Method for rapidly removing three viruses of lily

A technology for removing and removing lily, applied in the directions of virus/phage, biochemical equipment and methods, seed and rhizome treatment, etc., can solve the problem of low sensitivity, and achieve the effect of improving the detoxification rate, easy cutting and survival rate.

Inactive Publication Date: 2008-05-07
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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Problems solved by technology

However, the following problems still exist in the existing detection methods: stem (root) tip culture (stem or shoot tip culture) is an aseptic culture in which the apex part of the stem (root) or the stem (root) tip meristem part is cut.
At the same time, related to the virus detection technology and method, indicator plants and ELISA were used for detection in the early stage, and the sensitivity was low, and the virus was not completely removed in many cases.

Method used

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  • Method for rapidly removing three viruses of lily
  • Method for rapidly removing three viruses of lily

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Embodiment Construction

[0016] 1) Detoxification method

[0017] Take bulbs that have sprouted 3 to 6 cm after low-temperature treatment, cut them off from the stem nodes, and remove the outer scales. Rinse with tap water for 1 hour, soak in distilled water for 10 minutes, then disinfect with 0.1% mercuric chloride for 10 minutes (add a few drops of Tween 80), and finally rinse with sterile water for 3 to 5 times. Under sterile conditions with a stereomicroscope (Olympus, SZX10) with a micrometer, cut shoot apical meristems with 1-2 leaf primordia and different sizes, and quickly inoculate them on medium supplemented with different concentrations of ribavirin , for shoot tip culture. The medium composition is: MS+IBA1.0mg / L+NAA0.1mg / L, sucrose 3%, agar 6g / L. The culture conditions are 2000-3000lx, the light time is 14h / d, and the culture temperature is 20-25°C.

[0018] According to the uniform test design, the shoot tip size was set to 4 levels 0.2-0.4mm, 0.5-0.7mm, 0.8-1.0mm, 1.0-1.2mm; the riba...

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Abstract

The invention relates to a method for rapid removing three sorts of virus in lily, belonging to biologic technical field, which is characterized in that: the meristematic tissue of stem tip is inoculated in the culture medium with ribavirin; wherein, the size of the stem tip is 0.5 to 0.8mm, and the concentration of the ribavirin is 4 mg/L to 6 mg/L. The invention has the advantages of high detoxication rate, high survival rate and rapid regeneration speed.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for rapidly removing three viruses from lilies. Background technique [0002] The three lily viruses LSV, CMV and LMoV are the main reasons for the yield reduction of lily commercial crops. Existing main methods for removing the above-mentioned three kinds of viruses are as follows: 1) shoot apex meristem method: the shoot apex meristematic zone is the zone with the least virus content. The shoot apical meristem is limited to the apical conical region, and its length does not exceed 0.1mm in sterile culture. However, such a small shoot tip is actually difficult to obtain, the survival rate is very low, and the time for cultivating into seedlings is also very long. Therefore, in actual cultivation, growth cones with leaf primordia are often used for cultivation, usually with shoot apical meristems of 0.2 to 0.4 mm (millimeters), but the probability of poisoning increases. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A01C1/00
Inventor 明军徐榕雪刘春穆鼎汤庚国王晓武刘博
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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