Cloned weakening strain of chicken virus mycoplasma

A kind of Mycoplasma gallisepticum, weakening technology, applied in the direction of bacteria, etc., can solve the problem of weak virulence, poor immunogenicity and so on

Inactive Publication Date: 2003-11-19
江苏省农业科学院畜牧兽医研究所
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  • Summary
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AI Technical Summary

Problems solved by technology

S6 strain is a poisoned strain of MG. Domestic immunopathological tests have also proved that its virulence is not strong and its immunogenicity is poor.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] The cultivation and identification of the present invention are further described below:

[0023] 1. Common media formulations for cell-free culture

[0024] a.

[0025] Beef Heart Soup 1000ml

[0026] Niacinamide 30mg

[0027] Glucose 10g

[0028] Bannon Horse Serum 200ml

[0029] Phenol red 25mg

[0030] 25% Yeast Extract 40ml

[0031] pH 8.0

[0032] * Solid Medium 1% Agar

[0033] B

[0034] Beef Heart Digestive Soup 1000ml

[0035] Milk protein hydrolyzate 5g

[0036] NaCl 5g

[0037] 25% yeast extract 50-100ml

[0038] NAD(COI) 0.02g

[0039] Phenol red 0.01-0.02g

[0040] Arginine 0.1-0.2g

[0041] Eagles minimum needs vitamins (100×) 10ml in the culture medium

[0042] Pig Serum 150ml

[0043]Thallium acetate 0.25g

[0044] Penicillin 1000u / ml

[0045] PH7.2

[004...

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Abstract

The strain is a hypotoxic strain with immunogen formed by S6 strain and through thrice solid cloning, then acellular culture and weakening to over 168 generation. The strain is safe, without restoring its strong toxicity, easy to test, and has relatively strong immunogen for preventing chicken's chronic respiratory track disease. The strain is preserved in Common Microbe Center of Chinese Microbial Vaccine Storing and Management Commission with the stroing number being CGMCC No.0397.

Description

Technical field: [0001] The present invention belongs to novel microbial strains. Background technique: [0002] Mycoplasma gallisepticum (Mycoplasma gallisepticum, MG) is the main pathogen of chicken chronic respiratory disease (Chronic Respiratory Disease of Chicken, CRD), because of its extensive pathogenicity and serious economic loss, it has attracted attention. In recent years, the research on MG has developed from routine pathogen isolation, serological tests, immunization and drug control to molecular biology research, which has enabled people to understand the properties of MG more deeply in essence and provided new information for the control of MG infection. technology. There are as many as 20 kinds of mycoplasma isolated from poultry, but there are three kinds of mycoplasma that harm chickens: MG, mycoplasma synoviae (M.Synoviae.MS), and mycoplasma turkey (M.Meleagritis MM). The pathogenicity of different MS isolates is quite different, and the main lesions are...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20
Inventor 邵国青钱建飞侯继波何家惠还红华王继春倪艳秀
Owner 江苏省农业科学院畜牧兽医研究所
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