Agglutinin chip for identifying breast cancer based on sialoprotein, reagent kit and application of reagent kit

A salivary protein and lectin technology, which is applied in the field of protein-based lectin chips and kits for breast cancer identification, can solve problems such as early screening of breast cancer female patients that have not yet been applied, and achieve easy collection and avoid health problems. risk effect

Active Publication Date: 2016-09-07
深圳格道糖生物技术有限公司
View PDF3 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above studies all used tissue or serum as samples, and little is known about the changes in protein glycosylation levels in saliva, and it has not been applied to the early screening of breast cancer women

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Agglutinin chip for identifying breast cancer based on sialoprotein, reagent kit and application of reagent kit
  • Agglutinin chip for identifying breast cancer based on sialoprotein, reagent kit and application of reagent kit
  • Agglutinin chip for identifying breast cancer based on sialoprotein, reagent kit and application of reagent kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] The present invention will be further described by the specific examples given below, but not as a limitation of the present invention.

[0025] The present invention screens lectin probes for screening female patients with breast cancer from 37 lectins (as shown in Table 1).

[0026] Table 1

[0027]

[0028]

[0029] The reagent materials used in the present invention include: protease inhibitors, DMSO, Cy3, Cy5 fluorescent dyes, SephadexgG-25 column, bovine serum albumin (BSA), glass substrate, oxysilane reagent (GPTS), Bradford reagent, PVDF Membrane, Tween-20, and common reagents of domestic analytical grade.

[0030] The equipment used includes: ultra-fast refrigerated centrifuge 5804R: Eppendorf, Germany; micronucleic acid and protein analyzer: Implen, Germany; chip spotting instrument: Boao Jingxin SmartArrayer48 spotting instrument; biochip scanner 4000B: Axon, USA; chip Hybridization Box HL-2000: American UVP Company. Immobilon-P semi-dry film transfe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The relates to an agglutinin chip used for identifying cancer, in particular to an agglutinin chip for identifying breast cancer based on sialoprotein, a reagent kit and application of the reagent kit, and aims at providing an agglutinin chip for identifying breast cancer in a non-destructive mode through the change of carbohydrate chains in saliva, a reagent kit and application of the reagent kit. The agglutinin chip for identifying breast cancer based on sialoprotein comprises an agglutinin testing probe set. The agglutinin testing probe set at least comprises a combination of PHA-E+L, LTL, BS-I, MAL-I, LCA, BPL, PTL-II, NPA and GSL-I, or at least comprises a combination of LTL, BS-I, MAL-I, LCA, BPL, NPA and GSL-I. By means of the agglutinin chip, differentially-expressed glycoprotein carbohydrate chain structures in a patient saliva sample can be rapidly detected, whether a corresponding person suffers from a breast tumor or not is detected, and whether the breast tumor is breast cancer or not is determined; besides, saliva is adopted as an object to be detected and is easy and convenient to collect, and health risks existing when a serum sample is collected for detection in the prior art are avoided.

Description

technical field [0001] The invention relates to a lectin chip for discriminating cancer, in particular to a lectin chip for discriminating breast cancer based on protein and a kit thereof. Background technique [0002] Breast cancer is the most common type of malignant tumor in the world, which seriously endangers the physical and mental health of women. In recent years, the incidence of breast cancer in my country has shown a clear upward trend. IARC predicts that the number of female breast cancer patients in my country will reach 234,000 in 2030, and the mortality rate will increase by 47.94% compared with 2008. Due to the rising incidence rate and young age of onset Advances in breast cancer screening, diagnosis, and treatment have been receiving much attention due to the globalization and diversification of the disease. In areas with high breast cancer incidence in Europe and the United States, the reduction in mortality has benefited to a certain extent from the census...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/68
CPCG01N33/57415G01N33/68
Inventor 李铮刘夏薇于汉杰钟耀刚舒健阳佳君
Owner 深圳格道糖生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap