Method for cultivating oncidium

A cultivation method and technology of oncidium group, applied in the field of agricultural biology, can solve the problems of weak stress resistance, weak reproductive ability, and difficulty in rooting of oncidium, and achieve the effects of improving stress resistance and reducing transplanting disease rate.

Inactive Publication Date: 2014-12-24
ZHEJIANG XIAOSHAN COTTON & FLAX RES INST +1
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of oncidium production, such as difficulty in rooting, weak reproductive ability, slow growth rate, long growth cycle, serious diseases in the transplanting stage of training seedlings, low survival rate in bottles, and weak stress resistance, the present invention proposes a The cultivation method of Xinlan, the cultivation method of the present invention has reduced oncidium out of bottle seedling transplanting disease rate, improves stress resistance

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0029] (1) Cultivation of oncidium "Nanxi" tissue culture seedlings.

[0030] Tissue-cultured seedlings of Oncidium "Nanxi" strain were used as materials, and the medium was MS medium, and the number of seedlings per bottle was 6. The size of the seedlings is 3-4cm high, the number of roots is 2-3, and the root length is about 1-2cm. Placed in a tissue culture room at 22 degrees Celsius, cultured for 16 hours in the light and 8 hours in the dark, and set aside.

[0031] (2) Cultivation of Pyromorpha indica

[0032] Pyromorpha indica marginal mycelium preserved in PDA medium is punched with a hole puncher with a diameter of 5mm, the mycelia block is inoculated in a petri dish containing PDA medium for cultivation, and then the petri dish is placed in a constant temperature incubator Among them, culture condition is: 24h darkness, 25 degrees Celsius, cultivate 20 days, choose the fresh, vigorous mycelia of petri dish edge as the inoculation bacterium block of Piriformis indica...

Embodiment 2

[0039] (1) Cultivation of oncidium "lemon" tissue culture seedlings.

[0040] Tissue-cultured seedlings of Oncidium "lemon" strain were used as materials, and the medium was MS medium, and the number of seedlings per bottle was 8. The size of the seedlings is 4-5cm high, the number of roots is 2-3, and the root length is about 1-2cm. The light is cultivated for 16 hours at 25 degrees Celsius; the dark is cultivated for 8 hours at 20 degrees Celsius for standby.

[0041] (2) Cultivation of Pyromorpha indica

[0042] Pyromorpha indica marginal mycelium preserved in PDA medium is punched with a hole puncher with a diameter of 5mm, the mycelia block is inoculated in a petri dish containing PDA medium for cultivation, and then the petri dish is placed in a constant temperature incubator Among them, culture condition is: 24h darkness, 20 degrees Celsius, cultivate 20 days, choose the fresh, vigorous mycelia of petri dish edge as indica piriformis inoculation bacterium block.

[00...

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Abstract

The invention relates to the field of agricultural biotechnology and provides a method for cultivating oncidium, aiming at solving the problems such as rooting difficulty, weak reproductive capacity, low growth speed, long growth period, severe diseases in seedling hardening and transplanting stages, low bottle-out survival rate and weak stress resistance existing during oncidium production. The method for cultivating oncidium comprises the following steps: (1) culturing oncidium tissue culture seedlings by an MS culture medium; (2) culturing Piriformospora indica fungi by a solid PDA culture medium; (3) co-culturing the oncidium and the Piriformospora indica fungi; and (4) performing bottle-out cultivation on the oncidium tissue culture seedlings. According to the cultivation method disclosed by the invention, the disease rate during oncidium bottle-out seedling transplanting is reduced, and the stress resistance is improved.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a method for reducing the transplanting disease of Oncidium vase seedlings and improving stress resistance. Background technique [0002] Oncidium production has serious problems such as difficulty in rooting, weak reproductive ability, slow growth rate, long growth cycle, serious diseases in the seedling transplanting stage, low survival rate of vases, and weak stress resistance. Seeking beneficial fungal resources that can co-exist with orchid flowers is an effective way to solve this problem. [0003] P. indica can colonize the roots of a variety of crops, and its host range is wide, such as Arabidopsis, tobacco, barley, wheat, corn, etc. The hyphae of Pyriformis indica can colonize the surface of plant roots, root epidermal cells and intercellular spaces, forming typical chlamydospores of pyriform spores, which can colonize and survive in crop roots for a long time....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘晓静田丹青潘晓韵李明江葛亚英郁永明刘建新潘刚敏沈晓岚
Owner ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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