 results about "Gerbera jamesonii" patented technology

The invention discloses a method for rapid identification of Gerbera jamesonii ploidy by using flow cytometry. The method comprises steps of: preparing a unicell fluid suspension by using an extraction cracking buffer and a dyeing buffer; determining DNA fluorescence intensity of a sample by using a CyFlow Cube type of flow cytometer produced by German partec company and carrying out ploidy analysis; and comparing the abscissa positions of the sample to be measured and the contrast DNA content fluorescence intensity separation peak to identify the ploidy of the sample to be measured. A flow cytometer ploidy identification result is consistent with root tip chromosome counting ploidy. According to the method, ploidy of batch of Gerbera jamesonii macrospore regeneration plant can be screened out rapidly, and efficiency of DH colony construction and heredity atlas drafting is increased substantially, so as to accelerate a breeding process. The invention has advantages of simple sample preparation, rapid detection and stable high detection rate.

The invention discloses a pure plant formaldehyde remover used for decoration. The pure plant formaldehyde remover used for the decoration comprises the following constituents: by weight, 10-15 parts of chlorophytum comosum, 10-15 parts of sansevieria, 10-15 parts of hedera helix, 3-7 parts of aloe, 10-15 parts of century plant, 10-15 parts of gerbera jamesonii and 10-15 parts of dayflower. The pure plant formaldehyde remover used for the decoration is natural in formula and good in formaldehyde removing effect.

An optimized substrate for tissue culture, transplantation and hardening-off of gerbera jamesonii relates to a method for tissue culture of flowers, which is characterized in that the substrate mainlyconsists of saw dust, moor soil and laterite according to the volume proportion of 2:1:1, and the pH value of the substrate is controlled between 6.2 and 6.8. The method has the following advantagesthat: the adding of the saw dust saves cost, and simultaneously improves the ventilation capability and water permeability of the substrate; as a conventional component of a soilless culture medium, the moor soil guarantees various physico-chemical structures of the substrate; and the adding of the laterite not only can adjust the pH value of the substrate, but also can enhance the water-holding and fertilizer-conserving capacity of the substrate.

The invention belongs to the technical field of agriculture and particularly relates to a base fertilizer for gerbera jamesonii planting and a preparation method thereof. The base fertilizer for gerbera jamesonii planting is prepared from, by weight, 20-30 parts of package material, 10-15 parts of chestnut shells, 10-20 parts of biochar based fertilizer, 10-20 parts of edible fungus culture medium by-product, 10-20 parts of broadly beneficial bacteria, 10-15 parts of yellow sand, 5-15 parts of nitrification inhibitor, 5-15 parts of molasses fermentation waste, 5-15 parts of sawdust, 5-15 parts of bamboo vinegar fluid, 3-10 parts of slag, 1-10 parts of bat guano and 1-10 parts of compound fertilizer. The fertilizer is reasonable in composition and contains a variety of elements, trace elements and organic matter, the obtained base fertilizer is homogeneous in nutrition proportion, further has a certain insect repellent ability, can slowly release nutrients and ensures that gerbera jamesonii moderately absorbs the fertilizer in the growth process and grows in balanced mode, grown leaves are large and grown branches and leaves are uniform, ornamental and decorative values are improved, and further the economic benefits are further increased. The preparation method is simple and is easily understood.

The invention belongs to the technical field of agriculture, and particularly relates to a base fertilizer for planting the Gerbera jamesonii and a manufacturing method thereof. The base fertilizer for planting the Gerbera jamesonii consists of the following raw materials in parts by weight: 20-30 parts of wrapping materials, 10-15 parts of peanut hulls, 10-20 parts of charcoal-based fertilizer, 10-20 parts of edible mushroom culture medium by-product, 10-20 parts of broadly beneficial bacteria, 10-15 parts of vermiculite, 5-15 parts of nitrification inhibitor, 5-15 parts of molasses fermentation waste, 5-15 parts of plant ash, 5-15 parts of bamboo vinegar, 3-10 parts of slag, 1-10 parts of goat dung, and 1-10 parts of compound fertilizer. The fertilizer is reasonable in formula, rich in various major elements, trace elements and organic matters. Meanwhile, the obtained base fertilizer is uniform in nutrition-allocated proportion and has a certain insect repellent capability. Nutrients can be slowly released, so that the Gerbera jamesonii can moderately absorb the fertility during the growing process. Therefore, the balanced growth of the Gerbera jamesonii is ensured. The flowers of the Gerbera jamesonii are large and the branches/leaves of the Gerbera jamesonii are uniform. The ornamental and decorative value is improved, so that the economic benefit is further increased. The preparation method is simple and easy to understand.

The invention provides a breeding method of a gerbera jamesonii homozygote plant, belonging to the field of biotechnology. The breeding method comprises the steps of: through flower bud selection, low-temperature treatment and disinfection, picking off ovules and introducing into a same culture medium for synchronously carrying out callus induction, bud differentiation and subculture, carrying out chromosome reduplication on a primarily selected haplobiont, primarily selecting a reduplicated strain for expanding propagation and rooting culture, hybridizing in field strains, and selecting again to obtain an excellent homozygote plant. According to the invention, the callus induction, bud differentiation and subculture of ovules are carried out by using one culture medium, and thus a tissue culture flow is simplified; and by adopting a method of combining indoor indirect evaluation and field direct evaluation, the step of evaluating the ploidy of chromosome is omitted, the difficulty that a genotype is not homozygous due to a chimera existing in an obtaining and reduplication process of the haplobiont is avoided, the gerbera jamesonii homozygote plant can be simply and rapidly bred and propagated, and a selfing life with excellent and stable characters is provided for the breeding of new variety.

The invention relates to a method for rejuvenating degenerated plants and particularly relates to a method for rejuvenating degenerated gerbera jamesonii bolus. The method comprises the following steps: culturing the roots of the degenerated gerbera jamesonii bolus, wherein the roots of the degenerated gerbera jamesonii bolus serve as explants; selecting the lower sections of the roots of the degenerated gerbera jamesonii bolus, wherein the selected lower sections can be dedifferentiated to form callus, and the callus can grow into adventitious buds; inducing the adventitious buds, and performing enrichment culture to obtain rooted seedlings. By adopting the method, the seedlings can grow vigorously, the influence of continuous cropping soil to the growth and quality of the gerbera jamesonii bolus seedlings can be eliminated, the soil utilization efficiency can be improved, the character degenerated gerbera jamesonii bolus can be rejuvenated and reutilized, and money spent in purchasing high-quality gerbera jamesonii bolus seedlings of gerbera jamesonii bolus seedlings breeding companies can be saved.

The invention discloses a preparation method of a decoration pure-plant formaldehyde remover. The formaldehyde remover comprises the following ingredients in parts by weight: 10-15 parts of chlorophytum comosum, 10-15 parts of sansevieria trifasciata, 10-15 parts of hedera helix, 3-7 parts of aloe, 10-15 parts of century plant, 10-15 parts of gerbera jamesonii and 10-15 parts of commelina communis. The preparation method comprises the following specific steps: drying chlorophytum comosum, sansevieria trifasciata, hedera helix, aloe, century plant, gerbera jamesonii and commelina communis; smashing; weighting according to the ratio; uniformly mixing to obtain the decoration pure-plant formaldehyde remover. The formaldehyde remover is natural in formula and good in effect of eliminating formaldehyde.

The invention provides a gerbera jamesonii receptacle induction method and relates to a flower culture method. The gerbera jamesonii receptacle induction method comprises the following steps of: selecting explants, disinfecting the explants, selecting receptacle induction media and selecting culture conditions and modes. Tender gerbera jamesonii buds are vertically cut into four blocks after calyx and small inflorescence peeling and are then inoculated into the induction culture medium for normal light illumination culture. The method is characterized in that in the step of selecting the explants, explants with the bud diameter being 0.5 to 0.8cm are used as selection objects, and in addition, the cold storage pre-treatment on the selected objects for 48 hours at 4 DEG C is added; in the step of selecting receptacle induction media, the selected culture medium has a recipe consisting of 10mg/L 1/2MS+6-BA, 0.5mg/L of NAA, 30g/L of cane sugar and 7g/L of agar, and the pH value is controlled to be about 5.8; and the culture conditions and modes are as follows: 7-day dark pre-culture is firstly carried out, then, the normal light illumination culture is adopted, the light illumination intensity is 1500 to 2000lx, the light illumination time is 14hours/day, and the temperature is 23 to 25 DEG C until annual seedlings are obtained through culture. The induction rate of the receptacle callus and the differentiation rate of adventitious buds are improved.

The invention discloses a method for doubling a haplobiont of gerbera. The method for doubling the haplobiont of the gerbera comprises the following steps: double haplobiont plants are finally determined when tissue-cultured breeding seedlings of the haplobiont of the gerbera go through strong seedlings and multiplication culture, chemical mutagenesis culture, rejuvenation culture, variant plantsscreening, breed conservation of primary plants, propagation, rooting culture, mutagenic strains reselection, identification of stomata guard cells chloroplast counting method, and identification of root tip chromosome counting method. The ploidy doubling cultivation of haplobiont of the gerbera and identification processes are optimized, while mutation rate of the haplobiont is increased, and theaccuracy of ploidy identification is guaranteed. The method for doubling the haplobiont of the gerbera can effectively reduce the formation and interference of chimeras, reduce the damage of chemicaldrugs to the plants to reduce death rate of the haplobiont, and keep variation rate of the haplobiont between 60-80%, and provides the double haplobiont with excellent traits and high stability for breeding of new variety of the gerbera.

The invention discloses organic compound fertilizer for planting gerbera jamesonii. The organic compound fertilizer is prepared from components in parts by weight as follows: 55-70 parts of fermented straw, 20-30 parts of grass carbon, 14-22 parts of sodium alginate, 15-20 parts of peat, 10-20 parts of pig manure, 10-14 parts of potassium fulvate, 8-15 parts of ammonium phosphate, 3-7 parts of phosphogypsum, 4-10 parts of manganese sulfate, 5-10 parts of shell powder, 6-12 parts of corrupted leaves, 4-8 parts of attapulgite, 5-10 parts of wormcast, 1-7 parts of a soil conditioner and 1-6 parts of compound microorganisms. The organic compound fertilizer is high in activity and contains a large quantity of organic and inorganic nutrient substances required for gerbera jamesonii growth, and nutrients of the fertilizer can be balanced; meanwhile, sufficient raw materials are prepared, the preparation is simple, fertilizer formation time is short, water and fertilizer retention capacity of soil is enhanced, and growth of gerbera jamesonii plants is promoted.

This invention discloses the pretreatment liquor for Africa mum. The character: the pretreatment liquor consists thiabendazole, citric acid, 6-benzyl purine, hyposulphite, silver nitrate and qulatong soluted in water in proportion. Using the pretreatment liquor of This invention discloses good for the come out of Africa mum flowers and delayed the withering of flowers.

The invention relates to insecticidal organic environment-friendly fertilizer which is prepared from the following raw materials in parts by weight: 350-450 parts of livestock manure, 250-320 parts ofsawdust, 50-80 parts of plant ash, 30-50 parts of urea, 18-22 parts of monopotassium phosphate, 60-90 parts of tea stalk, 150-180 parts of tea leaves, 80-85 parts of straw, 12-15 parts of distillers'grains, 5-8 parts of common threewingnut root, 2-4 parts of cortex meliae, 3-5 parts of fruit of farges evodia, 5-7 parts of flos gerbera jamesonii, 3-5 parts of European verbena herb, 1-3 parts of castor oil plant, 1-3 parts of Chinese honey locust and 2-3 parts of herba garland chrysanthemi. The environment-friendly fertilizer provided by the invention is environmentally friendly and non-toxic,realizes a good insecticidal effect on the overground plant body part and the underground root system and the fruit part as well as an inhibitory effect on bacteria in the air so as to purity the air; moreover, with stable fertilizer efficiency and long duration, the insecticidal organic environment-friendly fertilizer provided by the invention can provide necessary nutrients for the plant growthso as to guarantee rapid and stable growth of the plant.

The invention provides a tissue culture method of gerbera jamesonii, and belongs to the technical field of plant tissue culture. The method comprises the following steps of 1) carrying out callus culture and subculture multiplication culture on sterile gerbera jamesonii leaves with petioles to obtain calluses; and 2) carrying out differentiation culture and subculture on the calluses obtained in the step 1) to obtain gerbera jamesonii clustered seedlings. By adopting the tissue culture method provided by the invention, the callus induction rate is high, the propagation speed is high, the callus differentiation efficiency is high, the number of cluster buds is large, the bud growth speed is high, the propagation coefficient of gerbera jamesonii can be greatly increased, the propagation speed is increased, the seedling period is shortened, and large-scale cultivation and cut flower production of the gerbera jamesonii are greatly facilitated.

The invention discloses an in-vitro hybridization method for gerbera jamesonii and belongs to the technical field of gerbera jamesonii crossbreeding. Male parent pollens are collected, a flower cutting in-vitro vase pollination of gerbera jamesonii is carried out, and hybrid seeds are obtained. The method provides a new way for breeding, overcomes adverse factors such as high temperature, high humidity, diseases and the like which are easy to occur in a hybridization process of gerbera jamesonii, and remarkably improves a success rate of gerbera jamesonii hybridization.

The invention discloses a rooting and seedling strengthening method for tissue culture seedlings of gerbera jamesonii. The rooting and seedling strengthening method comprises the following steps: cutting clustered tissue culture seedlings of gerbera jamesonii into single buds, performing lighting culture on a seedling strengthening rooting culture medium, inducing rooting, strengthening the seedlings in covered bottles for 1-3 months in a greenhouse or a culture room after rooting, gradually opening the bottles to strength the seedlings, and performing heel-in in a cultivation medium. By adopting the seedling strengthening rooting culture medium, sufficient nutrition can be provided, the growth speed of the tissue culture seedlings of gerbera jamesonii can be appropriately reduced, the maintenance and management time of the tissue culture seedlings of gerbera jamesonii on a seedbed can be shortened, production of the tissue culture seedlings of gerbera jamesonii is not interrupted in hot summer and cold winter, the labor and the water and power cost of seedbed management are greatly reduced, the method is simple to operate and convenient to manage, the tissue culture seedlings of gerbera jamesoni, which is cultured by using the method, are good in root system development, the survival rate after heel-in is greater than 99%, and the seedlings can all survive after being transplanted fixedly.

The invention belongs to the technical field of fertilizers, and discloses a special fertilizer for Gerbera jamesonii Bolus. The special fertilizer is prepared from the following raw materials in parts by weight: potassium dihydrogen phosphate 20-60 parts, potassium sulfate 10-30 parts, ammonium dihydrogen phosphate 5-20 parts, zinc sulfate 1-10 parts, a vermiculite extracting solution 10-50 parts, Fructus Toosendan extract 10-30 parts, tea seed extract 5-10 parts and boric acid 1-10 parts. The invention further discloses a preparation method of the special fertilizer for Gerbera jamesonii Bolus. The preparation method comprises the following steps: after successively mixing the raw materials evenly, adding the boric acid to regulate pH of a solution to be 5.5-7.0; and then pelletizing and drying to obtain the final product. The fertilizer is specially prepared according to growth characteristics of Gerbera jamesonii Bolus, and is comprehensive in nutrition; the utilization rate of the fertilizer for the Gerbera jamesonii Bolus can be increased effectively; and absorption, adult plant blossom improving, sterilizing and insect expelling and healthful growth of the Gerbera jamesonii Bolus are promoted.

The invention belongs to the technical field of plant planting, and in particular discloses a gerbera jamesonii planting method. The gerbera jamesonii planting method comprises the following steps of(1) selecting seeds; (2) pretreatment: placing the seeds treated by the step (1) into a treating fluid for soaking, first soaking the seeds at 1-4 degrees centigrade for 0.8-1.2 h, then soaking the seeds at 10-15 degrees centigrade for 1-1.5 h, and finally soaking the seeds at 23-28 degrees centigrade for 1.5-2 h; (3) cultivating the pretreated seeds so that the seeds grow into seedlings; (4) field planting: selecting a planting land, digging holes in the planting land, controlling the depth of the holes to be 12-18 cm and the area of the holes to be 200-360 cm<2>, transplanting the gerbera jamesonii seedlings, so that roots of the gerbera jamesonii seedlings stretch into the holes, and fertilizing mixed matrixes into the holes; and (5) daily management. When the gerbera jamesonii is planted by using the cultivation method, sufficient and continuous fertility can be provided for gerbera jamesonii and the normal growth of gerbera jamesonii can be guaranteed.

The invention discloses a rapid propagation method of potted gerbera jamesonii bolus tissue culture. The method comprises the following steps: shearing a gerbera jamesonii bolus receptacle into small blocks, sterilizing and placing in an MS culture medium containing 0.5mg/L of 6-BA and 0.1mg/L of NAA to induce, producing adventitious bud at an explant edge to acquire a sterile young plant, continuously placing blades of the sterile young plant in the culture medium as the explants, subculturing in the culture medium to obtain a robust plant, transferring the obtained robust plant into an MS rooting culture medium containing 0.3g/L of IBA to culture for 25-30 days to obtain a complete rooted plant, picking out an acquired rooted seedling from a culture bottle, cleaning and transplanting into a 72-cavity plate filled with matrix, gradually lowering relative air humidity to perform domestication and cultivation for 6 weeks, namely acquiring a transplanting stock. The young seedling obtained by use of the method disclosed by the invention is robust and high in survival rate, a great deal of high quality seedlings of the potted gerbera jamesonii bolus can be provided within short term, and the method has important effect on the scale promotion of the potted gerbera jamesonii bolus.

The invention discloses a cross-breeding method of gerbera jamesonii in a sunlight greenhouse tent. By adopting frame-type substrate culture, fertilizers are applied quantitatively and watering operation is performed according to the plant growth condition.Poorly-growing plants can be separately managed. The cross-breeding method of gerbera jamesonii in the sunlight greenhouse tent has following beneficial effects: diseases are conveniently controlled; when diseases occur in one of frame, plants can be quarantined in order to separate control; diseases do not break out in a large scale; soil-borne diseases such as wilt diseases and root-rot diseases are effectively controlled so that the seed setting number is increased; by adoption of frame-type substrate culture, plants grow robustly in order to favorably raise seed setting number; and according to the breeding need, relative positions of male parents and female parents can be randomly placed so that great practicability is obtained.

The invention discloses a method for improving quality of a cut flower of gerbera by utilizing CO2. A timed and quantitative enriching technology of the CO2 is adopted, a nitrogenous fertilizer using measure is combined, and the interaction of the carbon-nitrogen metabolism is utilized, so that the flowering time of the gerbera is advanced, the diameter of the flower is enlarged, the number of the flower in a single plant is increased, the color of the flower is deepened, the consistency of the flower is good, the vase life of the cut flower is prolonged, the method is suitable for the gerbera planted by a conventional method in a green house, gerbera in different cultivars and different growth periods, the production cost is reduced, and besides, the generation of the greenhouse effect is avoided.

The invention provides an ecological and environment-friendly herbicide based on sagittaria graminea. The sagittaria graminea is aqueous extract of sagittaria graminea. Gerbera jamesonii is taken as arecipient plant for researching the allelopathy of the aqueous extract of the sagittaria graminea for the recipient plant, it is discovered that different concentrations of the aqueous extract of thesagittaria graminea have different allelopathic effects on germination of Gerbera jamesonii seeds, the aqueous extract with mass concentrations of 0.05 g.mL<-1> and 0.025 g.mL<-1> promotes germination of the Gerbera jamesonii seeds, while the aqueous extract with mass concentrations of 0.1 g.mL<-1> 0.075 g.mL<-1> inhibits germination of the Gerbera jamesonii seeds. By adopting the aqueous extractof sagittaria graminea for eliminating or inhibiting the alien species Gerbera jamesonii, on one hand, invasion of the alien species is inhibited, on the other hand, weed control is realized by the plant preparation, and environmental pollution caused by use of a chemical herbicide and adverse impact caused by herbicide residues are avoided.

The invention discloses a planting soil conditioner for gerbera jamesonii bolus. The planting soil conditioner is prepared from components in parts by weight as follows: 35-70 parts of fermented chicken manure, 15-22 parts of calcium silicate, 10-20 parts of coal gangue powder, 8-12 parts of tetramethrin, 12-16 parts of desulfurization gypsum, 8-13 parts of rice bran, 5-9 parts of magnesium nitrate, 7-10 parts of ammonium din phosphate, 6-12 parts of potassium chloride, 7-11 parts of bamboo charcoal powder, 4-8 parts of sodium acrylate, 3-8 parts of fulvic acid, 5-10 parts of amino acid, 1-6 parts of microelements, 2-6 parts of alkaloid, 1-5 parts of a water-retaining agent and 1-4 parts of a rooting agent. The prepared soil conditioner contains various nutrients required for growth of the gerbera jamesonii bolus, the original softness and vitality of soil can be thoroughly recovered, planting of high-quality gerbera jamesonii bolus and growth of the gerbera jamesonii bolus are promoted, and the yield and ornamental value are increased.