Method of removing virus for Jiangxi Qianshan red-bud taro through vitrification cryopreservation

Abstract

The invention provides a method of removing virus for Jiangxi Qianshan red-bud taro through vitrification cryopreservation. The method comprises: (1) cutting shoot tips with a length being about 1 mm of single buds of the red-bud taro under a microscope; (2) pre-culturing the shoot tips on a solid medium of MS + KT 2 mg / L + NAA 0.5 mg / L +0.75 M sucrose for three days; (3) after the shoot tips are pre-cultured, using a 60 % PVS2 to dehydrate for 60 minutes under 25 DEG C, then using a 100 % PVS2 to dehydrate for 90 minutes under 0 DEG C, replacing with a new PVS2, and then putting into liquid nitrogen; (4) after preservation, defrosting the shoot tips in a 37 DEG C water bath, washing with a liquid medium of MS + KT 2 mg / L + NAA 0.5 mg / L +1.2 M sucrose for 3 times, 10 minutes for each time, culturing the washed shoot tips in dark for 5 days under 25 DEG C, and then culturing under a normal photoperiod with an illumination time being 14 hours per day, a light intensity being about 1500-2000lx, and a culturing temperature being 25 DEG C, wherein the shoot tips can grow into normal buds after about 15 days; and (5) culturing the germinated buds in a rooting medium of 1 / 2 MS + NAA 0.1-0.5 mg / L, thereby obtaining the virus-free seedlings of the red-bud taro. Compared with prior art, the tube-test plantlets of the red-bud taro are high in virus removing rate, the shoot tips can be taken in a large amount and the operation is simple and convenient.

Description

Technical field: [0001] The invention relates to a method for breeding plant seedlings, in particular to a method for detoxification of Jiangxi Yanshan red bud taro by ultra-low temperature preservation through vitrification. Background technique: [0002] Red bud taro is a traditional superior product in Qianshan County, Jiangxi Province, with a long history of planting. In the past ten years, this advantageous industry has developed rapidly and has become the leading industry for farmers in Yanshan County to increase their income and become rich. Qianshan County has also become the largest red bud taro production base in Jiangxi Province. In March 2007, it was approved by China Green Food Committee and The Ministry of Agriculture named it "National Green Food Standardized Production Base County of Red Bud Taro". However, due to the long-term asexual reproduction in production, the virus is increasingly accumulated, the species is degraded, and the yield and quality have d...

AI Technical Summary

Problems solved by technology

However, due to the long-term asexual reproduction in production, the virus has accumulated more and more, the species has degenerated, and the yield and quality have declined year after year, causing great losses to the production of red bud taro.