16 results about "Aedes albopictus" patented technology

The invention discloses an insect repellent. The insect repellent provided by the invention comprises anthranilate and deet. The invention also provides an insect repellent spray which comprises the insect repellent, an organic solvent and water. The insect repellent provided by the invention is applied to preparation of products for repelling pests. The mosquito and tick repellent is a mixed preparation of anthranilate and deet, and can play a role in enhancing the effect of repelling mosquitoes and ticks; the preparation can be applied to environments with high mosquito and tick density, such as humid and hot forests, lakesides, grassland, marshes and the like with dense vegetation, is suitable for outdoor mountain climbing, exploration, fishing, picnic and field farming activities, can be sprayed to skin or clothes, can effectively prevent various mosquito and tick bites, and has a good application prospect. Comprise aedes aegypti, aedes inside, aedes albopictus, culex pipicorus, culex modestus, haemaphysalis unguiculata, picetophysalis katsumadai and the like.

The invention relates to a diptera adult insecticide, which contains an insecticidal functional component n-butyl alcohol. According to the invention, n-butyl alcohol which has an obvious poisoning effect on the adult cucurbita petiolatus is separated from a metabolite of a bacillus thuringiensis XLB2910 strain, and 2, 3, 5-trimethylpyrazine and benzothiazole which have an obvious poisoning effect on the adult aedes albopictus are also separated from the metabolite of the bacillus thuringiensis XLB2910 strain. Through poisoning concentration analysis of different components and a compound combination experiment, a series of pesticides for adult cucurbita petioles and adult aedes albopictus are formed, the prepared compound pesticide for cucurbita petioles comprises 2.5%-10% of 2, 3, 5-trimethylpyrazine, 2%-10% of benzothiazole and 5%-10% of n-butyl alcohol, and the prepared compound pesticide for aedes albopictus comprises 2.5%-10% of 2, 3, 5-trimethylpyrazine, 2%-10% of benzothiazole and 5%-10% of n-butyl alcohol. The composition comprises 0.5%-3% of 1, 3, 5-trimethylpyrazine, 0.05%-1.5% of benzothiazole and 1%-3% of n-butyl alcohol. The compound insecticide has a good insecticidal effect on cucurbita petiolatus adults and aedes albopictus adults, and is wide in raw material source and environment-friendly.

The invention belongs to the technical field of mosquito repelling, and particularly relates to application of Badong buddleia alcohol extract in preparation of a mosquito repelling agent. The alcohol extract of Badong buddleia has a repelling effect on aedes albopictus, the Badong buddleia Soxhlet alcohol extract with the concentration of 0.2 g / mL has the best repelling effect on aedes albopictus, the effective protection rate can reach 95.83%, and the Badong buddleia Soxhlet alcohol extract can be used for preparing the mosquito repelling agent.

The utility model provides a kind of whitebark Aedes trapping and density monitoring device based on image recognition, it is related to insect trapping and identification technical field.A kind of whitebark Aedes trapping and density monitoring device based on image recognition, including mosquito trapping part, detection part, image acquisition part, control part and processing part, the mosquito trapping part includes upper mosquito suction fan, anti-escape funnel net and mosquito trapping assembly, the detection part includes fiber optic sensor, fiber optic sensor is provided with fiber optic sensor transmitting end and fiber optic sensor receiving end on it, the image acquisition part includes observation cabin, high-definition camera, observation board and lower mosquito suction fan, the observation cabin one side surface is provided with mosquito outlet.The above structure, realized the integration of mosquito trapping and species identification, without manual intervention can complete from trapping to identification process, improve work efficiency.

The invention discloses a method for identifying aedes albopictus and aedes aegypti based on a micro-well array and gene bar code technology, which is characterized in that a specific probe is designed aiming at a variation fragment of a 620bp target gene of a mitochondrial gene COI sequence of aedes albopictus and aedes aegypti, and the probe is a single-chain sequence rich in G basic groups; the method comprises the following steps of: preparing a probe, performing sulfydryl modification on the 5'end of the probe, preparing a hydrophilic micro-well array on a glass sheet by utilizing amination derivation, fixing by using a cross-linking agent to form a specific capture probe, hybridizing a sample and the capture probe, performing enzyme digestion, adding a silver nano-cluster aqueous solution, incubating, and developing, thereby realizing efficient, accurate and rapid detection on the aedes albopictus and aedes aegypti types. And the practical application value is very high.

The invention relates to a mosquito and egg trapping solution, and solves the problems of high monitoring cost and low monitoring efficiency of aedes albopictus in the prior art. The mosquito and egg trapping solution is prepared by fermenting 3%-10% by mass of bamboo leaf powder and 90%-97% by mass of water in a natural environment at the temperature of 20-24 DEG C for 24-96 hours. The preparation method comprises the following steps: step A, collecting bamboo leaves and drying; step B, grinding the dried bamboo leaves into powder; and step C, uniformly mixing 3%-10% by mass of bamboo leaf powder and 90%-97% by mass of water, and fermenting in a natural environment at the temperature of 20-24 DEG C for 24-96 hours. The Aedes albopictus monitoring device is simple in structure, easy in material taking and simple to manufacture, especially the Aedes albopictus can achieve the spawning effect of dozens of times of clear water within one day, the Aedes albopictus monitoring efficiency is greatly improved, meanwhile, dengue fever can be timely and accurately pre-warned and forecasted, the cost is low, and monitoring is convenient and efficient. In addition, the mosquito killer can be daily used for luring and killing mosquitoes.

The utility model discloses a long-term storage device for aedes albopictus eggs, and belongs to the technical field of mosquito prevention and control. A long-term storage device for aedes albopictus eggs comprises a constant-temperature and constant-humidity chamber and a transparent observation plate fixedly mounted on one side of the constant-temperature and constant-humidity chamber, a plurality of clamping grooves are fixedly mounted on the inner wall of the constant-temperature and constant-humidity chamber, mosquito egg filter paper is clamped in the clamping grooves, and the long-term storage device further comprises two mounting seats fixedly connected to the bottom of the constant-temperature and constant-humidity chamber. The mounting seat is rotationally connected with a reciprocating screw rod; according to the aedes albopictus ovum long-term preservation device, a gear motor is started to drive a reciprocating lead screw to rotate, an L-shaped sliding plate is driven to slide stably under the guiding effect of a guide rod, and meanwhile, the L-shaped sliding plate drives a vertical plate and a sponge strip in the vertical plate to slide and slide on a transparent observation plate for wiping; by means of the cleaning device, microorganisms and other impurities attached to the transparent observation plate can be wiped and removed, manual wiping is not needed, and automatic wiping can be completed without opening a sealing door.

The invention discloses an aedes albopictus drug resistance related gene mutation detection method based on a PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) technology, and belongs to a molecular detection technology, the aedes albopictus drug resistance related gene mutation detection method comprises the following steps: S1, extracting a genome DN A of single-head aedes albopictus, the genotype of a resistance related mutation site of the single-head aedes albopictus is known; s2, respectively obtaining electrophoretograms of resistance-related mutation site genotypes: firstly carrying out PCR amplification reaction, then carrying out enzyme digestion on a PCR amplification product, and carrying out electrophoresis detection on an enzyme digestion product; primers for PCR (Polymerase Chain Reaction) amplification of the VGSC 1016 are SEQ ID NO: 3 and SEQ ID NO: 4, and primers for amplification of the VGSC 1532 are SEQ ID NO: 7 and SEQ ID NO: 8; and S3, establishing a standard diagram. The method disclosed by the invention can be used for rapidly and effectively detecting the resistance of the aedes albopictus to the pyrethroid insecticides.

The application discloses a method, system and device for gender distinguishing of Aedes albopictus and a readable storage medium, and comprises the following steps: calibrating and selecting regions of each picture in the obtained data set; calibrating key features of each Aedes albopictus picture in the Aedes albopictus detection data set; based on the gender features of Aedes albopictus, pre-processing the key features of each Aedes albopictus region picture in the Aedes albopictus region data set to form an Aedes albopictus gender classification data set; obtaining an Aedes albopictus detection model, a key feature position detection model and an Aedes albopictus gender classification model; and detecting a to-be-detected picture by using the Aedes albopictus detection model, the key feature position detection model and the Aedes albopictus gender classification model in sequence to obtain a detection result. The application reduces and saves time and economic cost. The relative quantity of Aedes albopictus and the change in the quantity of the gender types of the Aedes albopictus caught can be used for monitoring the outbreak of the Aedes albopictus in the region and thus timely adjusting corresponding prevention and control measures.

The application discloses a construction method of Aedes albopictus Aa01 cell line and susceptible cell model of Decapod iridovirus 1, and belongs to the technical field of virology and aquaculture disease prevention and control, wherein the Aedes albopictus Aa01 cell line is preserved in the China Center for Type Culture Collection of Wuhan University, and the preservation number is CCTCC NO: C202648. The method for constructing the DIV1 susceptible cell model based on the above cell line comprises the following steps: culturing the Aedes albopictus Aa01 cell line, and subculturing or using for virus inoculation when the density reaches 80%-90%; inoculating the DIV1 into the Aedes albopictus Aa01 cell line, removing the virus liquid after virus adsorption, and adding cell maintenance liquid for continuous culture; and observing the cytopathic effect CPE, collecting the cell culture when the CPE reaches 70%-80%, and obtaining the amplified DIV1 virus liquid. The DIV1 susceptible cell model constructed by the method can solve the problem that there is currently a lack of stable and continuously subcultured DIV1 in vitro culture.

The invention provides an aedes albopictus trapping and density monitoring device based on image recognition, and relates to the technical field of insect trapping and recognition. An aedes albopictus trapping and density monitoring device based on image recognition comprises a mosquito trapping part, a detection part, an image acquisition part, a control part and a processing part, the mosquito trapping part comprises an upper mosquito suction fan, an anti-escape funnel net and a mosquito trapping assembly, and the detection part comprises an optical fiber sensor. An optical fiber sensor transmitting end and an optical fiber sensor receiving end are arranged on the optical fiber sensor, the image acquisition part comprises an observation cabin, a high-definition camera, an observation plate and a lower mosquito suction fan, and a mosquito outlet is formed in the surface of one side of the observation cabin. By means of the structure, integration of aedes albopictus trapping and species identification is achieved, the process from trapping to identification can be completed without manual intervention, working efficiency is improved, and the aedes albopictus trapping device plays an important role in the field of spreading dengue fever viruses and chikungunya fever viruses and emergency disposal of aedes albopictus at present.

The invention discloses application of Serpin protein based on mosquito salivary glands in preparation of antithrombotic drugs, and relates to the technical field of protein engineering, and the technical key points are as follows: the antithrombotic drugs comprise the Serpin protein extracted from the aedes albopictus salivary glands, and the Serpin protein can effectively prolong APTT, PT and TT of blood, reduce the content of Fib in the blood, and interact with a blood coagulation factor X. The invention also discloses a preparation method of the Serpin protein based on mosquito salivary glands, and the application of the Serpin protein based on mosquito salivary glands in preparation of antithrombotic drugs based on mosquito salivary glands in preparation of antithrombotic drugs based on mosquito salivary glands in preparation of antithrombotic drugs based on mosquito salivary glands. The Serpin protein of the aedes albopictus salivary gland can prolong PT, APTT and TT of blood in vitro and reduce the content of Fib in the blood; the Serpin protein has a therapeutic effect on formed thrombus in an in-vivo experiment, can relieve the blood coagulation degree and reduce the expression of blood coagulation factors, and can interact with a mouse blood coagulation factor X, so that the Serpin protein has important significance in understanding the anticoagulation mechanism of the Serpin protein and evaluating the potential of the Serpin protein as an antithrombotic therapeutic drug.

The invention belongs to the technical field of daily necessities, and provides a solid mosquito repellent product and a preparation method thereof. The solid mosquito repellent product comprises an essence base, a curing agent, an anti-cracking agent, a thickening agent, an emulsifying agent and a preservative, and the specific types and mass parts of all the components are limited. According to the invention, an essence base compounded by 2-undecanone and citronellal is adopted, and a sodium stearate-beeswax composite curing agent system and a carrageenan-xanthan gum double-thickening system are combined in a golden proportion, so that the volatilization rate is accurately regulated and controlled, and the mosquito repelling duration is as long as 15-60 days; through the synergistic effect of the emulsifier Tween 80 and the anti-cracking agent, nanoscale slow-release particles are formed, and uniform diffusion of mosquito-repelling components is ensured; an essence base compounded by citronellal, citronellol and peppermint oil can cover the slight grease flavor of 2-undecanone; the mosquito repelling effect is remarkable, the repelling rate of aedes albopictus reaches 98.2%, and the mosquito repellent is non-toxic, safe, pleasant in smell and free of skin irritation.

The present application relates to a kind of mosquito larvae development age identification method based on mosquito larvae head length and eye distance, belong to insect identification technical field.The present application provides a kind of mosquito larvae development age identification method, according to the numerical value of mosquito larvae head length and / or eye distance is determined.The present application takes mosquito larvae head length and eye distance as core morphological parameter, with the advantages of fast determination speed, wide application scene, improve the stability and accuracy of age determination.The present application further establishes the mathematical scoring model between head length and eye distance and development age, converts the morphological parameters measured into the score value that can be calculated, realizes the change from experience judgment to vector determination, with stronger objectivity, standardization degree and repeatability.In Toxorhynchites speciosus, Aedes albopictus and Culex quinquefasciatus, it has strong applicability.

The invention discloses an aedes albopictus control method, which uses cationic polyacrylamide to prepare a carrier-dsRNA compound for treatment, and can effectively kill aedes albopictus in various insect states. The dsRNA used in the method targets the aedes albopictus V-ATP enzyme H subunit gene, and is safe to other non-target organisms; meanwhile, the adopted cationic polyacrylamide has the advantages of high nucleic acid loading efficiency and good anti-degradation performance, also has the characteristics of low toxicity, high efficiency, safety and environmental protection, is good in biocompatibility, and ensures the safety of a drug applicator in the drug application process. The action mechanism of the method is different from that of conventional pesticides, drug resistance is not prone to being generated, raw material resources are rich, cost is low, the preparation method is simple, and the method is suitable for large-scale application and popularization.

The invention discloses an aedes albopictus drug resistance prediction and dynamic prevention and control decision system and method. The method comprises the following steps: S1, acquiring multi-source spatio-temporal data of a target monitoring area; s2, on the basis of real-time meteorological data and aedes albopictus population characteristics, constructing an aedes albopictus population generation replacement deduction model, and calculating a reproduction algebra; s3, on the basis of a population genetics principle, constructing a drug-resistant gene frequency evolution model introducing fitness cost, and calculating the frequency of a resistant allele in the current population; and S4, adopting a multi-objective optimization algorithm based on model predictive control, taking minimization of resistance growth and prevention and control cost and maximization of killing efficiency as objectives, performing deduction simulation, and selecting a strategy with the highest comprehensive score as a prevention and control method. According to the method, the resistance evolution law can be accurately quantified, the prevention and control requirements can be dynamically adapted, the problem of sharp increase of drug resistance caused by traditional empirical prevention and control is effectively solved, the service life of the insecticide is remarkably prolonged, and accurate and low-consumption long-term sustainable prevention and control are achieved.