Agglutinin chip for identifying breast cancer based on sialoprotein, reagent kit and application of reagent kit

Abstract

The relates to an agglutinin chip used for identifying cancer, in particular to an agglutinin chip for identifying breast cancer based on sialoprotein, a reagent kit and application of the reagent kit, and aims at providing an agglutinin chip for identifying breast cancer in a non-destructive mode through the change of carbohydrate chains in saliva, a reagent kit and application of the reagent kit. The agglutinin chip for identifying breast cancer based on sialoprotein comprises an agglutinin testing probe set. The agglutinin testing probe set at least comprises a combination of PHA-E+L, LTL, BS-I, MAL-I, LCA, BPL, PTL-II, NPA and GSL-I, or at least comprises a combination of LTL, BS-I, MAL-I, LCA, BPL, NPA and GSL-I. By means of the agglutinin chip, differentially-expressed glycoprotein carbohydrate chain structures in a patient saliva sample can be rapidly detected, whether a corresponding person suffers from a breast tumor or not is detected, and whether the breast tumor is breast cancer or not is determined; besides, saliva is adopted as an object to be detected and is easy and convenient to collect, and health risks existing when a serum sample is collected for detection in the prior art are avoided.

Description

technical field [0001] The invention relates to a lectin chip for discriminating cancer, in particular to a lectin chip for discriminating breast cancer based on protein and a kit thereof. Background technique [0002] Breast cancer is the most common type of malignant tumor in the world, which seriously endangers the physical and mental health of women. In recent years, the incidence of breast cancer in my country has shown a clear upward trend. IARC predicts that the number of female breast cancer patients in my country will reach 234,000 in 2030, and the mortality rate will increase by 47.94% compared with 2008. Due to the rising incidence rate and young age of onset Advances in breast cancer screening, diagnosis, and treatment have been receiving much attention due to the globalization and diversification of the disease. In areas with high breast cancer incidence in Europe and the United States, the reduction in mortality has benefited to a certain extent from the census...

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Problems solved by technology

However, the above studies all used tissue or serum as samples, and little is known about the changes in protein glycosylation levels in saliva, and it has not been applied to the early screening of breast cancer women

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