189 results about "Carbohydrate chains" patented technology

A medical composition is provided which is advantageous as a sealant for wound openings as well as have a function promoting healing of intractable wound or incision wound, and which accelerate tissue regeneration but does not cause any side effect such as canceration. The present invention relates to a medical composition comprising a photo-crosslinkable chitosan derivative and a wound healing promoter. The photo-crosslinkable chitosan derivative is preferably a polymer obtainable by incorporating a carbohydrate chain containing a reducing terminal to at least one portion of amino groups of chitosan back bone and incorporating a photoreactive group to at least another part of the amino groups. The wound healing promoter is preferably a growth factor.

The invention relates to a method for detecting low-molecular-weight heparin by combining ion pair reversed phase chronmatogaphy and mass spectrum. The method comprises the steps of: detecting all compositions of low-molecular-weight heparin by combining ion pair reversed phase chronmatogaphy and high-resolution mass spectrum, separating main compositions from a sample through the ion pair reversed phase chronmatogaphy, obtaining precise molecular weight by the high-resolution mass spectrum, and calculating information of a carbohydrate chain sequence, including structures of two ends, length of a carbohydrate chain and substitution number of ethanoyl and sulfate, thus carrying out fine surface features on the low-molecular-weight heparin. The method has great practical values on improving the heparin detection level and ensuring drug safety.

The invention provides a humanized modified anti-CD147 chimeric antibody HcHAb18 and an application thereof. The anti-CD147 human-rat chimeric antibody is prepared on the basis of obtaining hybridoma cells of an anti-human CD147 monoclonal antibody and antibody HcHAb18 genes of the hybridoma cells, wherein the variable region of light chains of the anti-CD147 human-rat chimeric antibody has the amino acid sequence shown in SEQ ID NO:1; the variable region of heavy chains of the anti-CD147 human-rat chimeric antibody has the amino acid sequence shown in SEQ ID NO:2; fucose are not combined in N-acetylglucosamine in a reducing end of a carbohydrate chain at a Fc segment of the anti-CD147 monoclonal antibody in a specific host cell; the glycoform of the anti-CD147 monoclonal antibody is of a mannose type (MAN 5); the anti-CD147 chimeric antibody has antibody dependent cell-mediated cytotoxicity (ADCC). The invention further provides an expression vector and an engineering cell strain of the anti-CD147 chimeric antibody and the application of the anti-CD147 chimeric antibody taken as a cancer treating medicine.

The invention provides a lectin test chip for a saliva sample, wherein a production cost is low, test targeting is strong, inherent advantages of a lectin chip are combined, and rapid, simple and efficient detection can be performed so as to obtain intermediate result information (saliva whole protein N-glycosylation and O-glycosylation carbohydrate chain structure information) adopted as type 2 diabetes mellitus evaluation. The lectin test chip adopts an epoxidation film base, and is characterized in that at least one of lectins such as PTL-I, LCA and SBA is immobilized on the epoxidation film base in a spotting immobilization manner. According to the present invention, the preparation method and the treatment method of the lectin test chip are optimized so as to establish the optimal reaction system for production of whole protein N-glycosylation and O-glycosylation information for saliva sample detection, and provide convenience for rapid and efficient detection of saliva glycoprotein carbohydrate chain structures of type 2 diabetes mellitus patients.

The invention relates to a group of glycosyl transferases and applications of the glycosyl transferases. Particularly, the invention provides applications of glycosyl transferases gGT25, gGT13, gGT30, gGT25-1, gGT25-3, gGT25-5, gGT29, gGT29-3, gGT29-4, gGT29-5, gGT29-6, gGT29-7, 3GT1, 3GT2, 3GT3 and 3GT4, and derived peptides of the glycosyl transferases in glycosylation catalysis and new saponin synthesis of terpenoids. The glycosyl transferases can specifically and efficiently catalyze hydroxyl glycosylation of C-20 bit and/or C-6 bit and/or C-3 of a tetracyclic triterpenoid compound substrate, and/or transfers the glycosyl groups from glycosyl donors to the first glycosyl groups of the C-3 bit and C-6 bit of the tetracyclic triterpenoid compound to extend a carbohydrate chain. The glycosyl transferases disclosed by the invention can also be applied to building synthetic rare ginsenosides and a plurality of novel ginsenosides and derivatives of the ginsenosides.

The relates to an agglutinin chip used for identifying cancer, in particular to an agglutinin chip for identifying breast cancer based on sialoprotein, a reagent kit and application of the reagent kit, and aims at providing an agglutinin chip for identifying breast cancer in a non-destructive mode through the change of carbohydrate chains in saliva, a reagent kit and application of the reagent kit. The agglutinin chip for identifying breast cancer based on sialoprotein comprises an agglutinin testing probe set. The agglutinin testing probe set at least comprises a combination of PHA-E+L, LTL, BS-I, MAL-I, LCA, BPL, PTL-II, NPA and GSL-I, or at least comprises a combination of LTL, BS-I, MAL-I, LCA, BPL, NPA and GSL-I. By means of the agglutinin chip, differentially-expressed glycoprotein carbohydrate chain structures in a patient saliva sample can be rapidly detected, whether a corresponding person suffers from a breast tumor or not is detected, and whether the breast tumor is breast cancer or not is determined; besides, saliva is adopted as an object to be detected and is easy and convenient to collect, and health risks existing when a serum sample is collected for detection in the prior art are avoided.

The invention relates to a lectin microarray for detecting a carbohydrate chain marker based on sialoprotein and a detection method of the carbohydrate chain marker using the same. The lectin microarray can nondestructively detect the carbohydrate chain marker by distinguishing sialoprotein. The lectin microarray comprises a testing lectin probe group, wherein the testing lectin probe group comprises a vicia villosa lectin, an aleuria aurantia lectin, a pea lectin, a winged bean lectin-II an a winged bean lectin-I, and the winged bean lectin-II and the winged bean lectin-I are used as internal reference lectins. The lectin microarray has the advantages that the human body is not injured when the carbohydrate chain marker for detecting the carbohydrate chain marker is harmless to a human body, less samples are used, the detection speed is high and the sensitivity is high, and the lectin microarray can be applied to identification of stomach cancer.

The invention relates to a diagnostic marker for systemic lupus erythematosus, and in particular discloses application of a reagent for specific detection of high mannose type N-linked carbohydrate chain level in a serum antibody to preparation of a diagnostic tool for diagnosis and/or prognosis assessment of systemic lupus erythematosus. The increase of the high mannose type N-linked carbohydrate chain level in the serum antibody is closely related to the occurrence of systemic lupus erythematosus. Therefore, the diagnostic marker can be used for early diagnosis and/or prognostic assessment of systemic lupus erythematosus, and further can be used for guiding treatment of systemic lupus erythematosus.

The invention discloses codonopsis pilosula uniform polysaccharide CPPib which is mainly composed of, by Mole percentage, 44.28% of rhamnose, 20.42% of galactose, 11.32% of arabinose and 23.97% of galacturonic acid, weight-average molecular weight of the codonopsis pilosula uniform polysaccharide CPPib is 1.45*105Da, and main carbohydrate chains are 1,2 connected rhamnose (rha, 45%), 1,4-connected galacturonic acid (GalA, 25%) and 1,2,6 connected galactose (Gal, 30%). A preparation method of the codonopsis pilosula uniform polysaccharide CPPib has the advantages of being simple, scientific, reasonable, and capable of preparing products with very high purity with only need of passing through an anion exchanging column chromatograph once. The prepared products have the advantages of having anti-tumor effect, educational effect, very high clinical application value and healthcare function.

The invention relates to a toughening forming auxiliary of a hard capsule taking starch as a matrix for food, healthcare products, medicines and the like, and a starch hard capsule for the food, healthcare products, medicines and the like, prepared from the toughening forming auxiliary of a hard capsule taking starch as a matrix in combination with a starch matrix. The toughening forming auxiliary for preparing a hard capsule taking starch as a matrix consists of 5-50wt% of carrageenan and 50-95wt% of ghatti gum. According to the toughening forming auxiliary provided by the invention, the toughening forming auxiliary is added into the starch matrix, so that the glue-dip forming is assisted by the gelation effect of the carrageenan, and a final capsule shell is toughened by the net structure generated in gelation and a natural polysaccharide carbohydrate chain structure in the ghatti gum; meanwhile, the disintegration of the hard capsule can be effectively promoted by the good solubility and emulsifiability of the ghatti gum, thus the prepared starch hard capsule has excellent flexibility and can be quickly disintegrated.

The invention relates to a screening method of dipeptide functional monomers having excellent separating capacity on saccharide. A dipeptide compound obtained on the basis of screening of standard deviation parameter D and R values is constructed, the dipeptide compound obtained through screening is further coupled with a group containing double bonds, polymerizable dipeptide functional monomers are obtained and are polymerized on surfaces of various matrixes, and a series of polymer modified chromatographic stationary phase materials are obtained. The materials have excellent separating capacity on oligomeric galactose, oligomeric fructose and sialic acid derivatives; meanwhile, the materials have excellent selective enrichment performance on the glycopeptide and separating capacity on a carbohydrate chain structure, have very high glycopeptide recovery rate and repeatability and have the wide application prospect in the fields of posttranslational modification proteomics research and the like.

The invention discloses polygonatum kingianum homogeneous polysaccharide and a preparation method and application thereof, the total sugar content of the polygonatum kingianum homogeneous polysaccharide is more than 95%, and the molecular weight of the homogeneous polysaccharide is 1-70kDa. The method for preparing the polygonatum kingianum homogeneous polysaccharide comprises the following steps:taking dried polygonatum kingianum, performing degreasing, extracting, precipitating and drying to obtain a polygonatum kingianum polysaccharide crude product; dissolving the obtained polygonatum sibiricum polysaccharide crude product with distilled water, loading the solution to an anionic cellulose chromatographic column, and performing eluting with distilled water to obtain polygonatum sibiricum washing component polysaccharide; purifying the obtained washing component polysaccharide by using a gel chromatographic column, and performing eluting by using a NaCl solution to obtain the polygonatum kingianum homogeneous polysaccharide. The polygonatum kingianum homogeneous polysaccharide is applied to an in-vitro antioxidant process. According to the invention, polygonatum sibiricum homogeneous polysaccharide is extracted and separated from polygonatum sibiricum for the first time and is subjected to carbohydrate chain sequencing. The preparation method is simple, high-purity homogeneous polysaccharide can be rapidly obtained, and the preparation method is suitable for large-scale production.

The invention belongs to the technical field of glycobiology, and particularly relates a method of the target board derivatization of a reducible carbohydrate chain. The method comprises the following steps: preparing a reducible carbohydrate solution, a Girard reagent T solution and a matrix DHB solution separately, and applying the matrix DHB solution, the reducible carbohydrate solution and the Girard reagent T solution at the same point of an MALDI-TOF-MS instrument target board separately; placing the target board subjected to sample application at room temperature till a sample point is completely dried. The invention further provides an analysis method of the MALDI-TOF-MS of a sample subjected to derivatization. The method is high in derivatization efficiency, can effectively prevent the degradation of acid carbohydrate chains, can detect both neutral carbohydrate chains and acid carbohydrate chains in a positive ion mode without switching, and is high in universality and simple and more efficient.

The invention discloses a method for efficiently and mildly reducing ovalbumin allergenicity. By repeated freezing and thawing pretreatment, ovalbumin allergenic parts are exposed to molecule surfaces, and by combination of transglycosylase and trypsin for synergistic treatment, ovalbumin carbohydrate chains and epitopes can be reduced, and ovalbumin and egg food allergenicity can be remarkably reduced. Compared with other traditional methods for reducing egg allergenicity by synergy of high-temperature high-pressure physical methods and an enzymic method, the method has advantages that egg allergenicity elimination pertinence and effectiveness are improved remarkably while egg white nutrition and quality damages are reduced to the maximum extent, thereby being an efficient and mild egg allergenicity elimination method. The method has a prospect in application to industrial development of low-allergenicity egg products.

The invention provides a lectin chip for detecting carbohydrate chain markers based on protein in blood serum or saliva as well as a kit and application of the lectin chip, relates to the lectin chip for detecting the carbohydrate chain markers in blood serum or saliva, and particularly relates to the lectin chip for detecting the carbohydrate chain markers based on protein in blood serum and saliva as well as a kit and application of the lectin chip. The invention aims at providing a non-injury lectin chip for detecting the carbohydrate chain markers by identifying protein in saliva or blood serum and a method thereof. The lectin chip for detecting the carbohydrate chain markers based on serum carbohydrate binding protein, provided by the invention, comprises a testing lectin probe set, wherein the testing lectin probe set at least comprises PTL-I, PNA, AAL, LTL, STL, BS-I, PTL-II, SBA, ACA, UEA-I, MAL-I and PHA-E+L. The lectin chip can be used for rapidly detecting a specific carbohydrate protein chain structure in blood serum and saliva samples, and rapidly judging the specific combination of carbohydrate chains in the samples, so as to determine whether corresponding people have hepatitis or not and determine whether people are ACHBLF patients or not.

The invention discloses application of lectin group recognized carbohydrate chains in distinguishing mucinous cystic neoplasm from serous cystic neoplasm. The lectin group recognized carbohydrate chains disclosed by the invention are glycosylated protein carbohydrate chains recognized by lectin groups consisting of WGA (trticum vulgaris agglutinin), BPL (bauhinia purpurea lectin), STL (solanum tuberosum (potato) lectin), DBA (dolchos biflorus agglutinin), PTL-I (psophocarpus tetragonolobus lectin I) and MAL-I (maackia amurensis lectin I). The carbohydrate chains are different in capsula pancreatic fluid of patients suffering from mucinous cystic neoplasm and serous cystic neoplasm, the contents of the carbohydrate chains recognized by STL, WGA, BPL and DBA in the capsula pancreatic fluid of patients suffering from MCN (mucinous cystic neoplasm) are remarkably higher than those in the capsula pancreatic fluid of patients suffering from SCN (serous cystic neoplasm), the contents of the carbohydrate chains recognized by PTL-I and MAL-I in the capsula pancreatic fluid of the patients suffering from MCN are remarkably lower than those in the capsula pancreatic fluid of the patients suffering from SCN, the sensitivity of the combination of WGA and BPL in distinguishing the patients suffering from SCN from the patients suffering from MCN is 0.714, and the specificity is 1. The results show that the lectin group can be used for distinguishing the patients from SCN from the patients suffering from MCN.

The invention relates to a composition for separating and detecting an exotic protein of a carbohydrate chain, a kit, as well as a method and application of the composition. The diagnosis conditions of the tumor caused by the exotic protein of the carbohydrate chain and the other related diseases are indicated according to the method, and the method is highly sensitively, and is rapid, convenientand automatic.

The invention belongs to the technical field of biology and relates to a method for multiply detecting a carbohydrate chain structure of glycoprotein through an antibody-assisted lectin liquid-phase suspension chip. According to the method, lectins are coupled to magnetic beads marked with different dyes, the magnetic beads which are coupled with different lectins are identified through a Bioplex detection system, and different glycoforms on the same protein can be simultaneously detected by adding the magnetic beads which are coupled with different lectins and have different serial numbers into a reaction, so that the detection flux of the carbohydrate chain structure is remarkably improved. According to the method, the carbohydrate chain structure on the glycoprotein can be quickly and specifically detected with high flux. The method can be further applied to detection of a clinical biological marker and provides an efficient way for research on disease glycomics.

The invention discloses preparation and application of a multi-component segmented hydrophilic copolymer-silica gel hybrid chromatographic packing material. The multi-component segmented hydrophilic copolymer-silica gel hybrid chromatographic packing material is generated after hydrophilic monomers like SBMA, GMAG and GMAM are polymerized on surfaces of silica gel particles in situ in sequence through a continuous surface-initiated atom transfer radical polymerization (SI-ATRP) method; the hybrid packing material is highly rough in surface, and further has a higher specific surface area; by utilizing solid phase extraction columns and high-performance liquid chromatographic columns filled with the multi-component segmented hydrophilic copolymer-silica gel hybrid chromatographic packing material, enrichment and separation of glycopeptides and carbohydrate chains in normalized glycoprotein and mouse liver complex samples can be successively realized, the selective enriching and separating effect is obvious, a total of 1244 glycopeptides and corresponding 577 glycoproteins are enriched and identified in mouse liver protein, and that the packing material has huge application value in glycosylation analysis of complex protein samples is fully proved.

The invention discloses a preparation method of an ultralow-molecular-weight pectin rich in RG-I. The preparation method comprises the steps that Fe<3+> generated in a reaction system is reduced into Fe<2+> through ultrasonic oxidation reaction, re-reaction between the Fe<2+> and hydrogen peroxide is strengthened to produce hydroxyl free radicals, oxidative cleavage of pectin carbohydrate chain is caused, and galacturonic acid in special attack pectin forms the low-molecular-weight pectin rich in RG-I structural domain. The weight-average molecular weight of the pectin is 5-10 kDa, esterification degree is reduced, the product is the ultralow-molecular-weight pectin rich in RG-I, the antioxidant activity is remarkably higher than that of original pectin, and the comprehensive utilization rate is improved.