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Blueberry tissue culture seedling detoxification method and culture method

A culture method and tissue culture seedling technology are applied to the detoxification method and culture field of blueberry tissue culture seedlings to achieve the effects of developed root system, high fruit yield and high survival rate

Active Publication Date: 2017-11-17
丹东天赐花卉有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is no relevant report on the virus-free technology of blueberry seedlings. How to obtain a large number of virus-free blueberry seedlings is the key to the promotion and industrialization of blueberries.

Method used

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  • Blueberry tissue culture seedling detoxification method and culture method

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Comparison scheme
Effect test

Embodiment 1

[0030] The present embodiment provides a kind of culture method of blueberry tissue culture seedling, and it comprises the steps:

[0031] (1) Heat treatment: heat-treat blueberry tissue culture seedlings at 40°C for 3 days;

[0032] (2) Chemical agent treatment: cut out the shoot tips of the tissue cultured seedlings treated in step (1), place the shoot tips in a medium containing ribavirin, and continue to cultivate them for 50 days at 22°C, wherein the The concentration of ribavirin is 0.3mg / L;

[0033] (3) subculture: the stem tip after step (2) is processed in WPM+ZT3mg / L+IBA0.05mg / L medium, after subculture 3 times, new blueberry tissue culture seedling is obtained;

[0034] (4) Rooting culture: the new blueberry tissue culture seedlings gained in step (3) can be hardened and transplanted after being inoculated in WPM+IBA0.05mg / L medium for 15 days.

Embodiment 2

[0036] The present embodiment provides a kind of culture method of blueberry tissue culture seedling, and it comprises the steps:

[0037] (1) heat treatment: blueberry tissue culture seedlings were heat treated at 45°C for 3 days;

[0038] (2) Chemical agent treatment: cut out the shoot tips of the tissue cultured seedlings treated in step (1), place the shoot tips in a medium containing ribavirin, and continue culturing for 45 days at 25°C, wherein the The concentration of ribavirin is 0.25mg / L;

[0039] (3) subculture: the stem tip after step (2) is processed in WPM+ZT3mg / L+IBA0.05mg / L medium, after subculture 3 times, new blueberry tissue culture seedling is obtained;

[0040] (4) Rooting culture: the new blueberry tissue culture seedlings gained in step (3) can be hardened and transplanted after being inoculated in WPM+IBA0.05mg / L medium for 20 days.

Embodiment 3

[0042] The present embodiment provides a kind of culture method of blueberry tissue culture seedling, and it comprises the steps:

[0043] (1) Heat treatment: heat-treat blueberry tissue culture seedlings at 35°C for 3.5 days;

[0044] (2) Chemical agent treatment: cut out the shoot tips of the tissue cultured seedlings treated in step (1), place the shoot tips in a medium containing ribavirin, and continue to cultivate them for 53 days at 21°C, wherein the The concentration of ribavirin is 0.25mg / L;

[0045] (3) subculture: the stem tip after step (2) is processed in WPM+ZT3mg / L+IBA0.05mg / L medium, after subculture 3 times, new blueberry tissue culture seedling is obtained;

[0046] (4) Rooting culture: the new blueberry tissue culture seedlings gained in step (3) can be hardened and transplanted after being inoculated in WPM+IBA0.05mg / L medium for 25 days.

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Abstract

The invention belongs to the technical field of plant tissue culture, and particularly relates to a blueberry tissue culture seedling detoxification method and culture method. The detoxification method comprises the following steps: (1) heat treatment: treating a blueberry tissue culture seedling at 35-50 DEG C for 3-3.5 days; and (2) chemical agent treatment: cutting off a stem apex of the tissue culture seedling treated by the step (1), putting the stem apex in a virazole-containing culture medium, and continuing culture at 21-25 DEG C for 45-55 days, wherein the concentration of the virazole is 0.2-0.3 mg / L. On such basis, the invention also provides a blueberry tissue culture seedling culture method containing the detoxification method. According to the method, the two-stage detoxification treatment is performed to greatly enhance the detoxification rate of the blueberry tissue culture seedling, and the survival rate of the tissue culture seedling is basically not influenced. The detoxified blueberry seedling can not mutate after being transplanted to the field. Therefore, the methods provided by the invention have broad market prospects and important application and popularization values.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a virus-free method and a culture method for blueberry tissue culture seedlings. Background technique [0002] Blueberry fruit has a thin skin, sweet and sour flavor, and has unique health care functions. Blueberry fruit contains more anthocyanins, flavonoids and other physiologically active ingredients, which can effectively prevent the occurrence of brain nerve aging, diabetes and other diseases, enhance heart function, and have excellent anti-cancer effects. It is a fruit with good development prospects in the 21st century. One of the varieties of fruit trees. Therefore, it is of great value and significance to study its non-toxic and efficient reproduction. [0003] At present, the production of blueberries grown in my country usually adopts tissue culture and cutting propagation methods. The application of tissue culture method has a fast propagation speed, w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 潘利军蔡明关文君潘芊芊程堂仁王佳
Owner 丹东天赐花卉有限公司
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