Transgenic animals expressing salivary proteins

A technology of transgenic and transgenic pigs, which is applied in the field of animals that have been genetically modified to express the desired protein, and can solve the problem of not cloning the phytase gene

Inactive Publication Date: 2008-04-09
UNIVERSITY OF GUELPH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the authors did not clone the phytase gene to demonstrate that it is identical to the APPA gene

Method used

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  • Transgenic animals expressing salivary proteins
  • Transgenic animals expressing salivary proteins
  • Transgenic animals expressing salivary proteins

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Embodiment Construction

[0060] Description of the preferred embodiment

[0061] In the following description, a number of recombinant DNA technical terms are used. The following definitions are provided to enable a clearer understanding of this specification and the appended claims:

[0062] "Promoter" - a DNA sequence usually described as the 5' region of a gene and close to the start codon. Transcription of adjacent genes is initiated from the promoter region. If the promoter is an inducible promoter, the rate of transcription increases in response to the inducing agent. A constitutive promoter, on the other hand, initiates transcription of adjacent genes without additional regulation.

[0063] "Operably linked" - Nucleic acid sequences are "operably linked" when placed into a functional relationship with another nucleic acid sequence. For example, a promoter or enhancer is "operably linked" to a coding sequence if the promoter causes transcription of the sequence. Generally, "operably linked"...

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Abstract

The invention provides a transgenic animal having within its genome a transgene construct for gastrointestinal tract specific expression of a protein. In a preferred embodiment, the protein is a phytase or a homologue thereof. Such proteins may be heterologous and may be specifically expressed in the salivary gland of the animal by operably linking the nucleic acid sequence encoding the protein with regulatory sequence including a salivary gland protein promoter / enhancer. Also provided are methods of expressing and producing proteins using such nucleic acid constructs. Further, antibodies specific to such proteins and immunological diagnostic kits are also provided.

Description

field of invention [0001] The present invention relates to transgenic animals, and more particularly, to animals that have been genetically modified to express a desired protein. Background of the invention [0002] Phosphorus is an essential element for the growth of all organisms. In livestock production, where phosphorus deficiency has become the most prevalent mineral deficiency worldwide, inorganic phosphorus must be frequently added to the feed in order to obtain the desired growth performance of monogastric animals (eg pigs, poultry, etc.). [0003] Phytic acid or phytate (inositol 1,2,3,4,5,6-hexaphosphate dihydrogen salt) is the main storage form of phosphorus in cereals and legumes, accounting for 18%-88% of the total phosphorus content (Reddy et al., 1992). Phytase (phytase phosphate hydrolase) belongs to the class of phosphate monoester hydrolases: it catalyzes the hydrolysis of phytate (phytate) to the lower phosphate esters of inorganic monophosphate and inos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/06C12N15/00A01K67/027C12N9/16C12N15/85
CPCC12N15/8509A01K2267/01A01K2227/105A01K67/0275A01K2217/05C12N9/16C12N2830/008A01K2267/02A01K2227/108
Inventor C·W·福斯博格S·格罗万J·P·菲利浦
Owner UNIVERSITY OF GUELPH
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