Tissue culture Quick detoxification propagating method for OT type hybrid lily

Abstract

The invention discloses a virus-free tissue culture and rapid propagation method for OT type hybrid lily, and belongs to the technical field of plant tissue culture and rapid propagation. The method comprises: preparation of a culture medium, taking lily provenance bulb shoot tips as explants, and rapidly obtaining a large amount of virus-free tissue-cultured seedlings through steps such as induction, proliferation, bulb expansion culture, cold storage, transplanting, and virus detection. The present invention adjusts the formula of the culture medium, and adopts technologies such as secondary stem tip heat treatment for detoxification and induction culture, so as to directly induce bulb buds and / or rootless tissue cultured seedlings without going through the callus growth stage, thereby preventing the growth of seedlings. Sexual variation, shortening the induction culture period, has the characteristics of complete detoxification, high multiplication coefficient, low production cost, strong practicability, etc., and can be popularized and applied in the detoxification tissue culture production of lilies and other plants.

Description

technical field

[0001] The invention relates to the technical field of plant tissue culture and rapid propagation, in particular to a method for virus-free tissue culture and rapid propagation of OT hybrid lilies. Background technique

[0002] OT-type hybrid lily (Oriental×Trumpet Hybrid) is a hybrid series of Oriental Lily and Trumpet Lily / Aurelianos hybrid line. It is a perennial ornamental plant of Liliaceae, mainly used for cut flower cultivation. Its plant stem strength, disease resistance, heat resistance, salt and alkali resistance and other traits are obviously better than oriental lily, so it has a good market development prospect. In recent years, the production of cut lily flowers in my country has increased by more than 20% annually, but lily bulbs have always been dependent on imports from abroad. However, viral disease infection has caused serious degradation of lily species, which has brought huge economic losses to lily bulb producers around the world. There...

Examples

Embodiment 1

[0033] Embodiment 1: (OT type hybrid line lily virus-free tissue culture rapid propagation method 1)

[0034] The specific method is as follows:

[0035] (1) Preparation and standby of the culture medium: the components including the basic medium and the culture medium at each stage of tissue culture and the weight of each component in each liter of culture medium are formulated as follows.

[0036] (2) Selection and sterilization of explants: In early March, the dormant and broken bulbs were taken, treated with heat and humidity at 50°C for 70 minutes, soaked in 75% alcohol for 0.5 minutes, rinsed with sterile water for 3 to 5 times, and then Sterilize with 0.1% mercuric chloride solution for 15 minutes, rinse with sterile water for 3 to 5 times, sterilize with 10% sodium hypochlorite aqueous solution for 15 minutes, and finally rinse with sterile water for 3 to 5 times, as explants for detoxification tissue culture .

[0037] (3) Induction culture: Take a 0.2-0.3mm shoot t...

Embodiment 2

[0043] Embodiment 2: (OT type hybrid line lily virus-free tissue culture rapid propagation method 2)

[0044] (1) Preparation and standby of the culture medium: the components including the basic medium and the culture medium at each stage of tissue culture and the weight of each component in each liter of culture medium are formulated as follows.

[0045] (2) Selection and sterilization of explants: In mid-March, take bulbs that have been broken from dormancy, heat and treat them at 50°C for 90 minutes, soak them in 75% alcohol for 1.0 minutes, wash them with sterile water for 3 to 5 times, and then Sterilize with 0.1% mercuric chloride solution for 20 minutes, rinse with sterile water for 3 to 5 times, sterilize with 10% sodium hypochlorite aqueous solution for 13 minutes, and finally rinse with sterile water for 3 to 5 times, as explants for detoxification tissue culture .

[0046] (3) Induction culture: Take a 0.2-0.3mm shoot tip under the objective lens and inoculate it ...

Embodiment 3

[0052] Embodiment 3: (OT type hybrid line lily virus-free tissue culture rapid propagation method 3)

[0053] (1) Preparation and standby of the culture medium: the components including the basic medium and the culture medium at each stage of tissue culture and the weight of each component in each liter of culture medium are formulated as follows.

[0054] (2) Selection and sterilization of explants: In the first ten days of March, the dormant and broken bulbs were taken, treated with heat and humidity at 50°C for 80 minutes, soaked in 75% alcohol for 0.75 minutes, rinsed with sterile water 3 to 5 times, and then Sterilize with 0.1% mercuric chloride solution for 18 minutes, rinse with sterile water for 3 to 5 times, sterilize with 10% sodium hypochlorite aqueous solution for 14 minutes, and finally rinse with sterile water for 3 to 5 times, as explants for detoxification tissue culture .

[0055] (3) Induction culture: Take a 0.2-0.3mm shoot tip under the objective lens and ...

technical field

[0001] The invention relates to the technical field of plant tissue culture and rapid propagation, in particular to a method for virus-free tissue culture and rapid propagation of OT hybrid lilies. Background technique

[0002] OT-type hybrid lily (Oriental×Trumpet Hybrid) is a hybrid series of Oriental Lily and Trumpet Lily / Aurelianos hybrid line. It is a perennial ornamental plant of Liliaceae, mainly used for cut flower cultivation. Its plant stem strength, disease resistance, heat resistance, salt and alkali resistance and other traits are obviously better than oriental lily, so it has a good market development prospect. In recent years, the production of cut lily flowers in my country has increased by more than 20% annually, but lily bulbs have always been dependent on imports from abroad. However, viral disease infection has caused serious degradation of lily species, which has brought huge economic losses to lily bulb producers around the world. There...

Examples

Embodiment 1

[0033] Embodiment 1: (OT type hybrid line lily virus-free tissue culture rapid propagation method 1)

[0034] The specific method is as follows:

[0035] (1) Preparation and standby of the culture medium: the components including the basic medium and the culture medium at each stage of tissue culture and the weight of each component in each liter of culture medium are formulated as follows.

[0036] (2) Selection and sterilization of explants: In early March, the dormant and broken bulbs were taken, treated with heat and humidity at 50°C for 70 minutes, soaked in 75% alcohol for 0.5 minutes, rinsed with sterile water for 3 to 5 times, and then Sterilize with 0.1% mercuric chloride solution for 15 minutes, rinse with sterile water for 3 to 5 times, sterilize with 10% sodium hypochlorite aqueous solution for 15 minutes, and finally rinse with sterile water for 3 to 5 times, as explants for detoxification tissue culture .

[0037] (3) Induction culture: Take a 0.2-0.3mm shoot t...

Embodiment 2

[0043] Embodiment 2: (OT type hybrid line lily virus-free tissue culture rapid propagation method 2)

[0044] (1) Preparation and standby of the culture medium: the components including the basic medium and the culture medium at each stage of tissue culture and the weight of each component in each liter of culture medium are formulated as follows.

[0045] (2) Selection and sterilization of explants: In mid-March, take bulbs that have been broken from dormancy, heat and treat them at 50°C for 90 minutes, soak them in 75% alcohol for 1.0 minutes, wash them with sterile water for 3 to 5 times, and then Sterilize with 0.1% mercuric chloride solution for 20 minutes, rinse with sterile water for 3 to 5 times, sterilize with 10% sodium hypochlorite aqueous solution for 13 minutes, and finally rinse with sterile water for 3 to 5 times, as explants for detoxification tissue culture .

[0046] (3) Induction culture: Take a 0.2-0.3mm shoot tip under the objective lens and inoculate it ...

Embodiment 3

[0052] Embodiment 3: (OT type hybrid line lily virus-free tissue culture rapid propagation method 3)

[0053] (1) Preparation and standby of the culture medium: the components including the basic medium and the culture medium at each stage of tissue culture and the weight of each component in each liter of culture medium are formulated as follows.

[0054] (2) Selection and sterilization of explants: In the first ten days of March, the dormant and broken bulbs were taken, treated with heat and humidity at 50°C for 80 minutes, soaked in 75% alcohol for 0.75 minutes, rinsed with sterile water 3 to 5 times, and then Sterilize with 0.1% mercuric chloride solution for 18 minutes, rinse with sterile water for 3 to 5 times, sterilize with 10% sodium hypochlorite aqueous solution for 14 minutes, and finally rinse with sterile water for 3 to 5 times, as explants for detoxification tissue culture .

[0055] (3) Induction culture: Take a 0.2-0.3mm shoot tip under the objective lens and ...