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Attractant for promoting salivary protein secretion of mycophagous flour mites, and salivary protein collection method

A salivary protein and food habit technology, applied in the fields of attractants, botanical equipment and methods, material analysis by electromagnetic means, etc. Difficulty and other problems, to achieve the effect of reducing difficulty and increasing secretion

Active Publication Date: 2021-10-08
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Most of the mycophagous mites in the family Acaridae are small in size, the body length of adult mites is 0.1mm-0.9mm, and the mouthparts have degenerated. Insects with sucking mouthparts are collected by double-membrane clamping nutrient solution method; the body wall of acacia mites is thin, the jaw body is less than 0.01 mm, and the degree of ossification is low. It is very easy to break during the process of dissecting the jaw body, and it is difficult to collect enough. A large amount of oral secretions increase the difficulty of obtaining high-concentration and high-quality salivary proteins

Method used

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  • Attractant for promoting salivary protein secretion of mycophagous flour mites, and salivary protein collection method
  • Attractant for promoting salivary protein secretion of mycophagous flour mites, and salivary protein collection method
  • Attractant for promoting salivary protein secretion of mycophagous flour mites, and salivary protein collection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] S1: placing the base 1 of the petri dish in the second petri dish 5;

[0051] S2: add clear water to the height of 1 / 2 of the base 1 of the petri dish in the second petri dish 5;

[0052] S3: Place the solvent in base 1 of the petri dish, the mass percentage of Tween-20 in the solvent is 0.1%, the mass percentage of sucrose is 5%, and the rest is water; the pH of the solvent is 6-7.5;

[0053] S4: covering the parafilm 2 on the base 1 of the petri dish with the solvent;

[0054] S5: Use an 80-mesh sieve to collect 0.1 g each of adult tyrophagous mite and Rhizopus robinii, and put them in a 2.0-mL centrifuge tube to starve for 24 hours. 300-mesh gauze 3 is collected, the moisture on the gauze 3 is blotted, and it is buckled on the sealing film 2, so that fungal-eating acaroid mites are located on the sealing film 2;

[0055] S6: Cover the upper cover 4 of the petri dish on the gauze 3 to obtain the assembled saliva collection device;

[0056] S7: Place the assembled s...

Embodiment 2

[0059] The mass percentage of sucrose in step S3 of this embodiment is 10%, and the rest are the same as in embodiment 1.

Embodiment 3

[0061] The mass percentage of sucrose in step S3 of this embodiment is 20%, and the rest are the same as in embodiment 1.

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Abstract

The invention provides an attractant for promoting salivary protein secretion of mycophagous flour mites, and a salivary protein collection method. The attractant comprises a solvent and an attractant component dissolved in the solvent; the solvent comprises a sucrose aqueous solution; and the attractant component is prepared from 3-octanone and alpha-terpinene. According to the attractant for promoting salivary protein secretion of the mycophagous flour mites, provided by the invention, through the synergistic effect of the sucrose aqueous solution, the 3-octanone and the alpha-terpinene, the mycophagous flour mites are attracted by utilizing the volatility of the 3-octanone and the alpha-terpinene; and 3-octanone, alpha-terpinene and the sucrose aqueous solution are used for stimulating the lured mycophagous flour mites to secrete salivary proteins, so that the secretion amount of the salivary proteins is increased, and the difficulty of obtaining the flour mite salivary protein is reduced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an attractant for promoting the secretion of salivary protein of fungal-eating mites and a method for collecting salivary protein. Background technique [0002] Acarid belongs to Arachnida (Arachinida), Acari subclass (Acari), currently has 5500 genera and 1200 subgenus, 124 general families and 540 families. my country is a big producer of edible fungi and also a big exporter of edible fungi. Mites are an important group that harms edible fungi. There are many types of mites. According to statistics, there are currently 21 families and 44 species of harmful mites found in my country that are related to the cultivation of edible fungi. Among them, there are as many as 13 species of Acarididae, including important harmful mites: Tyrophagus putrescentiae, T.longior, Rhizoglyphus robini, Acarus siro )wait. [0003] Mites can eat mycelium and damage fruiting bodies. The symptoms of host ...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01N35/02A01N27/00A01P19/00G01N1/10G01N27/62
CPCA01N43/16A01N35/02A01N27/00G01N1/10G01N27/62
Inventor 曲绍轩李辉平马林骆昕侯立娟蒋宁林金盛姜建新
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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