41 results about "Protein chain" patented technology

The invention provides proteins from group B streptococcus (Streptococcus agalactiae) and group A streptococcus (Streptococcus pyogenes), including amino acid sequences and the corresponding nucleotide sequences. Data are given to show that the proteins are useful antigens for vaccines, immunogenic compositions, and / or diagnostics. The proteins are also targets for antibiotics.

The invention provides proteins from group B streptococcus (Streptococcus agalactiae) and group A streptococcus (Streptococcus pyogenes), including amino acid sequences and the corresponding nucleotide sequences. Data are given to show that the proteins are useful antigens for vaccines, immunogenic compositions, and / or diagnostics. The proteins are also targets for antibiotics.

The invention belongs to the technical field of biological information, and provides a protein binding site prediction method, device and equipment and a storage medium. The method comprises the stepsof receiving a protein sequence to be predicted, using a preset sliding window and a sliding step size for performing sequence classification on the protein sequence, obtaining a plurality of amino acid subsequences, constructing a word vector of the protein sequence according to the subsequences, performing document feature extraction on word elements, constructing document feature vectors of the protein sequence according to extracted document features, performing protein chain biological feature extraction on the amino acid subsequences, constructing biological feature vectors of the protein sequence according to the extracted biological features, adopting a preset amino acid residue group classification model for classifying the amino acid subsequences represented by the document feature vectors and the biological feature vectors, and obtaining an amino acid residue group type of the protein sequence; accordingly, the protein binding site prediction accuracy and applicability areimproved.

The invention discloses a monoclonal antibody MERS-4, as well as a coding gene and an application thereof. The antibody provided by the invention is named monoclonal antibody MERS-4, comprises a chain A and a chain B, wherein the chain A is (a) protein consisting of an amino acid sequence as shown in a sequence 1 or (b) protein which is derived from the sequence 1 by substituting and / or deleting and / or adding one or more amino acid residues on the sequence 1 and has the same function; and the chain B is (c) protein comprising an amino acid sequence as shown in a sequence 3 or (d) which is derived from the sequence 3 by substituting and / or deleting and / or adding one or more amino acid residues on the sequence 3 and has the same function. The monoclonal antibody can be used for effectively inhibiting MERS-CoV invasion cells, can be used as medicines for preventing or treating MERS-CoV, and has an important theoretical guiding value and a wide application prospect for prevention and treatment of MERS-CoV.

A method for producing feed for forming into pellets, the pellets produced by the method to be used to feed carnivorous animals. The addition of the enzyme transglutaminase to a feed mass specifically intended for carnivorous fish will catalyze a reaction between the amino acids glutamine and lysine which form part of the protein chains and the raw material of the proteins of the feed, such that a covalent chemical bond is formed between them, which results in shape permanence in the formed, dried, finished pellets, such that the finished pellets do not lose their shape before time of use.

This invention discloses a series of new amino acids modified by imidazoline and its synthesis method and application for polypeptide fixed-point marking. This invention prepares a series of new amino acids that contain 2-substitution-1,3-dioxy-imidazoline through linking 2-substitution-1,3-dioxy-imidazoline to alkaline side chain or alpha- amino of L-Lys or L-Arg, or linking to acidic side chain oralpha-oxatyl of L-Asp or L-Glu. The invented amino acids that are modified by imidazoline have following characteristics. 1) It has satisfactory chemical stability, can tolerance various kinds of reaction condition in process of polypeptide synthesis, can maintain nature of free radical or removing remove being not invariable, also can carry out fixed-point ESR marking like normal L- amino acid in the process of polypeptide synthesis according to random place of polypeptide or protein where need to be inserted; 2) It has satisfactory biocompatibility. Polypeptide that is obtained by amino acid fixed-point marking in this invention is compared with polypeptide obtained by N end marking in present technique, the physico-chemical property and biological activity of them is completely coincident.

The invention provides a lectin chip for detecting carbohydrate chain markers based on protein in blood serum or saliva as well as a kit and application of the lectin chip, relates to the lectin chip for detecting the carbohydrate chain markers in blood serum or saliva, and particularly relates to the lectin chip for detecting the carbohydrate chain markers based on protein in blood serum and saliva as well as a kit and application of the lectin chip. The invention aims at providing a non-injury lectin chip for detecting the carbohydrate chain markers by identifying protein in saliva or blood serum and a method thereof. The lectin chip for detecting the carbohydrate chain markers based on serum carbohydrate binding protein, provided by the invention, comprises a testing lectin probe set, wherein the testing lectin probe set at least comprises PTL-I, PNA, AAL, LTL, STL, BS-I, PTL-II, SBA, ACA, UEA-I, MAL-I and PHA-E+L. The lectin chip can be used for rapidly detecting a specific carbohydrate protein chain structure in blood serum and saliva samples, and rapidly judging the specific combination of carbohydrate chains in the samples, so as to determine whether corresponding people have hepatitis or not and determine whether people are ACHBLF patients or not.

The invention discloses an andrias davidianus secretion hydrogel, and a preparation method and an application thereof. Raw materials of the andrias davidianus secretion hydrogel comprise andrias davidianus secretions, acetic acid or an acetic acid solution, an alginate, and a solvent. Acetic acid small molecules can break interchain and intrachain ionic complexation and hydrogen bonds of an andrias davidianus secretion protein, and when the alginate is added, a cross-linked network is formed in short time through ionic complexation of amino groups from the protein chains and carboxylic groups from the alginate, so that the andrias davidianus secretion hydrogel still having good adhesion property in water is obtained. The andrias davidianus secretion hydrogel also has good self-healing ability, and can be extensively used for biological adhesives and preparation of medicines for treating wounds.

Genes and proteins involved in the biosynthesis of benzodiazepines by microorganisms, including the genes and proteins forming the biosynthetic loci for the benzodiazepine anthramycin from Streptomyces refuineus subsp. thermotolerans. The genes and proteins allow direct manipulation of benzodiazepines and related chemical structures via chemical engineering of the enzymes involved in the biosynthesis of anthramycin.

The invention provides a snake simulation method in protein folding simulation. (1) an initial state: serving as a straight protein sequence; (2) starting of folding: making wave motion and expressing by a function of sin(t); (3) later stage of folding: making temporary rectilinear motion and expressing by a function that x is equal to x plus t; and (4) end of folding: making coiling motion, and expressing by a function that x(t) is equal to (C<1>cos(omega t) plus C<2>sin(omega t)), wherein C and omega are coefficients and related to self characteristics of protein. For the protein with unknown space structure, by the snake simulation algorithm, an intermediate body and final space structure of a protein chain are predicted, thereby reducing human and material resources consumed in the process of measuring the space structure of the protein, and simultaneously providing folding analysis for theory researchers to refer.

Genes and proteins involved in the biosynthesis of benzodiazepines by microorganisms, including the genes and proteins forming the biosynthetic loci for the benzodiazepine anthramycin from Streptomyces refuineus subsp. thermotolerans. The genes and proteins allow direct manipulation of benzodiazepines and related chemical structures via chemical engineering of the enzymes involved in the biosynthesis of anthramycin.

A method for producing feed for forming into pellets, the pellets produced by the method to be used to feed carnivorous animals. The addition of the enzyme transglutaminase to a feed mass specifically intended for carnivorous fish will catalyze a reaction between the amino acids glutamine and lysine which form part of the protein chains and the raw material of the proteins of the feed, such that a covalent chemical bond is formed between them, which results in shape permanence in the formed, dried, finished pellets, such that the finished pellets do not lose their shape before time of use.

The invention discloses a dog interferon freeze-dried product, and relates to the technical field of freeze-dried biological products.The dog interferon freeze-dried product is prepared by freeze-drying a dog interferon stock solution and a freeze-drying protecting agent, wherein a freeze-drying protecting agent base solution is composed of a pH buffer agent and a protein protecting agent, and the freeze-drying protecting agent comprises a heat-resisting protective agent, a filling agent and a freeze-drying accelerating agent.The prepared dog interferon freeze-dried product can be stored for a long term at 2 DEG C-8 DEG C; due to the fact that the freeze-drying accelerating agent is added, the freeze-drying time of a freeze-drying technological link in large-scale industrialized production is shortened, the freeze-drying efficiency is improved, it is guaranteed that freeze-drying is full, and the stability of products of different batches is guaranteed; through combination of the preferable heat-resisting protective agent and filling agent, the anti-fracture strength of a dog interferon protein chain is improved, the biological activity of the dog interferon freeze-dried product is improved, and the product yield ratio of the dog interferon freeze-dried product is increased.

The invention discloses a protein interaction site identification method which belongs to the field bioinformatics analysis. The method comprises the steps of acquiring protein chain data, performing preprocessing on the protein chain data, and dividing the preprocessed protein chain data to an interface residue and a non-interface residue; extracting a characteristic from a database, fusing the extracted characteristics for obtaining a data set, processing the unbalance of the data set, then dividing the processed data set into a training set and a testing set, training the XGBoost model by means of the training set, and finally obtaining the protein interaction site by means of the XGBoost model. The protein interaction site identification method aims to overcome a defect of relatively high result analysis difficulty caused by different degrees of false positive and false negative characteristics in predicting the protein interaction site. The protein interaction site identification method can overcome defects above and furthermore can improve identification precision of the protein interaction site.

The invention provides an extended noodle making method. The extended noodle making method comprises the following steps that: a certain proportion of saline water is added into flour; stirring is carried out; the flour is kneaded into a dough which is uniformly fused and consistent in colour; the dough is repeatedly extended and pressed into a smooth and compact cake; the elasticity of the cake is further improved; the cake is pressed and rolled into a smooth, compact, uniform-thickness, hole-free and burr-free dough belt; the dough belt is rolled into uniform and smooth noodles with the diameter of about 0.8 cm by several times; then, the noodles are spun and wound into uniform-thickness noodles with the diameter of about 0.5 cm; fermentation is carried out for several times for a long time during the period, so that a protein chain is better stretched; the moulded dough is more chewy, elastic and smooth and has better stretchability; the noodles, which are manually stretched and drawn into fine noodles like silk threads, are dried at specific temperature and humidity; the dried extended noodles are cut and polished; crumbs and thorns on the surfaces of the noodles are removed through mutual friction among the noodles, so that the noodles are smoother; finally, due to manual fine selection, it is ensured that all batches of extended noodles are consistent in length, uniform in thickness, neat and smooth; the quality completely meets the standard; and each procedure of extended noodle production needs rich working experience of technicians, exquisite skills and meticulousand excelsior craftsmanship spirit.

The invention provides fish scale jelly instant food comprising fish scales, beef tallow fruit, fruit jar and yoghourt according to weight by parts: 15 to 25 parts of fish scales, 3 to 5 parts of beef tallow fruit, 3 to 5 parts of fruit jar and 25 to 50 parts of yoghourt. The beneficial effects are that high fish scale jelly output rate and great calcium level can be achieved; nutritious elements cannot be damaged; simple technology and convenient extraction can be achieved; low cost is required and fish scales can be fully utilized; high-temperature softening operation is conducted, so gelatineous big molecules fish scale collagen protein chains can be broken and partially decomposed into low-molecular protein peptide, thereby facilitating human absorption and improving product quality; with high-temperature constant-temperature deep-fry, fish scales can quickly blister and swell, so the instant food is crispy and tasty, and product taste and nutrients can be remained; and with yoghourt mixture and fermentation, taste of the fish scale jelly can be adjusted and the jelly can be more popular.

A novel spinning technology is used for spinning zein and fibroin, the spinning technology is simple, an electric field does not need to be used, and the diameter of the prepared nano fiber is much smaller than the diameter of electrostatic spinning fiber and can reach 200-350 nm. Since a silane coupling agent is adopted for grafting of nano titania to a protein chain, aggregation of inorganic particles is avoided, and dispersibility of the inorganic particles is better improved; meanwhile, zinc sulfide and titanium oxide serve as anti-microbial materials, when the zinc sulfide and the titanium oxide are jointly used, the long anti-microbial effect is achieved, the anti-microbial performance can reach 99% or above, good anti-microbial performance is still achieved after washing is conducted 40 times, and the anti-microbial rate can still reach 90% or above.

The invention discloses a monoclonal antibody MERS‑4, its coding gene and application. The antibody provided by the present invention, named monoclonal antibody MERS‑4, consists of chain A and chain B; chain A is (a) or (b): (a) a protein consisting of the amino acid sequence shown in Sequence 1; ( b) A protein derived from Sequence 1 that undergoes substitution and / or deletion and / or addition of one or several amino acid residues and has the same function; B chain is (c) or (d): (c) is composed of A protein composed of the amino acid sequence shown in Sequence 3; (d) a protein derived from Sequence 3 that undergoes substitution and / or deletion and / or addition of one or several amino acid residues in Sequence 3 and has the same function. The monoclonal antibody provided by the invention can effectively inhibit MERS-CoV from invading cells, can be used as a preventive or therapeutic drug for MERS-CoV, and has important theoretical guiding value and broad application prospects for the prevention and treatment of MERS-CoV.

This invention discloses a series of new amino acids modified by imidazoline and its synthesis method and application for polypeptide fixed-point marking. This invention prepares a series of new aminoacids that contain 2-substitution-1,3-dioxy-imidazoline through linking 2-substitution-1,3-dioxy-imidazoline to alkaline side chain or alpha- amino of L-Lys or L-Arg, or linking to acidic side chainoralpha-oxatyl of L-Asp or L-Glu. The invented amino acids that are modified by imidazoline have following characteristics. 1) It has satisfactory chemical stability, can tolerance various kinds of reaction condition in process of polypeptide synthesis, can maintain nature of free radical or removing remove being not invariable, also can carry out fixed-point ESR marking like normal L- amino acidinthe process of polypeptide synthesis according to random place of polypeptide or protein where need to be inserted; 2) It has satisfactory biocompatibility. Polypeptide that is obtained by amino acid fixed-point marking in this invention is compared with polypeptide obtained by N end marking in present technique, the physico-chemical property and biological activity of them is completely coincide nt.

The invention relates to a novel hetero-dimeric multi-specific format of multiple antibody variable domains comprising a core of two split variable domain pairs wherein both variable light domains and the two cognate variable heavy domains are positioned in tandem on two separate protein chains, respectively.

The invention provides a batch generating method of a pharmacophore model. The method comprises the steps of automatically downloading a PDB file of a protein complex structure in a PDB database through a computer script, automatically identifying and keeping the protein complex required in the file; calling a chain required for combination of the protein complex from a virtual file database, andconverting the acquired protein sequence to a FASTA format; then obtaining automatic segmentation and recombination information according to adjacent amino acid information and combinable informationbetween different protein chains which are called from the PDB file, thereby forming a new SD file and a PDB file; and finally through self analysis of the script, performing batch generation of the required pharmacophore model, thereby realizing construction of the pharmacophore model between the proteins. The whole operation is finished in a full automatic manner. Furthermore the method is established based on the PDB file data of biology. Furthermore high accuracy and high efficiency are realized in model establishment.

A virtual theme park has attractions which are based upon protein chains at large scale, the users being able to use these protein tracks with virtual transports such as roller coasters, water rides, ski rides, skate scooters, skate boards, racing cars, bicycles, motorcycles, etc. The proteins are those proteins including pathogens which representing diseases of animals or humans. The virtual riders may be presented as the disease vector or target, for instance a mosquito to navigate a dengue fever related protein, a cow to navigate a BSE prion. The motion effects and sound effects are modelled based on the protein structure.

This invention relates to novel hetero-dimeric multi-specific format of multiple antibody variable domains comprising a core of two split variable domain pairs wherein both variable light domains andthe two cognate variable heavy domains are positioned in tandem on two separate protein chains, respectively.

Multi-chain proteins can be produced in a subject by intramuscular injection of one or more vectors encoding the protein chains, optionally by applying one or more electrical pulses at the injection site. Preferred multi-chain proteins are immunoglobulins. Methods of generating antibodies have diverse applications, including in vivo expression of multi-chain proteins for the treatment of disease, and can also be used to elicit an immune response against one or more foreign epitopes of the expressed protein.

This invention relates to novel hetero-dimeric multi-specific format of multiple antibody variable domains comprising a core of two split variable domain pairs wherein both variable light domains and the two cognate variable heavy domains are positioned tandem on two separate protein chains, respectively.

The invention discloses an andrias davidianus secretion hydrogel, and a preparation method and an application thereof. Raw materials of the andrias davidianus secretion hydrogel comprise andrias davidianus secretions, acetic acid or an acetic acid solution, an alginate, and a solvent. Acetic acid small molecules can break interchain and intrachain ionic complexation and hydrogen bonds of an andrias davidianus secretion protein, and when the alginate is added, a cross-linked network is formed in short time through ionic complexation of amino groups from the protein chains and carboxylic groups from the alginate, so that the andrias davidianus secretion hydrogel still having good adhesion property in water is obtained. The andrias davidianus secretion hydrogel also has good self-healing ability, and can be extensively used for biological adhesives and preparation of medicines for treating wounds.

The invention discloses protein fibers capable of being prepared on a large scale and application thereof. A method for preparing the protein fibers on a large scale comprises the following steps that protein, a tris (2-carboxyethyl) phosphine hydrochloride aqueous solution and a spinning solvent are uniformly mixed, and then a protein chain is unfolded to form a spinning solution; the spinning solution is extruded into a coagulating bath from a spinning nozzle under the driving force of an injection pump to assemble a large number of primary protein fibers with amyloid-like structures, and the primary protein fibers are stretched and dried to form protein fibers with excellent mechanical properties. The preparation method can also be used for producing protein hollow fibers for cell culture. Besides, the primary protein fibers are soaked in a graphene oxide dispersion liquid, stretched and dried and then placed in the tris (2-carboxyethyl) phosphine hydrochloride aqueous solution to be reduced to obtain stable conductive protein fibers, and the conductivity of the conductive protein fibers is not changed even under the action of repeated ultrasonic cleaning and 3M adhesive tape tearing. The conductive protein fibers can monitor the stress effect and the temperature and humidity change in real time, and has the potential application of wearable intelligent fabric.

The present invention is applicable to the technical field of biological information, and provides a method, device, equipment, and storage medium for predicting protein binding sites. The method includes: receiving a protein sequence to be predicted, and using a preset sliding window and sliding step The sequence is divided into sequences to obtain multiple amino acid subsequences, the word vector of the protein sequence is constructed according to these amino acid subsequences, the document feature extraction is performed on the word elements, and the document feature vector of the protein sequence is constructed according to the extracted document features, and these amino acid subsequences are Extract the biological features of the protein chain, construct the biological feature vector of the protein sequence according to the extracted biological features, and use the preset amino acid residue classification model to classify the amino acid subsequence represented by the document feature vector and biological feature vector, The amino acid residue type of the protein sequence is obtained, thereby improving the accuracy and versatility of protein binding site prediction.

The invention provides a snake simulation method in protein folding simulation. (1) an initial state: serving as a straight protein sequence; (2) starting of folding: making wave motion and expressing by a function of sin(t); (3) later stage of folding: making temporary rectilinear motion and expressing by a function that x is equal to x plus t; and (4) end of folding: making coiling motion, and expressing by a function that x(t) is equal to (C<1>cos(omega t) plus C<2>sin(omega t)), wherein C and omega are coefficients and related to self characteristics of protein. For the protein with unknown space structure, by the snake simulation algorithm, an intermediate body and final space structure of a protein chain are predicted, thereby reducing human and material resources consumed in the process of measuring the space structure of the protein, and simultaneously providing folding analysis for theory researchers to refer.

The invention discloses a dog interferon freeze-dried product, and relates to the technical field of freeze-dried biological products.The dog interferon freeze-dried product is prepared by freeze-drying a dog interferon stock solution and a freeze-drying protecting agent, wherein a freeze-drying protecting agent base solution is composed of a pH buffer agent and a protein protecting agent, and the freeze-drying protecting agent comprises a heat-resisting protective agent, a filling agent and a freeze-drying accelerating agent.The prepared dog interferon freeze-dried product can be stored for a long term at 2 DEG C-8 DEG C; due to the fact that the freeze-drying accelerating agent is added, the freeze-drying time of a freeze-drying technological link in large-scale industrialized production is shortened, the freeze-drying efficiency is improved, it is guaranteed that freeze-drying is full, and the stability of products of different batches is guaranteed; through combination of the preferable heat-resisting protective agent and filling agent, the anti-fracture strength of a dog interferon protein chain is improved, the biological activity of the dog interferon freeze-dried product is improved, and the product yield ratio of the dog interferon freeze-dried product is increased.