12 results about "Protein chain" patented technology

A complex bait data set construction method based on structure and sequence collaborative redundancy elimination belongs to the field of bioinformatics, and comprises the following steps: screening an initial protein complex structure set, removing entries containing nucleic acids, small molecules or non-protein chains, and selecting binary complexes meeting integrity and resolution requirements; secondly, structure clustering and sequence clustering are carried out based on three-dimensional structure similarity and sequence homology, combined comparison is carried out on the two results, and redundant compound entries which are highly similar in structure and sequence are removed; then, taking each cluster representative compound as a target, generating a plurality of groups of bait structures by using a molecular docking or prediction modeling method, and calculating a quality index; and finally, performing stratified sampling and proportion balance based on the score interval of the quality index, and constructing a high-quality protein complex bait data set with structure and sequence collaborative redundancy elimination and balanced quality distribution. The data set generated by the method has the advantages of low redundancy, high diversity and quality distribution controllability.

The invention provides a phosphorus-fixing lanthanum-based nano composite material as well as a preparation method and application thereof, and relates to the field of novel nano composite materials. According to the material, lysozyme protein fibers are taken as a structural template, a confinement space is constructed by utilizing the ultrahigh length-diameter ratio of the lysozyme protein fibers, and controllable synthesis of the sub-5nm high-dispersion lanthanum oxide nanoparticles is realized. Functional groups in a protein chain can be enriched and nucleation sites can be provided through electrostatic attraction and key coordination synergistic effect, so that the lanthanum-based protein fiber nano composite material is formed. When the material is used for treating phosphorus wastewater, efficient phosphorus removal and long-term phosphorus fixation are realized through an enrichment-conversion synergistic mechanism: phosphate ions are enriched through electrostatic adsorption, and thermodynamically stable lanthanum phosphate is generated in situ at lanthanum active sites. According to the method, gt is still kept in the presence of high-concentration equal competitive ions; the phosphorus removal rate is 90%, the generated lanthanum phosphate can be used as a slow-release phosphate fertilizer or an industrial phosphorus chemical raw material, and closed-loop conversion from pollution purification to resource utilization is realized.

The application discloses a kind of amyloid-like protein-mediated long-acting metal organic framework antifouling agent and preparation method and application thereof, and belongs to the field of marine antifouling agent material preparation.The antifouling agent is based on MOF with antifouling function, and amyloid-like protein self-assembles to form a dense protective shell layer on the surface of MOF.In the preparation method, metal ions and organic ligands are connected by coordination bond to form MOF structure, and MOF particles are obtained by centrifugation, collection, precipitation, washing and drying;under the action of water-soluble organic reducing substance, the disulfide bond of protein is completely opened to form unfolded protein chain, and amyloid-like protein solution is obtained by self-assembly;MOF particles are incubated in amyloid-like protein solution by simple one-step method to obtain amyloid-like protein-mediated long-acting metal organic framework antifouling agent.The application uses a new method of green, high stability, fast and efficient mild modification to prepare antifouling agent, which has stability, durability, universality and high efficiency.

The invention discloses a natural spider silk-based self-adaptive surgical suture and a preparation method thereof, and belongs to the technical field of biomedical materials. According to the surgical suture, natural spider silk serves as a raw material, protein chains in the spider silk are rearranged in order by regulating and controlling the moisture state, applying axial pre-stretching strain and conducting drying, annealing and shaping under the limited condition, and the suture material with the high strength and the environmental response characteristic is obtained. The surgical suture is based on natural spider silk, has excellent biocompatibility and inherent biodegradability, becomes an ideal candidate material for biomedical application, and is successfully applied to suture of skin, stomach and other parts. According to the invention, the spider silk protein chains are rearranged into the superfine nanofibers, so that the engineered natural spider silk suture with remarkably enhanced mechanical and functional properties can be produced, and the monofilament can realize the breaking strength of 2.0 GPa, the toughness of 480 MJ m < 3 > and the driving stress of 92.6 MPa.

The invention discloses a self-assembled triblock piezoelectric peptide, a preparation method thereof and application of the self-assembled triblock piezoelectric peptide in preparation of a high-performance piezoelectric material. The self-assembled triblock piezoelectric peptide is prepared from 3, 3-diphenylalanine (FF), a silk protein chain segment GAGAGS and tyrosine tripeptide YYY, and is characterized in that the self-assembled triblock piezoelectric peptide is prepared from the following raw materials: a polypeptide A and a polypeptide B; a 3, 3-diphenylalanine piezoelectric group is introduced to one end of the piezoelectric peptide, a GAGAGS silk protein chain segment structure is introduced to the middle of the piezoelectric peptide, and a hydrophilic chain segment of tyrosine tripeptide is introduced to the other end of the piezoelectric peptide; by regulating and controlling the temperature or pH and the like, self-assembly is carried out to form a highly-ordered nanofiber (tubular or linear nanofiber) structure, so that the piezoelectric peptide with remarkably enhanced piezoelectric property is obtained; furthermore, the piezoelectric peptide obtained by self-assembly is added into different medical polymer materials, so that a high-performance piezoelectric material is prepared, and the high-performance piezoelectric material has good biocompatibility and piezoelectric performance and has wide application prospects in the fields of flexibility and biomedicine.

The invention relates to the technical field of protein antigen epitope prediction, in particular to a deep learning method and system for protein antigen epitope prediction, and the method comprises the steps: collecting a protein chain with epitope annotation from a SabDab database, and constructing a data set for learning training; a three-dimensional structure of the protein is represented as a graph structure, nodes of the graph structure are amino acid residues, and edges are determined by spatial distances among the amino acid residues; taking the graph structure as input based on the data set, and pre-training to obtain a VQVAE model; and processing the graph structure according to an encoder, extracting node features as input to train a GT model, performing antigen epitope prediction based on the data set, and outputting the probability that each amino acid residue belongs to the antigen epitope. According to the method, key limitations in the aspects of feature characterization, long-range dependence modeling and data utilization are effectively solved, so that the prediction accuracy of the protein antigen epitope is remarkably improved on the whole.

The invention belongs to the technical field of formula food for special medical purposes, and particularly relates to liquid special medical nutritional food containing whey protein and a preparation method of the liquid special medical nutritional food. The food is prepared from the following raw materials: maltodextrin, whey protein powder, phospholipid, citric acid fatty glyceride, stevioside, gellan gum, sodium carboxymethyl cellulose, microcrystalline cellulose, sunflower seed oil, rapeseed oil, medium chain triglyceride, macro minerals, a vitamin premix, a trace mineral premix, a functional premix, food essence and water. The pre-denatured whey protein powder accounting for 50%-100% of the total amount of protein is introduced, hydrophobic groups are exposed in advance, part of protein chains are effectively broken, a compact gel network formed by hydrophobic interaction and disulfide bonds among protein molecules in the subsequent high-temperature sterilization process is reduced, and thermal aggregation precipitation of the protein is inhibited from the source; the compounded stabilizer system and the pretreated protein have a synergistic effect to jointly construct a stable colloid system.

The invention relates to the technical field of gargle preparation, in particular to an oral gargle based on synthetic biological materials. Comprising the following steps: preparing a pre-crosslinked mussel protein adhesion network precursor solution, preparing a temperature-sensitive gel matrix, preparing a liquid main agent, preparing a nano-emulsion and preparing the oral gargle based on the synthetic biological material. Through sequential combination of tea polyphenol pretreatment and immobilized tyrosinase catalysis, the recombinant mussel byssus protein is subjected to directional chemical modification, in a weakly alkaline buffer solution, a catechol structure of tea polyphenol can be combined with a protein chain to play a role in pre-organization and stabilization, and the tea polyphenol plays a role in improving the stability of the mussel byssus protein while participating in network construction. The inherent antibacterial and antioxidant functions are integrated into a material matrix in situ, a mechanical stability foundation is created for the subsequent compounding of a protein network and temperature-sensitive hydrogel, the uniform coexistence of a protein phase and a hydrogel phase is ensured, and the wet adhesion strength of the protein material is essentially improved.

The invention belongs to the technical field of structural biology, and provides a cryoelectron microscope structure database building method and system and a storage medium, and the method comprises the steps: obtaining an input structure of each density map based on EMDB density maps; adjusting a protein chain in the input structure based on the density map to obtain a template structure; based on the density map, a protein sequence and unmodeled annotations in an input structure are adjusted, and the protein sequence and the unmodeled annotations matched with the template structure are obtained; performing structure prediction and optimization based on the density map, the protein sequence and the template structure to obtain a candidate structure; calculating a model-density map correlation coefficient of the candidate structure and the density map, and adjusting the candidate structure to obtain a final structure; and performing structure evaluation on the final structure, generating a structure annotation in combination with a protein sequence and an unmodeled annotation, and importing the final structure and the structure annotation into a database. According to the construction method of the cryoelectron microscope structure database, the structure quality in the database is improved.

The invention discloses a protein electrostatic spinning fibrous membrane dressing for promoting scar-free healing of wounds. The protein electrostatic spinning fibrous membrane dressing comprises water-soluble globular protein, a disulfide bond reducing agent and a stabilizer (trifluoroethanol, a urea water solution, trifluoroacetic acid and the like). According to the dressing, disulfide bonds of water-soluble globular protein are broken through a disulfide bond reducing agent to form an expanded protein chain, and then the expanded protein chain is stabilized through a stabilizer; electrostatic spinning is carried out by controlling the direct-current voltage between the spray head and the roller receiver rotating at a high speed, the receiving distance and the rotating speed of the roller receiver, and the protein nanofiber membrane dressing with the oriented arrangement structure is prepared. The protein nanofiber membrane dressing inhibits excessive wound contraction by reducing expression of alpha-SMA, promotes wound healing at the same time, and has a remarkable anti-scar effect. The dressing has a wide application prospect in wound treatment, especially in clinical application of pursuing scar-free healing.

This invention discloses a biotechnology-based process and product for preparing deodorized mushroom concentrate, relating to the field of mushroom concentrate technology. The invention involves taking finely filtered mushroom broth, concentrating it, and controlling the solid content to obtain a mushroom concentrate. The mushroom concentrate is then adjusted to pH using sodium hydroxide or hydrochloric acid, heated, and immobilized bromelain and papain are added, stirred, and then immobilized flavor protease is added, stirred, and filtered to obtain the deodorized mushroom concentrate. Bromelain, papain, and flavor protease are selected. Bromelain and papain are endopeptidases that cleave long protein chains in mushrooms at different sites, providing more ends for the flavor protease. The flavor protease is an exopeptidase that hydrolyzes the protein fragments cleaved by bromelain and papain to form flavor amino acids. During the enzymatic hydrolysis process, the immobilized carriers of the three enzymes remove off-odors through adsorption.

This invention discloses a system, technology, and material composition for correcting eye condition. The system includes a processing device and an etching device. The processing device includes a pattern correction module configured to provide a selected two-dimensional pattern based on visually non-permanent input data instructing a user, and configured to generate operating instructions to form the selected two-dimensional pattern on the surface of the cornea via the etching device. The etching device is configured to etch the selected pattern onto the surface of a user's cornea. The material composition includes an aqueous solution containing a plurality of nanoparticles, the nanoparticles comprising magnetite nanoparticles encapsulated by biocompatible protein chains. The material composition can be used as eye drops, thereby allowing the nanoparticles to occupy the etched area on the cornea, thus maintaining the correction of eye condition.