Method for detoxifying radix tetrastigme through vitrification ultralow temperature therapy

A technology of ultra-low temperature vitrification and clover, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of low plant tissue survival rate and low virus clearance rate, and improve the survival rate of recovery culture, damage reduction effect

Active Publication Date: 2021-08-24
SHANGRAO NORMAL UNIV
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Problems solved by technology

But at present, this method still has the problems of low surv

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  • Method for detoxifying radix tetrastigme through vitrification ultralow temperature therapy

Examples

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Effect test

Embodiment 1

[0032] The detoxification steps of trefoil vitrification ultra-low temperature therapy are as follows:

[0033] (1) Sterile seedling cultivation: take the bud stem section with TMV cloverleaf after detection, rinse it with water, soak it with 0.1% mercuric chloride solution for 30min under aseptic conditions, then transfer it to the sodium hypochlorite solution for disinfection and disinfection Afterwards, rinse with sterile water, use sterile paper to blot the moisture on the surface of the bud stem section of A. trifoliate, and transfer it to MS medium containing 6-BA 1.0mg / L and NAA 0.5mg / L. It is 2000Lux, and the light time is 12h·d -1 , cultured at a temperature of 25±1°C for 5 days to obtain sterile seedlings of Clover.

[0034] (2) Pre-cultivation: the aseptic seedlings of A. trilobata cultivated in step (1) are 2000Lux in light intensity, and the light time is 12h·d -1 , and the temperature was 40±2°C to continue culturing for 7 days, and the medium was MS medium con...

Embodiment 2

[0039] The detoxification steps of trefoil vitrification ultra-low temperature therapy are as follows:

[0040](1) Sterile seedling cultivation: take the bud stem section with TMV cloverleaf after detection, rinse it with water, soak it with 0.1% mercuric chloride solution for 30min under aseptic conditions, then transfer it to the sodium hypochlorite solution for disinfection and disinfection Afterwards, rinse with sterile water, use sterile paper to blot the moisture on the surface of the bud stem section of A. trifoliate, and transfer it to MS medium containing 6-BA 1.0mg / L and NAA 0.5mg / L. It is 2000Lux, and the light time is 12h·d -1 , cultured at a temperature of 25±1°C for 5 days to obtain sterile seedlings of Clover.

[0041] (2) Pre-cultivation: the aseptic seedlings of the three-leaf greens cultivated in step (1) are 1800Lux in light intensity, and the light time is 13h·d -1 , and the temperature was 40±2°C for 8 days, and the culture medium was MS medium containin...

Embodiment 3

[0046] The detoxification steps of trefoil vitrification ultra-low temperature therapy are as follows:

[0047] (1) Sterile seedling cultivation: take the bud stem section with TMV cloverleaf after detection, rinse it with water, soak it with 0.1% mercuric chloride solution for 30min under aseptic conditions, then transfer it to the sodium hypochlorite solution for disinfection and disinfection Afterwards, rinse with sterile water, use sterile paper to blot the moisture on the surface of the bud stem section of A. trifoliate, and transfer it to MS medium containing 6-BA 1.0mg / L and NAA 0.5mg / L. It is 2000Lux, and the light time is 12h·d -1 , cultured at a temperature of 25±1°C for 5 days to obtain sterile seedlings of Clover.

[0048] (2) Pre-cultivation: the aseptic seedlings of A. trilobata cultivated in step (1) are 2000Lux in light intensity, and the light time is 12h·d -1 , and the temperature was 40±2°C to continue culturing for 6 days, and the medium was MS medium con...

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Abstract

The invention discloses a method for detoxifying radix tetrastigme through vitrification ultralow temperature therapy, and belongs to the technical field of plant detoxification. The method comprises the following specific steps that (1), aseptic seedlings of radix tetrastigme is pre-cultured; (2), stem tips of the aseptic seedlings of the radix tetrastigme pre-cultured in the step (1) are cut, and differentiation culture is carried out to obtain seedlings of the radix tetrastigme; (3), stem tips of the radix tetrastigme seedlings obtained in the step (2) are cut, the stem tips are loaded by using a PVS2 solution with the concentration of 50%-60%, the stem tips are dehydrated by using a PVS2 solution containing nano calcium carbonate, and a container filled with the PVS2 solution and the stem tips are frozen at ultralow temperature; and (4), the stem tips frozen at the ultra-low temperature in the step (3) is thawed, and then recovery culture is carried out to obtain the detoxified seedlings of the radix tetrastigme. The stem tips of the radix tetrastigme treated by the detoxification method of the vitrification ultralow temperature therapy are high in survival rate, and the detoxification rate of survived seedlings is further high.

Description

technical field [0001] The invention relates to the technical field of detoxification of plants, in particular to a method for detoxification of trefoil vitrification ultra-low temperature therapy. Background technique [0002] Raidxe Tetrastigmae, also known as golden thread gourd, is a herbaceous vine of Tetrastigma hemsleyanum Diels et Gilg in the grape family. The whole plant can be used as medicine, and the medicinal effect of the underground tuber is the best. Its main components are flavonoids, phenolic acids, triterpenoids, steroids and stilbenes. Pharmacological activity studies have shown that Clover has various biological activities such as anti-tumor, anti-oxidation, immune regulation, anti-virus, anti-inflammatory, analgesic, antipyretic, and liver protection. It is clinically used to treat febrile convulsions, abdominal pain, pneumonia, asthma, hepatitis and other diseases, and it is widely used among the people. [0003] In the planting process of clover, v...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 尹明华王文霞王艳敏文心雨谢莲熊艺馨熊梓伊
Owner SHANGRAO NORMAL UNIV
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