A lectin chip and method for detecting sugar chain markers based on salivary protein

Abstract

A lectin chip for detecting sugar chain markers based on salivary protein and a method thereof, relating to a lectin chip for detecting sugar chain markers, in particular to a lectin chip for detecting sugar chain markers based on protein and its method. The purpose is to provide a non-damaging lectin chip and method for detecting sugar chain markers by identifying proteins in saliva. The lectin chip for detecting sugar chain markers based on salivary protein includes a test lectin probe set, and the test lectin probe set includes faba bean lectin, Reticula aurantia lectin, pea lectin, square bean Lectin-II and lentil bean lectin-I, wherein lentil lectin-II and lentil bean lectin-I were used as internal reference lectins. Using the lectin chip to detect sugar chain markers has the advantages of no damage to the human body, less sample consumption, fast detection results and high sensitivity, and can be applied to identify whether there is gastric cancer.

Description

technical field [0001] The invention relates to a lectin chip for detecting sugar chain markers, in particular to a protein-based lectin chip for detecting sugar chain markers and a method thereof. Background technique [0002] Gastric cancer is a common malignant tumor with high morbidity and mortality worldwide, especially in East Asia, Eastern Europe, and South America due to the lack of significant early features. Currently commonly used diagnostic markers for gastric cancer include: CEA, CA19-9, CA72-4, CA125, AFP, etc., but all have limitations. Histopathological examination is still the gold standard for the diagnosis of gastric cancer at present. However, in view of the inherent defects of histological examination, such as destructive examination, dynamic detection, and sampling differences, it is important to seek non-invasive examination for early diagnosis of gastric cancer. The key to gastric cancer. [0003] With the progress of society and the development of ...

AI Technical Summary

Problems solved by technology

[0006] For example, some functional sugar chains have been found in the research of gastric cancer, and the representative one is the up-regulated expression of N-acetylglucosaminyltransferase V (GnT-V) in gastric cancer cells, which leads to the synthesis of β1,6-linked N-acetyl Glucosamine branched N-glycan (β1,6GlcNAc branched N-Glycan), this branched sugar chain affects the phosphorylation of tyrosine in catenin, resulting in the damage of β-catenin and P120 protein, destroying catenin The stability of the combination with N-sugar chains affects the adhesion ability between cells and increases the ability of cell transfer and invasion

Images